Amino acid dipepetide frequency for Anaerocolumna cellulosilytica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.454AlaAla: 5.454 ± 0.082	0.832AlaCys: 0.832 ± 0.026	3.632AlaAsp: 3.632 ± 0.048	4.369AlaGlu: 4.369 ± 0.053	2.923AlaPhe: 2.923 ± 0.05	5.072AlaGly: 5.072 ± 0.069	0.909AlaHis: 0.909 ± 0.026	5.128AlaIle: 5.128 ± 0.059	4.543AlaLys: 4.543 ± 0.057	6.272AlaLeu: 6.272 ± 0.073	1.797AlaMet: 1.797 ± 0.038	2.615AlaAsn: 2.615 ± 0.042	1.679AlaPro: 1.679 ± 0.041	1.627AlaGln: 1.627 ± 0.032	2.282AlaArg: 2.282 ± 0.041	3.624AlaSer: 3.624 ± 0.053	2.964AlaThr: 2.964 ± 0.056	5.096AlaVal: 5.096 ± 0.063	0.536AlaTrp: 0.536 ± 0.02	2.72AlaTyr: 2.72 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.695CysAla: 0.695 ± 0.022	0.206CysCys: 0.206 ± 0.011	0.697CysAsp: 0.697 ± 0.021	0.744CysGlu: 0.744 ± 0.024	0.583CysPhe: 0.583 ± 0.021	1.056CysGly: 1.056 ± 0.032	0.29CysHis: 0.29 ± 0.013	1.122CysIle: 1.122 ± 0.028	0.871CysLys: 0.871 ± 0.026	0.987CysLeu: 0.987 ± 0.025	0.355CysMet: 0.355 ± 0.015	0.653CysAsn: 0.653 ± 0.021	0.48CysPro: 0.48 ± 0.026	0.33CysGln: 0.33 ± 0.016	0.458CysArg: 0.458 ± 0.017	0.863CysSer: 0.863 ± 0.023	0.67CysThr: 0.67 ± 0.023	0.694CysVal: 0.694 ± 0.021	0.092CysTrp: 0.092 ± 0.008	0.524CysTyr: 0.524 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	2.992AspAla: 2.992 ± 0.051	0.692AspCys: 0.692 ± 0.022	2.267AspAsp: 2.267 ± 0.042	3.797AspGlu: 3.797 ± 0.062	2.775AspPhe: 2.775 ± 0.045	3.657AspGly: 3.657 ± 0.061	0.574AspHis: 0.574 ± 0.021	5.257AspIle: 5.257 ± 0.065	4.353AspLys: 4.353 ± 0.054	4.471AspLeu: 4.471 ± 0.053	1.64AspMet: 1.64 ± 0.032	2.904AspAsn: 2.904 ± 0.047	1.301AspPro: 1.301 ± 0.031	0.958AspGln: 0.958 ± 0.027	1.896AspArg: 1.896 ± 0.038	3.353AspSer: 3.353 ± 0.044	3.404AspThr: 3.404 ± 0.05	3.128AspVal: 3.128 ± 0.049	0.591AspTrp: 0.591 ± 0.019	3.008AspTyr: 3.008 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	5.187GluAla: 5.187 ± 0.068	0.695GluCys: 0.695 ± 0.026	4.069GluAsp: 4.069 ± 0.054	7.033GluGlu: 7.033 ± 0.096	2.73GluPhe: 2.73 ± 0.043	4.312GluGly: 4.312 ± 0.064	1.296GluHis: 1.296 ± 0.033	6.037GluIle: 6.037 ± 0.075	6.33GluLys: 6.33 ± 0.082	7.132GluLeu: 7.132 ± 0.083	1.912GluMet: 1.912 ± 0.035	4.16GluAsn: 4.16 ± 0.057	1.718GluPro: 1.718 ± 0.037	2.802GluGln: 2.802 ± 0.053	2.847GluArg: 2.847 ± 0.053	3.594GluSer: 3.594 ± 0.055	3.582GluThr: 3.582 ± 0.051	4.62GluVal: 4.62 ± 0.058	0.629GluTrp: 0.629 ± 0.024	3.609GluTyr: 3.609 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	2.409PheAla: 2.409 ± 0.044	0.615PheCys: 0.615 ± 0.021	2.53PheAsp: 2.53 ± 0.041	2.696PheGlu: 2.696 ± 0.049	1.936PhePhe: 1.936 ± 0.043	2.819PheGly: 2.819 ± 0.048	0.896PheHis: 0.896 ± 0.026	3.675PheIle: 3.675 ± 0.061	2.659PheLys: 2.659 ± 0.04	4.182PheLeu: 4.182 ± 0.061	1.176PheMet: 1.176 ± 0.028	2.113PheAsn: 2.113 ± 0.033	1.276PhePro: 1.276 ± 0.029	1.426PheGln: 1.426 ± 0.029	1.554PheArg: 1.554 ± 0.026	3.063PheSer: 3.063 ± 0.051	2.611PheThr: 2.611 ± 0.046	2.711PheVal: 2.711 ± 0.046	0.414PheTrp: 0.414 ± 0.018	2.081PheTyr: 2.081 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	4.12GlyAla: 4.12 ± 0.064	0.957GlyCys: 0.957 ± 0.03	3.199GlyAsp: 3.199 ± 0.046	4.442GlyGlu: 4.442 ± 0.062	3.15GlyPhe: 3.15 ± 0.054	4.488GlyGly: 4.488 ± 0.071	1.159GlyHis: 1.159 ± 0.029	6.543GlyIle: 6.543 ± 0.07	5.062GlyLys: 5.062 ± 0.063	5.664GlyLeu: 5.664 ± 0.073	2.042GlyMet: 2.042 ± 0.04	3.391GlyAsn: 3.391 ± 0.055	1.138GlyPro: 1.138 ± 0.029	1.803GlyGln: 1.803 ± 0.038	2.558GlyArg: 2.558 ± 0.05	3.961GlySer: 3.961 ± 0.057	4.006GlyThr: 4.006 ± 0.062	4.462GlyVal: 4.462 ± 0.07	0.614GlyTrp: 0.614 ± 0.025	3.318GlyTyr: 3.318 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	0.891HisAla: 0.891 ± 0.026	0.266HisCys: 0.266 ± 0.012	0.783HisAsp: 0.783 ± 0.026	1.012HisGlu: 1.012 ± 0.026	0.841HisPhe: 0.841 ± 0.022	1.172HisGly: 1.172 ± 0.03	0.375HisHis: 0.375 ± 0.016	1.502HisIle: 1.502 ± 0.031	1.205HisLys: 1.205 ± 0.03	1.527HisLeu: 1.527 ± 0.031	0.484HisMet: 0.484 ± 0.019	0.895HisAsn: 0.895 ± 0.026	0.726HisPro: 0.726 ± 0.023	0.535HisGln: 0.535 ± 0.02	0.668HisArg: 0.668 ± 0.022	1.051HisSer: 1.051 ± 0.029	0.982HisThr: 0.982 ± 0.028	0.884HisVal: 0.884 ± 0.024	0.19HisTrp: 0.19 ± 0.011	0.822HisTyr: 0.822 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.581IleAla: 5.581 ± 0.068	1.144IleCys: 1.144 ± 0.03	4.438IleAsp: 4.438 ± 0.052	5.565IleGlu: 5.565 ± 0.061	3.286IlePhe: 3.286 ± 0.05	5.238IleGly: 5.238 ± 0.069	1.594IleHis: 1.594 ± 0.04	6.711IleIle: 6.711 ± 0.091	6.121IleLys: 6.121 ± 0.062	8.063IleLeu: 8.063 ± 0.075	2.019IleMet: 2.019 ± 0.037	4.326IleAsn: 4.326 ± 0.056	3.266IlePro: 3.266 ± 0.044	2.716IleGln: 2.716 ± 0.044	3.378IleArg: 3.378 ± 0.045	5.796IleSer: 5.796 ± 0.075	5.357IleThr: 5.357 ± 0.062	5.096IleVal: 5.096 ± 0.064	0.635IleTrp: 0.635 ± 0.021	3.382IleTyr: 3.382 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.055LysAla: 5.055 ± 0.065	0.651LysCys: 0.651 ± 0.021	4.434LysAsp: 4.434 ± 0.052	7.91LysGlu: 7.91 ± 0.088	2.141LysPhe: 2.141 ± 0.038	4.481LysGly: 4.481 ± 0.058	1.138LysHis: 1.138 ± 0.026	5.607LysIle: 5.607 ± 0.073	6.499LysLys: 6.499 ± 0.077	6.523LysLeu: 6.523 ± 0.065	1.952LysMet: 1.952 ± 0.036	4.455LysAsn: 4.455 ± 0.053	2.049LysPro: 2.049 ± 0.037	2.775LysGln: 2.775 ± 0.056	3.022LysArg: 3.022 ± 0.049	4.139LysSer: 4.139 ± 0.064	4.101LysThr: 4.101 ± 0.052	4.812LysVal: 4.812 ± 0.061	0.587LysTrp: 0.587 ± 0.021	3.265LysTyr: 3.265 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	6.066LeuAla: 6.066 ± 0.068	1.298LeuCys: 1.298 ± 0.032	4.777LeuAsp: 4.777 ± 0.057	6.516LeuGlu: 6.516 ± 0.08	4.012LeuPhe: 4.012 ± 0.055	5.704LeuGly: 5.704 ± 0.068	1.624LeuHis: 1.624 ± 0.038	6.94LeuIle: 6.94 ± 0.08	7.414LeuLys: 7.414 ± 0.071	9.683LeuLeu: 9.683 ± 0.124	2.416LeuMet: 2.416 ± 0.047	4.874LeuAsn: 4.874 ± 0.066	3.499LeuPro: 3.499 ± 0.056	3.007LeuGln: 3.007 ± 0.051	3.441LeuArg: 3.441 ± 0.052	7.034LeuSer: 7.034 ± 0.07	5.612LeuThr: 5.612 ± 0.069	5.373LeuVal: 5.373 ± 0.07	0.781LeuTrp: 0.781 ± 0.024	3.989LeuTyr: 3.989 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	1.86MetAla: 1.86 ± 0.038	0.251MetCys: 0.251 ± 0.014	1.69MetAsp: 1.69 ± 0.033	2.267MetGlu: 2.267 ± 0.041	0.985MetPhe: 0.985 ± 0.025	1.824MetGly: 1.824 ± 0.035	0.411MetHis: 0.411 ± 0.017	1.991MetIle: 1.991 ± 0.034	2.372MetLys: 2.372 ± 0.041	2.466MetLeu: 2.466 ± 0.046	0.66MetMet: 0.66 ± 0.019	1.683MetAsn: 1.683 ± 0.031	0.954MetPro: 0.954 ± 0.021	0.861MetGln: 0.861 ± 0.022	0.924MetArg: 0.924 ± 0.025	1.588MetSer: 1.588 ± 0.031	1.356MetThr: 1.356 ± 0.03	1.7MetVal: 1.7 ± 0.034	0.208MetTrp: 0.208 ± 0.012	0.95MetTyr: 0.95 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.952AsnAla: 2.952 ± 0.047	0.676AsnCys: 0.676 ± 0.022	2.346AsnAsp: 2.346 ± 0.038	3.401AsnGlu: 3.401 ± 0.049	2.064AsnPhe: 2.064 ± 0.042	3.691AsnGly: 3.691 ± 0.062	1.047AsnHis: 1.047 ± 0.023	4.59AsnIle: 4.59 ± 0.063	3.821AsnLys: 3.821 ± 0.054	4.771AsnLeu: 4.771 ± 0.053	1.389AsnMet: 1.389 ± 0.031	2.964AsnAsn: 2.964 ± 0.052	2.145AsnPro: 2.145 ± 0.037	2.034AsnGln: 2.034 ± 0.042	2.252AsnArg: 2.252 ± 0.048	3.209AsnSer: 3.209 ± 0.054	3.226AsnThr: 3.226 ± 0.048	2.942AsnVal: 2.942 ± 0.048	0.508AsnTrp: 0.508 ± 0.02	2.51AsnTyr: 2.51 ± 0.045	0.0AsnXaa: 0.0 ± 0.0
Pro	1.993ProAla: 1.993 ± 0.04	0.356ProCys: 0.356 ± 0.017	1.98ProAsp: 1.98 ± 0.039	2.638ProGlu: 2.638 ± 0.047	1.549ProPhe: 1.549 ± 0.031	1.97ProGly: 1.97 ± 0.041	0.545ProHis: 0.545 ± 0.02	2.404ProIle: 2.404 ± 0.04	1.931ProLys: 1.931 ± 0.039	2.686ProLeu: 2.686 ± 0.042	0.801ProMet: 0.801 ± 0.023	1.372ProAsn: 1.372 ± 0.031	0.689ProPro: 0.689 ± 0.021	0.92ProGln: 0.92 ± 0.024	0.862ProArg: 0.862 ± 0.022	1.881ProSer: 1.881 ± 0.034	1.715ProThr: 1.715 ± 0.047	2.632ProVal: 2.632 ± 0.036	0.292ProTrp: 0.292 ± 0.013	1.474ProTyr: 1.474 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.021GlnAla: 2.021 ± 0.036	0.325GlnCys: 0.325 ± 0.015	1.505GlnAsp: 1.505 ± 0.029	2.606GlnGlu: 2.606 ± 0.053	1.233GlnPhe: 1.233 ± 0.028	1.905GlnGly: 1.905 ± 0.043	0.427GlnHis: 0.427 ± 0.016	2.536GlnIle: 2.536 ± 0.046	2.632GlnLys: 2.632 ± 0.038	3.009GlnLeu: 3.009 ± 0.048	0.924GlnMet: 0.924 ± 0.026	1.723GlnAsn: 1.723 ± 0.034	0.84GlnPro: 0.84 ± 0.025	1.061GlnGln: 1.061 ± 0.029	1.196GlnArg: 1.196 ± 0.034	1.79GlnSer: 1.79 ± 0.034	1.716GlnThr: 1.716 ± 0.035	2.122GlnVal: 2.122 ± 0.044	0.309GlnTrp: 0.309 ± 0.013	1.463GlnTyr: 1.463 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	2.042ArgAla: 2.042 ± 0.041	0.452ArgCys: 0.452 ± 0.018	1.831ArgAsp: 1.831 ± 0.038	3.071ArgGlu: 3.071 ± 0.056	1.739ArgPhe: 1.739 ± 0.038	2.044ArgGly: 2.044 ± 0.043	0.609ArgHis: 0.609 ± 0.024	3.508ArgIle: 3.508 ± 0.058	2.993ArgLys: 2.993 ± 0.048	3.753ArgLeu: 3.753 ± 0.055	1.159ArgMet: 1.159 ± 0.024	2.172ArgAsn: 2.172 ± 0.039	1.012ArgPro: 1.012 ± 0.028	1.341ArgGln: 1.341 ± 0.032	1.619ArgArg: 1.619 ± 0.041	1.846ArgSer: 1.846 ± 0.032	1.985ArgThr: 1.985 ± 0.034	2.231ArgVal: 2.231 ± 0.039	0.337ArgTrp: 0.337 ± 0.015	1.835ArgTyr: 1.835 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.73SerAla: 3.73 ± 0.053	0.737SerCys: 0.737 ± 0.022	3.259SerAsp: 3.259 ± 0.046	4.114SerGlu: 4.114 ± 0.053	3.027SerPhe: 3.027 ± 0.043	4.731SerGly: 4.731 ± 0.069	1.048SerHis: 1.048 ± 0.027	5.366SerIle: 5.366 ± 0.06	4.448SerLys: 4.448 ± 0.055	5.95SerLeu: 5.95 ± 0.067	1.748SerMet: 1.748 ± 0.037	3.229SerAsn: 3.229 ± 0.047	1.809SerPro: 1.809 ± 0.036	1.852SerGln: 1.852 ± 0.038	2.259SerArg: 2.259 ± 0.041	4.038SerSer: 4.038 ± 0.066	3.171SerThr: 3.171 ± 0.048	4.16SerVal: 4.16 ± 0.058	0.549SerTrp: 0.549 ± 0.019	2.928SerTyr: 2.928 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	4.138ThrAla: 4.138 ± 0.061	0.588ThrCys: 0.588 ± 0.02	3.081ThrAsp: 3.081 ± 0.051	3.991ThrGlu: 3.991 ± 0.051	2.366ThrPhe: 2.366 ± 0.042	4.58ThrGly: 4.58 ± 0.063	0.845ThrHis: 0.845 ± 0.025	4.791ThrIle: 4.791 ± 0.058	3.722ThrLys: 3.722 ± 0.058	5.403ThrLeu: 5.403 ± 0.07	1.418ThrMet: 1.418 ± 0.03	2.601ThrAsn: 2.601 ± 0.044	2.17ThrPro: 2.17 ± 0.042	1.443ThrGln: 1.443 ± 0.031	1.797ThrArg: 1.797 ± 0.036	3.383ThrSer: 3.383 ± 0.058	3.131ThrThr: 3.131 ± 0.055	4.353ThrVal: 4.353 ± 0.063	0.464ThrTrp: 0.464 ± 0.023	2.59ThrTyr: 2.59 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	3.882ValAla: 3.882 ± 0.067	0.891ValCys: 0.891 ± 0.026	3.299ValAsp: 3.299 ± 0.04	4.133ValGlu: 4.133 ± 0.052	2.95ValPhe: 2.95 ± 0.047	3.699ValGly: 3.699 ± 0.056	1.0ValHis: 1.0 ± 0.025	5.632ValIle: 5.632 ± 0.073	4.836ValLys: 4.836 ± 0.057	6.401ValLeu: 6.401 ± 0.073	1.728ValMet: 1.728 ± 0.032	3.297ValAsn: 3.297 ± 0.046	2.141ValPro: 2.141 ± 0.035	1.76ValGln: 1.76 ± 0.031	2.342ValArg: 2.342 ± 0.037	4.531ValSer: 4.531 ± 0.06	4.226ValThr: 4.226 ± 0.068	4.24ValVal: 4.24 ± 0.067	0.544ValTrp: 0.544 ± 0.017	2.735ValTyr: 2.735 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.468TrpAla: 0.468 ± 0.022	0.126TrpCys: 0.126 ± 0.01	0.533TrpAsp: 0.533 ± 0.021	0.698TrpGlu: 0.698 ± 0.021	0.448TrpPhe: 0.448 ± 0.019	0.658TrpGly: 0.658 ± 0.023	0.159TrpHis: 0.159 ± 0.011	0.651TrpIle: 0.651 ± 0.022	0.65TrpLys: 0.65 ± 0.023	0.833TrpLeu: 0.833 ± 0.025	0.238TrpMet: 0.238 ± 0.012	0.57TrpAsn: 0.57 ± 0.02	0.202TrpPro: 0.202 ± 0.011	0.318TrpGln: 0.318 ± 0.015	0.361TrpArg: 0.361 ± 0.016	0.523TrpSer: 0.523 ± 0.024	0.39TrpThr: 0.39 ± 0.018	0.469TrpVal: 0.469 ± 0.016	0.119TrpTrp: 0.119 ± 0.009	0.386TrpTyr: 0.386 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.536TyrAla: 2.536 ± 0.048	0.615TyrCys: 0.615 ± 0.017	2.666TyrAsp: 2.666 ± 0.057	3.29TyrGlu: 3.29 ± 0.054	2.104TyrPhe: 2.104 ± 0.04	2.977TyrGly: 2.977 ± 0.047	0.865TyrHis: 0.865 ± 0.025	3.72TyrIle: 3.72 ± 0.051	3.135TyrLys: 3.135 ± 0.043	4.282TyrLeu: 4.282 ± 0.053	1.142TyrMet: 1.142 ± 0.024	2.543TyrAsn: 2.543 ± 0.047	1.597TyrPro: 1.597 ± 0.034	1.773TyrGln: 1.773 ± 0.036	1.864TyrArg: 1.864 ± 0.036	2.884TyrSer: 2.884 ± 0.049	2.648TyrThr: 2.648 ± 0.05	2.542TyrVal: 2.542 ± 0.041	0.405TyrTrp: 0.405 ± 0.018	2.322TyrTyr: 2.322 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4494 proteins (1532155 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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