Candidatus Neoehrlichia lotoris str. RAC413

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Candidatus Neoehrlichia; Candidatus Neoehrlichia lotoris

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1017 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F3NM67|A0A0F3NM67_9RICK Uncharacterized protein OS=Candidatus Neoehrlichia lotoris str. RAC413 OX=1359163 GN=NLO413_0162 PE=4 SV=1
MM1 pKa = 7.47SFQVQEE7 pKa = 3.87FVYY10 pKa = 10.87SIFAEE15 pKa = 4.8GKK17 pKa = 8.4LCSVEE22 pKa = 3.82YY23 pKa = 10.75DD24 pKa = 3.84SIFGDD29 pKa = 3.7DD30 pKa = 3.96VKK32 pKa = 11.58NEE34 pKa = 4.69FIPCEE39 pKa = 3.84

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F3NLU8|A0A0F3NLU8_9RICK Uncharacterized protein OS=Candidatus Neoehrlichia lotoris str. RAC413 OX=1359163 GN=NLO413_0109 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IVRR12 pKa = 11.84KK13 pKa = 9.02RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.86GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 9.24WGRR28 pKa = 11.84KK29 pKa = 6.7ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84MQGRR39 pKa = 11.84RR40 pKa = 11.84ILCAA44 pKa = 3.65

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1017

0

1017

310528

37

5117

305.3

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.212 ± 0.069

1.605 ± 0.037

5.262 ± 0.19

4.988 ± 0.1

4.528 ± 0.084

5.184 ± 0.093

2.286 ± 0.04

10.707 ± 0.141

7.861 ± 0.074

9.406 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.035

7.412 ± 0.12

2.797 ± 0.046

3.281 ± 0.058

3.19 ± 0.052

7.728 ± 0.065

4.839 ± 0.066

6.35 ± 0.082

0.661 ± 0.024

4.341 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski