Bovine foamy virus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J9VDW6|J9VDW6_9RETR Tas OS=Bovine foamy virus OX=207343 GN=bel1 PE=4 SV=1
MM1 pKa = 7.7 ASGGTPEE8 pKa = 4.3 KK9 pKa = 10.96 ARR11 pKa = 11.84 VACRR15 pKa = 11.84 RR16 pKa = 11.84 VDD18 pKa = 3.69 LSSFLAQPDD27 pKa = 4.52 DD28 pKa = 4.08 YY29 pKa = 10.64 PTAADD34 pKa = 3.73 SKK36 pKa = 11.1 EE37 pKa = 3.96 DD38 pKa = 4.39 LILKK42 pKa = 7.48 LACTTLFSEE51 pKa = 4.5 KK52 pKa = 9.52 HH53 pKa = 4.38 AHH55 pKa = 6.78 EE56 pKa = 4.5 IYY58 pKa = 10.69 EE59 pKa = 4.1 NYY61 pKa = 9.97 KK62 pKa = 10.36 LHH64 pKa = 7.14 LKK66 pKa = 10.13 RR67 pKa = 11.84 DD68 pKa = 3.63 EE69 pKa = 4.08 LRR71 pKa = 11.84 GGKK74 pKa = 8.61 EE75 pKa = 3.19 WVIIYY80 pKa = 9.72 SCKK83 pKa = 9.4 HH84 pKa = 6.16 CYY86 pKa = 7.86 TVFMDD91 pKa = 4.06 NSRR94 pKa = 11.84 LTLDD98 pKa = 3.18 PSGLFRR104 pKa = 11.84 VIRR107 pKa = 11.84 NKK109 pKa = 10.24 KK110 pKa = 8.51 GPYY113 pKa = 8.72 MLCQMLTRR121 pKa = 11.84 HH122 pKa = 5.4 LTDD125 pKa = 2.96 RR126 pKa = 11.84 CNPRR130 pKa = 11.84 TKK132 pKa = 10.14 PFQSSSSLHH141 pKa = 6.27 PNLVTEE147 pKa = 4.86 DD148 pKa = 3.51 PRR150 pKa = 11.84 GAGGGTPGQHH160 pKa = 5.79 TLGGDD165 pKa = 2.66 QDD167 pKa = 3.8 MRR169 pKa = 11.84 VNTSGIKK176 pKa = 9.71 PLSSLCQCARR186 pKa = 11.84 DD187 pKa = 3.97 DD188 pKa = 4.73 PGRR191 pKa = 11.84 SDD193 pKa = 4.31 NPLEE197 pKa = 4.12 MAEE200 pKa = 4.6 PVQPWWTDD208 pKa = 2.79 SSLEE212 pKa = 4.03 PEE214 pKa = 3.72 IATWVLGNPDD224 pKa = 2.66 ATARR228 pKa = 11.84 FWTGDD233 pKa = 3.4 DD234 pKa = 4.47 KK235 pKa = 11.78 GPQEE239 pKa = 4.33 WDD241 pKa = 3.27 FDD243 pKa = 4.84 DD244 pKa = 6.28 DD245 pKa = 4.96 LLGPP249 pKa = 4.64
Molecular weight: 27.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.328
IPC2_protein 5.334
IPC_protein 5.308
Toseland 5.461
ProMoST 5.563
Dawson 5.436
Bjellqvist 5.512
Wikipedia 5.397
Rodwell 5.372
Grimsley 5.486
Solomon 5.436
Lehninger 5.41
Nozaki 5.639
DTASelect 5.842
Thurlkill 5.626
EMBOSS 5.614
Sillero 5.715
Patrickios 4.088
IPC_peptide 5.448
IPC2_peptide 5.715
IPC2.peptide.svr19 5.724
Protein with the highest isoelectric point:
>tr|J9V9W7|J9V9W7_9RETR Bet OS=Bovine foamy virus OX=207343 GN=bel2 PE=4 SV=1
MM1 pKa = 7.76 ALNDD5 pKa = 4.11 FDD7 pKa = 6.17 PIALQGYY14 pKa = 8.63 LPAPRR19 pKa = 11.84 VLQHH23 pKa = 6.42 NDD25 pKa = 2.94 IIICRR30 pKa = 11.84 ATSGPWGIGDD40 pKa = 4.22 RR41 pKa = 11.84 YY42 pKa = 10.43 NLIRR46 pKa = 11.84 IHH48 pKa = 6.27 LQDD51 pKa = 4.15 PAGQPLPIPQWEE63 pKa = 4.51 PIPNRR68 pKa = 11.84 TANPRR73 pKa = 11.84 TQPYY77 pKa = 8.94 PVVSAPMATLEE88 pKa = 4.33 NILNNFHH95 pKa = 6.77 IPHH98 pKa = 6.13 GVSRR102 pKa = 11.84 YY103 pKa = 9.83 GPLEE107 pKa = 4.3 GGDD110 pKa = 3.88 YY111 pKa = 10.88 QPGEE115 pKa = 4.16 QYY117 pKa = 11.05 SQGFCPVTQAEE128 pKa = 4.26 IALLNGQHH136 pKa = 6.51 LEE138 pKa = 4.08 EE139 pKa = 5.54 EE140 pKa = 4.21 ITILRR145 pKa = 11.84 EE146 pKa = 3.5 ITHH149 pKa = 7.18 RR150 pKa = 11.84 LMQGVRR156 pKa = 11.84 PPAAPQGPAPPPPPAQPPAPLPAPPVGPPPPAVPAPAPGPMPVPQHH202 pKa = 6.46 LPITHH207 pKa = 6.16 IRR209 pKa = 11.84 AVIGEE214 pKa = 4.3 TPANIRR220 pKa = 11.84 EE221 pKa = 4.21 VPLWLARR228 pKa = 11.84 AVPALQGVYY237 pKa = 9.48 PVQDD241 pKa = 2.69 AVMRR245 pKa = 11.84 SRR247 pKa = 11.84 TVNALTVRR255 pKa = 11.84 HH256 pKa = 6.6 PGLALEE262 pKa = 4.36 PLEE265 pKa = 4.46 CGSWQEE271 pKa = 4.11 CLAALWQRR279 pKa = 11.84 TFGATALHH287 pKa = 6.59 ALGDD291 pKa = 4.0 TLGQIANSDD300 pKa = 4.32 GIVMAIEE307 pKa = 4.45 LGLLFSDD314 pKa = 5.57 DD315 pKa = 3.29 NWDD318 pKa = 4.02 LVWGICRR325 pKa = 11.84 RR326 pKa = 11.84 FLPGQAVCVAVQARR340 pKa = 11.84 LDD342 pKa = 4.08 PLPDD346 pKa = 3.22 NATRR350 pKa = 11.84 IVMISHH356 pKa = 8.19 IIRR359 pKa = 11.84 DD360 pKa = 3.64 VYY362 pKa = 10.98 AILGLDD368 pKa = 3.36 PLGRR372 pKa = 11.84 PMQQTLPRR380 pKa = 11.84 RR381 pKa = 11.84 NNQPPRR387 pKa = 11.84 QQPQRR392 pKa = 11.84 RR393 pKa = 11.84 QQPRR397 pKa = 11.84 RR398 pKa = 11.84 TGNQEE403 pKa = 3.16 EE404 pKa = 4.05 RR405 pKa = 11.84 GQRR408 pKa = 11.84 NRR410 pKa = 11.84 GRR412 pKa = 11.84 QNAQTPRR419 pKa = 11.84 QEE421 pKa = 4.19 GNRR424 pKa = 11.84 LQNSQLPGPQDD435 pKa = 3.44 YY436 pKa = 10.0 PNNSNQPRR444 pKa = 11.84 YY445 pKa = 8.98 PLRR448 pKa = 11.84 PNPQQPQRR456 pKa = 11.84 YY457 pKa = 8.09 GQEE460 pKa = 3.91 QNRR463 pKa = 11.84 GNNPNPYY470 pKa = 9.58 RR471 pKa = 11.84 QPTPGNGNQNRR482 pKa = 11.84 NFSRR486 pKa = 11.84 GPAPVNEE493 pKa = 4.26 QSRR496 pKa = 11.84 GRR498 pKa = 11.84 GRR500 pKa = 11.84 NSQGTNNTGSSAVHH514 pKa = 5.3 SVRR517 pKa = 11.84 LTSAAPPIPPQDD529 pKa = 3.61 AGTPPTPSGNQGQSSS544 pKa = 3.44
Molecular weight: 59.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.897
IPC2_protein 8.96
IPC_protein 9.999
Toseland 9.004
ProMoST 9.268
Dawson 9.472
Bjellqvist 9.545
Wikipedia 9.78
Rodwell 9.399
Grimsley 9.575
Solomon 9.575
Lehninger 9.502
Nozaki 9.458
DTASelect 9.428
Thurlkill 9.326
EMBOSS 9.531
Sillero 9.56
Patrickios 4.724
IPC_peptide 9.545
IPC2_peptide 9.136
IPC2.peptide.svr19 8.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3338
249
1220
667.6
75.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.201 ± 0.746
2.067 ± 0.279
5.153 ± 0.497
5.362 ± 0.523
2.337 ± 0.283
5.992 ± 0.781
2.966 ± 0.23
5.752 ± 0.528
4.823 ± 1.258
9.976 ± 0.882
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.588 ± 0.278
4.643 ± 0.515
8.328 ± 1.78
5.812 ± 0.838
5.692 ± 0.834
5.932 ± 0.691
6.171 ± 0.489
5.213 ± 0.436
2.397 ± 0.331
3.595 ± 0.519
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here