Mycobacterium phage Hegedechwinu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LNT6|A0A6B9LNT6_9CAUD Uncharacterized protein OS=Mycobacterium phage Hegedechwinu OX=2686469 GN=51 PE=4 SV=1
MM1 pKa = 7.22MSDD4 pKa = 3.93PVRR7 pKa = 11.84GAIQASLDD15 pKa = 3.5AMGDD19 pKa = 3.44GWQVAHH25 pKa = 5.73YY26 pKa = 9.08VVVVGLEE33 pKa = 4.17RR34 pKa = 11.84IDD36 pKa = 4.1GDD38 pKa = 4.67RR39 pKa = 11.84MDD41 pKa = 5.5LGATTVITPIGQAGYY56 pKa = 8.35VTDD59 pKa = 4.2GLVNRR64 pKa = 11.84YY65 pKa = 7.35WDD67 pKa = 3.73EE68 pKa = 4.28SSGEE72 pKa = 3.97

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LVC3|A0A6B9LVC3_9CAUD Uncharacterized protein OS=Mycobacterium phage Hegedechwinu OX=2686469 GN=96 PE=4 SV=1
MM1 pKa = 7.8AGTAVLTPDD10 pKa = 5.37GIDD13 pKa = 3.45TLVTAAEE20 pKa = 4.08AASLCGVTTSTIYY33 pKa = 10.19VWVNRR38 pKa = 11.84GALAPSGKK46 pKa = 9.47NRR48 pKa = 11.84LGHH51 pKa = 5.16NVYY54 pKa = 10.31RR55 pKa = 11.84VLDD58 pKa = 3.67VAKK61 pKa = 10.59AEE63 pKa = 4.07HH64 pKa = 6.08ATRR67 pKa = 11.84AKK69 pKa = 10.42ARR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 4.63RR74 pKa = 3.93

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

112

0

112

18647

30

1175

166.5

18.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.785 ± 0.421

1.115 ± 0.14

6.607 ± 0.232

5.76 ± 0.271

2.891 ± 0.212

8.908 ± 0.572

2.215 ± 0.166

4.424 ± 0.177

3.443 ± 0.2

7.476 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.258 ± 0.118

3.314 ± 0.138

6.06 ± 0.208

3.582 ± 0.207

6.677 ± 0.373

5.958 ± 0.247

6.36 ± 0.259

7.218 ± 0.252

2.354 ± 0.149

2.596 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski