Wigglesworthia glossinidia brevipalpis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Wigglesworthia; Wigglesworthia glossinidia

Average proteome isoelectric point is 8.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8D3B7|RL20_WIGBR 50S ribosomal protein L20 OS=Wigglesworthia glossinidia brevipalpis OX=36870 GN=rplT PE=3 SV=1
MM1 pKa = 6.95STIEE5 pKa = 4.32EE6 pKa = 4.42SVKK9 pKa = 10.91SIIAEE14 pKa = 3.7QLGVKK19 pKa = 10.09KK20 pKa = 10.98EE21 pKa = 4.08EE22 pKa = 4.1VTNNASFVDD31 pKa = 4.06DD32 pKa = 5.33LGADD36 pKa = 3.42SLDD39 pKa = 3.64TVEE42 pKa = 5.9LVMALEE48 pKa = 4.51EE49 pKa = 4.26EE50 pKa = 4.66FDD52 pKa = 3.79TEE54 pKa = 4.56IPDD57 pKa = 3.56EE58 pKa = 4.18EE59 pKa = 4.49AEE61 pKa = 5.03KK62 pKa = 9.69ITTVQAAIDD71 pKa = 4.15FIKK74 pKa = 10.41EE75 pKa = 4.04SKK77 pKa = 10.09KK78 pKa = 10.84

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P60119|REPX_WIGBR Putative replication protein OS=Wigglesworthia glossinidia brevipalpis OX=36870 GN=WIGBRp0010 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.0KK4 pKa = 10.04QNSSISRR11 pKa = 11.84KK12 pKa = 9.35KK13 pKa = 9.8IKK15 pKa = 10.22RR16 pKa = 11.84QILEE20 pKa = 4.71GIAHH24 pKa = 6.47IHH26 pKa = 6.33ASFNNTIVTITDD38 pKa = 3.45RR39 pKa = 11.84QGNTLGWATSGGSGFRR55 pKa = 11.84GSRR58 pKa = 11.84KK59 pKa = 8.26STPFAAQVAAEE70 pKa = 4.26KK71 pKa = 10.49CSEE74 pKa = 3.75IAKK77 pKa = 10.3EE78 pKa = 3.82YY79 pKa = 10.46GIKK82 pKa = 10.41NLEE85 pKa = 4.08IMVKK89 pKa = 10.32GPGPGRR95 pKa = 11.84EE96 pKa = 3.99STIRR100 pKa = 11.84ALNSSGFKK108 pKa = 8.52ITNITDD114 pKa = 3.35VTPIPHH120 pKa = 6.41NGCRR124 pKa = 11.84PPKK127 pKa = 9.63RR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84VV131 pKa = 3.29

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

617

0

617

202882

38

1405

328.8

37.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.638 ± 0.079

1.269 ± 0.029

3.993 ± 0.056

5.11 ± 0.081

5.567 ± 0.094

5.168 ± 0.095

1.624 ± 0.035

13.249 ± 0.126

11.657 ± 0.133

9.455 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.038

8.486 ± 0.116

2.826 ± 0.047

2.278 ± 0.043

3.117 ± 0.066

7.68 ± 0.068

3.703 ± 0.05

4.205 ± 0.071

0.845 ± 0.032

4.111 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski