Circoviridae 21 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNG1|S5TNG1_9CIRC Uncharacterized protein OS=Circoviridae 21 LDMD-2013 OX=1379725 PE=4 SV=1
MM1 pKa = 7.63PRR3 pKa = 11.84TRR5 pKa = 11.84TTTPEE10 pKa = 3.43TNMRR14 pKa = 11.84RR15 pKa = 11.84NWCITVNNPVEE26 pKa = 5.34DD27 pKa = 4.13PEE29 pKa = 4.53TWSKK33 pKa = 11.04RR34 pKa = 11.84LRR36 pKa = 11.84THH38 pKa = 6.62SLFKK42 pKa = 11.01YY43 pKa = 10.41LVFQAEE49 pKa = 4.47KK50 pKa = 9.95GDD52 pKa = 3.64SGTIHH57 pKa = 5.56YY58 pKa = 8.96QAYY61 pKa = 10.47AEE63 pKa = 4.1FSNPIRR69 pKa = 11.84FGSVKK74 pKa = 10.31YY75 pKa = 9.5LIPGAHH81 pKa = 6.0IEE83 pKa = 3.96ARR85 pKa = 11.84RR86 pKa = 11.84GSAKK90 pKa = 9.88QASDD94 pKa = 3.54YY95 pKa = 10.82CKK97 pKa = 10.32KK98 pKa = 10.63EE99 pKa = 4.06KK100 pKa = 10.19GRR102 pKa = 11.84KK103 pKa = 8.14AGPWEE108 pKa = 3.88YY109 pKa = 11.53GEE111 pKa = 5.27LSVEE115 pKa = 4.12RR116 pKa = 11.84QGQRR120 pKa = 11.84LDD122 pKa = 3.69LEE124 pKa = 4.29RR125 pKa = 11.84LAQIAQTHH133 pKa = 4.97SHH135 pKa = 5.69TDD137 pKa = 2.84ILKK140 pKa = 9.83EE141 pKa = 3.94MPRR144 pKa = 11.84EE145 pKa = 3.58YY146 pKa = 10.91AKK148 pKa = 10.66YY149 pKa = 8.97YY150 pKa = 10.9KK151 pKa = 10.19FVQHH155 pKa = 5.98VKK157 pKa = 10.34HH158 pKa = 6.79IEE160 pKa = 3.95AMDD163 pKa = 3.63KK164 pKa = 10.83PGIRR168 pKa = 11.84SNLQVIVCHH177 pKa = 6.52GEE179 pKa = 3.66TRR181 pKa = 11.84TGKK184 pKa = 6.83THH186 pKa = 5.91YY187 pKa = 10.46AYY189 pKa = 10.13EE190 pKa = 4.78RR191 pKa = 11.84DD192 pKa = 3.8PNLYY196 pKa = 10.13ALPVGKK202 pKa = 9.82DD203 pKa = 3.13LWFDD207 pKa = 3.43NYY209 pKa = 11.03NGEE212 pKa = 4.32KK213 pKa = 9.63TVLLDD218 pKa = 5.06DD219 pKa = 5.34FSGQCRR225 pKa = 11.84LVDD228 pKa = 4.4LLRR231 pKa = 11.84ILDD234 pKa = 4.29KK235 pKa = 11.45YY236 pKa = 9.94PVQIPRR242 pKa = 11.84KK243 pKa = 10.11GGFVWLKK250 pKa = 10.47AEE252 pKa = 4.53TIIITCNQHH261 pKa = 6.01PEE263 pKa = 3.41TWYY266 pKa = 10.28DD267 pKa = 3.32YY268 pKa = 11.46SNRR271 pKa = 11.84DD272 pKa = 3.59SLRR275 pKa = 11.84KK276 pKa = 9.77ALMARR281 pKa = 11.84FTRR284 pKa = 11.84FLEE287 pKa = 4.69FTAGGRR293 pKa = 11.84KK294 pKa = 9.48AIQLGGDD301 pKa = 3.28HH302 pKa = 6.55TRR304 pKa = 11.84PANEE308 pKa = 3.63IVEE311 pKa = 4.28ARR313 pKa = 11.84RR314 pKa = 11.84DD315 pKa = 3.57STTPIPEE322 pKa = 4.39TPPQSPVRR330 pKa = 11.84RR331 pKa = 11.84DD332 pKa = 3.0SDD334 pKa = 3.69VSLTPTIEE342 pKa = 4.06LSTIEE347 pKa = 4.85DD348 pKa = 4.25DD349 pKa = 5.16YY350 pKa = 11.88IVIDD354 pKa = 4.23DD355 pKa = 3.91

Molecular weight: 41.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TMY9|S5TMY9_9CIRC ATP-dependent helicase Rep OS=Circoviridae 21 LDMD-2013 OX=1379725 PE=3 SV=1
MM1 pKa = 7.83AYY3 pKa = 10.22NRR5 pKa = 11.84RR6 pKa = 11.84TFEE9 pKa = 3.93MVVNPRR15 pKa = 11.84PYY17 pKa = 10.11RR18 pKa = 11.84KK19 pKa = 9.42RR20 pKa = 11.84GRR22 pKa = 11.84SWNAPVGGVYY32 pKa = 10.14KK33 pKa = 10.5KK34 pKa = 10.58RR35 pKa = 11.84RR36 pKa = 11.84IASAQKK42 pKa = 10.12GYY44 pKa = 10.97VRR46 pKa = 11.84TTGYY50 pKa = 10.35YY51 pKa = 10.59GRR53 pKa = 11.84FSGKK57 pKa = 8.87PGEE60 pKa = 4.29MKK62 pKa = 10.69FFDD65 pKa = 3.7TDD67 pKa = 3.27KK68 pKa = 11.13TGTTVAAAGTVLNSSLLLIPDD89 pKa = 4.11GTTQSTRR96 pKa = 11.84IGRR99 pKa = 11.84KK100 pKa = 7.62ITVKK104 pKa = 10.22HH105 pKa = 5.79IFVHH109 pKa = 5.74GHH111 pKa = 7.27LILPATTTPGDD122 pKa = 3.26TSDD125 pKa = 3.17RR126 pKa = 11.84VRR128 pKa = 11.84IIMYY132 pKa = 10.12LDD134 pKa = 3.52KK135 pKa = 10.99QCNGAAAAVSDD146 pKa = 4.26ILTATDD152 pKa = 3.35VNSFRR157 pKa = 11.84NLSEE161 pKa = 3.7TGRR164 pKa = 11.84FHH166 pKa = 7.83ILYY169 pKa = 10.49DD170 pKa = 3.75KK171 pKa = 9.73VHH173 pKa = 6.29SMNASSGTTAGAGGDD188 pKa = 3.42QFGEE192 pKa = 4.19YY193 pKa = 9.17MRR195 pKa = 11.84GFSIKK200 pKa = 9.85KK201 pKa = 8.02ACNYY205 pKa = 9.23PIEE208 pKa = 4.37YY209 pKa = 10.47NNTTGAIGEE218 pKa = 4.19IRR220 pKa = 11.84SNNXGIXAISASGHH234 pKa = 4.96CTATYY239 pKa = 9.06KK240 pKa = 10.93ARR242 pKa = 11.84VRR244 pKa = 11.84YY245 pKa = 9.59LDD247 pKa = 3.17QQ248 pKa = 5.66

Molecular weight: 26.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

719

116

355

239.7

26.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.232 ± 0.936

1.808 ± 0.515

5.007 ± 1.033

4.59 ± 1.661

3.477 ± 0.265

7.093 ± 1.428

2.503 ± 0.442

6.12 ± 0.196

5.702 ± 0.37

7.093 ± 1.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.592

4.033 ± 0.554

5.563 ± 1.019

2.643 ± 0.899

7.65 ± 0.738

6.676 ± 1.203

8.206 ± 0.787

6.676 ± 1.547

1.113 ± 0.409

4.312 ± 0.706
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski