Amino acid dipepetide frequency for Mycobacterium phage WIVsmall

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.437AlaAla: 16.437 ± 2.577
1.313AlaCys: 1.313 ± 0.335
7.02AlaAsp: 7.02 ± 0.602
7.363AlaGlu: 7.363 ± 0.974
2.854AlaPhe: 2.854 ± 0.433
10.045AlaGly: 10.045 ± 1.29
2.397AlaHis: 2.397 ± 0.523
4.509AlaIle: 4.509 ± 0.602
3.824AlaLys: 3.824 ± 0.601
9.988AlaLeu: 9.988 ± 1.127
2.34AlaMet: 2.34 ± 0.383
3.196AlaAsn: 3.196 ± 0.564
6.906AlaPro: 6.906 ± 0.853
2.511AlaGln: 2.511 ± 0.437
7.477AlaArg: 7.477 ± 0.911
7.534AlaSer: 7.534 ± 1.19
6.449AlaThr: 6.449 ± 0.691
7.648AlaVal: 7.648 ± 0.595
2.454AlaTrp: 2.454 ± 0.458
2.055AlaTyr: 2.055 ± 0.32
0.0AlaXaa: 0.0 ± 0.0
Cys
1.484CysAla: 1.484 ± 0.423
0.285CysCys: 0.285 ± 0.107
1.027CysAsp: 1.027 ± 0.225
0.571CysGlu: 0.571 ± 0.199
0.171CysPhe: 0.171 ± 0.107
1.655CysGly: 1.655 ± 0.354
0.228CysHis: 0.228 ± 0.109
0.457CysIle: 0.457 ± 0.16
0.4CysLys: 0.4 ± 0.16
0.628CysLeu: 0.628 ± 0.19
0.228CysMet: 0.228 ± 0.128
0.4CysAsn: 0.4 ± 0.133
0.97CysPro: 0.97 ± 0.264
0.514CysGln: 0.514 ± 0.184
0.685CysArg: 0.685 ± 0.184
0.97CysSer: 0.97 ± 0.283
1.141CysThr: 1.141 ± 0.282
0.571CysVal: 0.571 ± 0.179
0.4CysTrp: 0.4 ± 0.148
0.285CysTyr: 0.285 ± 0.128
0.0CysXaa: 0.0 ± 0.0
Asp
6.735AspAla: 6.735 ± 0.654
0.799AspCys: 0.799 ± 0.195
4.395AspAsp: 4.395 ± 0.669
3.196AspGlu: 3.196 ± 0.491
1.541AspPhe: 1.541 ± 0.33
5.479AspGly: 5.479 ± 0.587
1.484AspHis: 1.484 ± 0.243
2.968AspIle: 2.968 ± 0.486
1.484AspLys: 1.484 ± 0.235
5.65AspLeu: 5.65 ± 0.558
1.199AspMet: 1.199 ± 0.283
1.427AspAsn: 1.427 ± 0.311
3.767AspPro: 3.767 ± 0.497
2.797AspGln: 2.797 ± 0.374
5.879AspArg: 5.879 ± 0.753
3.367AspSer: 3.367 ± 0.472
3.71AspThr: 3.71 ± 0.499
4.509AspVal: 4.509 ± 0.593
1.199AspTrp: 1.199 ± 0.257
1.541AspTyr: 1.541 ± 0.319
0.0AspXaa: 0.0 ± 0.0
Glu
5.993GluAla: 5.993 ± 0.851
0.913GluCys: 0.913 ± 0.206
3.196GluAsp: 3.196 ± 0.348
2.34GluGlu: 2.34 ± 0.393
1.769GluPhe: 1.769 ± 0.291
2.74GluGly: 2.74 ± 0.454
1.37GluHis: 1.37 ± 0.351
2.625GluIle: 2.625 ± 0.362
1.655GluLys: 1.655 ± 0.283
4.68GluLeu: 4.68 ± 0.667
1.598GluMet: 1.598 ± 0.27
1.883GluAsn: 1.883 ± 0.278
3.367GluPro: 3.367 ± 0.591
2.568GluGln: 2.568 ± 0.405
3.881GluArg: 3.881 ± 0.527
3.424GluSer: 3.424 ± 0.654
3.653GluThr: 3.653 ± 0.541
3.824GluVal: 3.824 ± 0.485
1.027GluTrp: 1.027 ± 0.252
0.856GluTyr: 0.856 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
2.911PheAla: 2.911 ± 0.421
0.457PheCys: 0.457 ± 0.16
2.055PheAsp: 2.055 ± 0.294
1.256PheGlu: 1.256 ± 0.252
0.913PhePhe: 0.913 ± 0.24
2.34PheGly: 2.34 ± 0.511
0.628PheHis: 0.628 ± 0.205
1.084PheIle: 1.084 ± 0.294
1.256PheLys: 1.256 ± 0.294
2.112PheLeu: 2.112 ± 0.315
0.97PheMet: 0.97 ± 0.262
1.084PheAsn: 1.084 ± 0.335
1.655PhePro: 1.655 ± 0.358
0.97PheGln: 0.97 ± 0.322
1.541PheArg: 1.541 ± 0.298
1.655PheSer: 1.655 ± 0.292
2.169PheThr: 2.169 ± 0.345
1.826PheVal: 1.826 ± 0.26
0.4PheTrp: 0.4 ± 0.156
0.685PheTyr: 0.685 ± 0.237
0.0PheXaa: 0.0 ± 0.0
Gly
9.874GlyAla: 9.874 ± 1.439
1.313GlyCys: 1.313 ± 0.309
6.335GlyAsp: 6.335 ± 0.593
4.166GlyGlu: 4.166 ± 0.487
2.226GlyPhe: 2.226 ± 0.388
11.13GlyGly: 11.13 ± 2.111
1.655GlyHis: 1.655 ± 0.274
3.539GlyIle: 3.539 ± 0.445
2.625GlyLys: 2.625 ± 0.312
5.365GlyLeu: 5.365 ± 0.613
1.826GlyMet: 1.826 ± 0.378
2.968GlyAsn: 2.968 ± 0.471
4.224GlyPro: 4.224 ± 0.59
3.082GlyGln: 3.082 ± 0.637
4.908GlyArg: 4.908 ± 0.661
6.678GlySer: 6.678 ± 0.935
5.936GlyThr: 5.936 ± 0.635
6.392GlyVal: 6.392 ± 0.702
1.826GlyTrp: 1.826 ± 0.365
2.283GlyTyr: 2.283 ± 0.411
0.0GlyXaa: 0.0 ± 0.0
His
2.568HisAla: 2.568 ± 0.519
0.4HisCys: 0.4 ± 0.173
1.084HisAsp: 1.084 ± 0.281
1.256HisGlu: 1.256 ± 0.235
0.285HisPhe: 0.285 ± 0.128
1.655HisGly: 1.655 ± 0.305
0.799HisHis: 0.799 ± 0.246
1.598HisIle: 1.598 ± 0.359
0.742HisLys: 0.742 ± 0.253
2.34HisLeu: 2.34 ± 0.374
0.342HisMet: 0.342 ± 0.123
0.856HisAsn: 0.856 ± 0.261
1.37HisPro: 1.37 ± 0.262
0.799HisGln: 0.799 ± 0.217
2.625HisArg: 2.625 ± 0.753
0.913HisSer: 0.913 ± 0.25
1.141HisThr: 1.141 ± 0.313
1.256HisVal: 1.256 ± 0.311
0.4HisTrp: 0.4 ± 0.158
0.742HisTyr: 0.742 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
5.479IleAla: 5.479 ± 0.575
0.742IleCys: 0.742 ± 0.267
3.653IleAsp: 3.653 ± 0.423
2.911IleGlu: 2.911 ± 0.469
0.856IlePhe: 0.856 ± 0.26
4.338IleGly: 4.338 ± 0.492
1.027IleHis: 1.027 ± 0.215
1.484IleIle: 1.484 ± 0.306
1.141IleLys: 1.141 ± 0.306
1.883IleLeu: 1.883 ± 0.326
0.628IleMet: 0.628 ± 0.175
1.655IleAsn: 1.655 ± 0.276
3.367IlePro: 3.367 ± 0.49
1.712IleGln: 1.712 ± 0.275
2.397IleArg: 2.397 ± 0.571
3.025IleSer: 3.025 ± 0.546
3.253IleThr: 3.253 ± 0.373
3.025IleVal: 3.025 ± 0.34
0.628IleTrp: 0.628 ± 0.188
0.799IleTyr: 0.799 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
3.881LysAla: 3.881 ± 0.521
0.457LysCys: 0.457 ± 0.156
1.427LysAsp: 1.427 ± 0.287
1.027LysGlu: 1.027 ± 0.263
1.027LysPhe: 1.027 ± 0.179
1.883LysGly: 1.883 ± 0.369
1.027LysHis: 1.027 ± 0.269
1.313LysIle: 1.313 ± 0.295
1.313LysLys: 1.313 ± 0.302
2.854LysLeu: 2.854 ± 0.444
0.571LysMet: 0.571 ± 0.188
0.97LysAsn: 0.97 ± 0.243
1.941LysPro: 1.941 ± 0.322
0.97LysGln: 0.97 ± 0.202
2.112LysArg: 2.112 ± 0.388
2.511LysSer: 2.511 ± 0.339
1.941LysThr: 1.941 ± 0.335
2.055LysVal: 2.055 ± 0.338
0.856LysTrp: 0.856 ± 0.29
0.799LysTyr: 0.799 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
7.534LeuAla: 7.534 ± 0.687
0.742LeuCys: 0.742 ± 0.274
5.308LeuAsp: 5.308 ± 0.619
3.31LeuGlu: 3.31 ± 0.41
2.226LeuPhe: 2.226 ± 0.304
5.536LeuGly: 5.536 ± 0.63
1.313LeuHis: 1.313 ± 0.456
3.539LeuIle: 3.539 ± 0.478
2.226LeuLys: 2.226 ± 0.357
5.765LeuLeu: 5.765 ± 0.714
1.941LeuMet: 1.941 ± 0.289
2.454LeuAsn: 2.454 ± 0.389
4.737LeuPro: 4.737 ± 0.527
2.854LeuGln: 2.854 ± 0.514
6.392LeuArg: 6.392 ± 0.827
5.936LeuSer: 5.936 ± 0.767
5.194LeuThr: 5.194 ± 0.636
5.365LeuVal: 5.365 ± 0.681
1.141LeuTrp: 1.141 ± 0.262
1.541LeuTyr: 1.541 ± 0.366
0.0LeuXaa: 0.0 ± 0.0
Met
1.712MetAla: 1.712 ± 0.363
0.457MetCys: 0.457 ± 0.2
1.313MetAsp: 1.313 ± 0.298
1.427MetGlu: 1.427 ± 0.319
0.742MetPhe: 0.742 ± 0.209
1.769MetGly: 1.769 ± 0.342
0.228MetHis: 0.228 ± 0.114
0.856MetIle: 0.856 ± 0.226
0.799MetLys: 0.799 ± 0.287
1.769MetLeu: 1.769 ± 0.238
0.514MetMet: 0.514 ± 0.219
0.97MetAsn: 0.97 ± 0.238
1.313MetPro: 1.313 ± 0.264
0.228MetGln: 0.228 ± 0.13
1.541MetArg: 1.541 ± 0.291
1.941MetSer: 1.941 ± 0.303
1.712MetThr: 1.712 ± 0.324
1.655MetVal: 1.655 ± 0.31
0.342MetTrp: 0.342 ± 0.124
0.285MetTyr: 0.285 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
3.367AsnAla: 3.367 ± 0.465
0.514AsnCys: 0.514 ± 0.171
1.712AsnAsp: 1.712 ± 0.287
1.883AsnGlu: 1.883 ± 0.327
0.514AsnPhe: 0.514 ± 0.191
4.68AsnGly: 4.68 ± 0.764
1.199AsnHis: 1.199 ± 0.248
1.141AsnIle: 1.141 ± 0.359
0.97AsnLys: 0.97 ± 0.244
2.797AsnLeu: 2.797 ± 0.365
0.628AsnMet: 0.628 ± 0.163
1.998AsnAsn: 1.998 ± 0.459
2.797AsnPro: 2.797 ± 0.36
0.97AsnGln: 0.97 ± 0.333
2.112AsnArg: 2.112 ± 0.351
1.998AsnSer: 1.998 ± 0.335
1.941AsnThr: 1.941 ± 0.321
2.112AsnVal: 2.112 ± 0.354
0.457AsnTrp: 0.457 ± 0.159
0.514AsnTyr: 0.514 ± 0.146
0.0AsnXaa: 0.0 ± 0.0
Pro
7.02ProAla: 7.02 ± 0.875
0.457ProCys: 0.457 ± 0.149
3.767ProAsp: 3.767 ± 0.476
4.452ProGlu: 4.452 ± 0.615
2.511ProPhe: 2.511 ± 0.383
6.221ProGly: 6.221 ± 0.482
1.655ProHis: 1.655 ± 0.368
2.226ProIle: 2.226 ± 0.268
1.769ProLys: 1.769 ± 0.376
4.224ProLeu: 4.224 ± 0.487
1.141ProMet: 1.141 ± 0.3
2.34ProAsn: 2.34 ± 0.388
3.367ProPro: 3.367 ± 0.525
2.34ProGln: 2.34 ± 0.367
3.31ProArg: 3.31 ± 0.634
3.653ProSer: 3.653 ± 0.526
3.881ProThr: 3.881 ± 0.64
4.851ProVal: 4.851 ± 0.586
0.742ProTrp: 0.742 ± 0.23
1.256ProTyr: 1.256 ± 0.348
0.0ProXaa: 0.0 ± 0.0
Gln
3.881GlnAla: 3.881 ± 0.552
0.228GlnCys: 0.228 ± 0.14
1.256GlnAsp: 1.256 ± 0.317
1.541GlnGlu: 1.541 ± 0.292
1.427GlnPhe: 1.427 ± 0.291
2.454GlnGly: 2.454 ± 0.502
0.742GlnHis: 0.742 ± 0.201
1.883GlnIle: 1.883 ± 0.35
1.199GlnLys: 1.199 ± 0.257
3.424GlnLeu: 3.424 ± 0.627
0.628GlnMet: 0.628 ± 0.198
1.027GlnAsn: 1.027 ± 0.225
2.568GlnPro: 2.568 ± 0.513
1.769GlnGln: 1.769 ± 0.447
2.34GlnArg: 2.34 ± 0.468
1.883GlnSer: 1.883 ± 0.393
1.427GlnThr: 1.427 ± 0.302
2.511GlnVal: 2.511 ± 0.408
0.685GlnTrp: 0.685 ± 0.226
1.313GlnTyr: 1.313 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
6.735ArgAla: 6.735 ± 1.041
1.712ArgCys: 1.712 ± 0.473
4.68ArgAsp: 4.68 ± 0.606
4.052ArgGlu: 4.052 ± 0.487
2.397ArgPhe: 2.397 ± 0.459
4.566ArgGly: 4.566 ± 0.556
1.883ArgHis: 1.883 ± 0.885
4.052ArgIle: 4.052 ± 0.467
2.454ArgLys: 2.454 ± 0.393
4.794ArgLeu: 4.794 ± 0.576
1.826ArgMet: 1.826 ± 0.337
2.74ArgAsn: 2.74 ± 0.516
3.539ArgPro: 3.539 ± 0.55
2.568ArgGln: 2.568 ± 0.737
6.107ArgArg: 6.107 ± 1.387
4.166ArgSer: 4.166 ± 0.615
3.253ArgThr: 3.253 ± 0.662
5.194ArgVal: 5.194 ± 0.742
1.37ArgTrp: 1.37 ± 0.333
1.826ArgTyr: 1.826 ± 0.302
0.0ArgXaa: 0.0 ± 0.0
Ser
9.132SerAla: 9.132 ± 1.585
0.514SerCys: 0.514 ± 0.224
3.139SerAsp: 3.139 ± 0.486
3.196SerGlu: 3.196 ± 0.411
1.883SerPhe: 1.883 ± 0.31
7.591SerGly: 7.591 ± 0.985
1.598SerHis: 1.598 ± 0.296
2.797SerIle: 2.797 ± 0.372
1.998SerLys: 1.998 ± 0.345
3.539SerLeu: 3.539 ± 0.4
1.941SerMet: 1.941 ± 0.325
2.055SerAsn: 2.055 ± 0.333
3.767SerPro: 3.767 ± 0.423
1.37SerGln: 1.37 ± 0.255
4.395SerArg: 4.395 ± 0.652
4.908SerSer: 4.908 ± 0.765
4.452SerThr: 4.452 ± 0.803
4.68SerVal: 4.68 ± 0.472
1.256SerTrp: 1.256 ± 0.23
1.484SerTyr: 1.484 ± 0.251
0.0SerXaa: 0.0 ± 0.0
Thr
7.876ThrAla: 7.876 ± 0.773
0.571ThrCys: 0.571 ± 0.14
3.938ThrAsp: 3.938 ± 0.7
3.482ThrGlu: 3.482 ± 0.463
1.655ThrPhe: 1.655 ± 0.32
5.536ThrGly: 5.536 ± 0.588
1.484ThrHis: 1.484 ± 0.305
3.31ThrIle: 3.31 ± 0.475
1.769ThrLys: 1.769 ± 0.293
3.881ThrLeu: 3.881 ± 0.455
0.799ThrMet: 0.799 ± 0.235
2.169ThrAsn: 2.169 ± 0.334
4.68ThrPro: 4.68 ± 0.652
2.055ThrGln: 2.055 ± 0.316
4.509ThrArg: 4.509 ± 0.827
4.052ThrSer: 4.052 ± 0.492
5.308ThrThr: 5.308 ± 0.654
6.107ThrVal: 6.107 ± 0.637
0.685ThrTrp: 0.685 ± 0.194
1.655ThrTyr: 1.655 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
8.105ValAla: 8.105 ± 0.751
0.856ValCys: 0.856 ± 0.223
5.137ValAsp: 5.137 ± 0.543
3.367ValGlu: 3.367 ± 0.458
1.598ValPhe: 1.598 ± 0.35
5.593ValGly: 5.593 ± 0.682
1.655ValHis: 1.655 ± 0.319
2.74ValIle: 2.74 ± 0.419
2.169ValLys: 2.169 ± 0.384
5.993ValLeu: 5.993 ± 0.974
1.484ValMet: 1.484 ± 0.282
2.74ValAsn: 2.74 ± 0.376
4.851ValPro: 4.851 ± 0.582
2.511ValGln: 2.511 ± 0.331
5.137ValArg: 5.137 ± 1.019
4.794ValSer: 4.794 ± 0.6
5.822ValThr: 5.822 ± 0.555
6.792ValVal: 6.792 ± 0.636
1.313ValTrp: 1.313 ± 0.293
1.199ValTyr: 1.199 ± 0.283
0.0ValXaa: 0.0 ± 0.0
Trp
1.484TrpAla: 1.484 ± 0.242
0.171TrpCys: 0.171 ± 0.093
0.97TrpAsp: 0.97 ± 0.223
0.97TrpGlu: 0.97 ± 0.236
0.685TrpPhe: 0.685 ± 0.249
1.084TrpGly: 1.084 ± 0.278
0.514TrpHis: 0.514 ± 0.158
0.97TrpIle: 0.97 ± 0.211
0.457TrpLys: 0.457 ± 0.169
1.313TrpLeu: 1.313 ± 0.321
0.685TrpMet: 0.685 ± 0.203
0.571TrpAsn: 0.571 ± 0.19
0.913TrpPro: 0.913 ± 0.187
1.027TrpGln: 1.027 ± 0.273
1.427TrpArg: 1.427 ± 0.28
1.484TrpSer: 1.484 ± 0.331
1.313TrpThr: 1.313 ± 0.315
1.084TrpVal: 1.084 ± 0.288
0.742TrpTrp: 0.742 ± 0.211
0.457TrpTyr: 0.457 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.112TyrAla: 2.112 ± 0.381
0.171TyrCys: 0.171 ± 0.102
1.712TyrAsp: 1.712 ± 0.373
1.598TyrGlu: 1.598 ± 0.323
0.685TyrPhe: 0.685 ± 0.221
2.055TyrGly: 2.055 ± 0.352
0.285TyrHis: 0.285 ± 0.116
0.913TyrIle: 0.913 ± 0.191
0.685TyrLys: 0.685 ± 0.219
2.055TyrLeu: 2.055 ± 0.382
0.228TyrMet: 0.228 ± 0.109
0.799TyrAsn: 0.799 ± 0.202
1.084TyrPro: 1.084 ± 0.246
0.571TyrGln: 0.571 ± 0.148
1.199TyrArg: 1.199 ± 0.274
0.799TyrSer: 0.799 ± 0.219
1.826TyrThr: 1.826 ± 0.405
2.397TyrVal: 2.397 ± 0.411
0.4TyrTrp: 0.4 ± 0.131
0.514TyrTyr: 0.514 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (17522 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski