Tacheng Tick Virus 7
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5KXG9|A0A0B5KXG9_9RHAB GDP polyribonucleotidyltransferase OS=Tacheng Tick Virus 7 OX=1608089 GN=L PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 5.87 PSQKK6 pKa = 9.38 ATQILGAFRR15 pKa = 11.84 AGQADD20 pKa = 3.91 TTVTATGEE28 pKa = 4.31 SKK30 pKa = 10.05 QWEE33 pKa = 4.68 DD34 pKa = 4.14 KK35 pKa = 10.8 EE36 pKa = 4.69 LDD38 pKa = 4.56 AIPVTPLIALNEE50 pKa = 4.18 AQLSQLFDD58 pKa = 3.69 YY59 pKa = 11.11 AVGEE63 pKa = 4.59 LISPVPVNLPEE74 pKa = 4.49 AVLLLASHH82 pKa = 6.05 VTNPTSRR89 pKa = 11.84 VDD91 pKa = 3.87 DD92 pKa = 3.86 NLLFILKK99 pKa = 9.8 KK100 pKa = 10.0 HH101 pKa = 5.87 LLSKK105 pKa = 10.5 EE106 pKa = 3.87 LAPEE110 pKa = 4.19 LNAIAEE116 pKa = 4.38 KK117 pKa = 10.67 LISTFKK123 pKa = 11.05 AKK125 pKa = 10.07 TGDD128 pKa = 3.42 RR129 pKa = 11.84 TTAAPPDD136 pKa = 3.58 EE137 pKa = 4.47 EE138 pKa = 3.97 AVKK141 pKa = 10.51 RR142 pKa = 11.84 QEE144 pKa = 3.98 QMLAAYY150 pKa = 8.63 YY151 pKa = 9.65 ISKK154 pKa = 8.49 STAVTKK160 pKa = 10.75 DD161 pKa = 3.02 SLGPYY166 pKa = 7.66 MKK168 pKa = 9.2 WYY170 pKa = 10.49 SDD172 pKa = 3.14 ASVADD177 pKa = 3.88 RR178 pKa = 11.84 VEE180 pKa = 5.02 FGCFMSFCLMRR191 pKa = 11.84 SVASQEE197 pKa = 3.7 VTIIRR202 pKa = 11.84 NMGQEE207 pKa = 3.88 IQPTGGSGGTTKK219 pKa = 10.72 GGFYY223 pKa = 10.73 GRR225 pKa = 11.84 YY226 pKa = 7.62 RR227 pKa = 11.84 DD228 pKa = 3.88 LYY230 pKa = 10.24 RR231 pKa = 11.84 GRR233 pKa = 11.84 TFPSCGIQFVTQAAIAQIKK252 pKa = 9.61 AAFSTGSQMLCSILVPVLRR271 pKa = 11.84 VHH273 pKa = 6.65 IQTDD277 pKa = 3.64 PTPSVDD283 pKa = 3.27 VQAVLRR289 pKa = 11.84 YY290 pKa = 9.06 GILMSLEE297 pKa = 3.77 YY298 pKa = 10.3 TGIYY302 pKa = 9.83 LADD305 pKa = 3.66 SYY307 pKa = 11.72 LRR309 pKa = 11.84 ALVRR313 pKa = 11.84 TGEE316 pKa = 4.41 TGSSLIPKK324 pKa = 8.95 LYY326 pKa = 9.85 WNVHH330 pKa = 4.63 HH331 pKa = 7.32 PEE333 pKa = 3.94 YY334 pKa = 10.75 EE335 pKa = 3.94 RR336 pKa = 11.84 LLDD339 pKa = 3.7 MVQKK343 pKa = 10.58 YY344 pKa = 7.62 MRR346 pKa = 11.84 DD347 pKa = 3.56 NKK349 pKa = 10.33 NARR352 pKa = 11.84 AWRR355 pKa = 11.84 WAKK358 pKa = 10.26 CLHH361 pKa = 6.3 SDD363 pKa = 3.67 YY364 pKa = 11.19 LSIYY368 pKa = 10.42 SGVNNPWLCSLFTALFIDD386 pKa = 5.47 KK387 pKa = 9.83 EE388 pKa = 4.27 PQPEE392 pKa = 4.33 GTDD395 pKa = 3.12 VWEE398 pKa = 4.4 KK399 pKa = 10.18 FCLRR403 pKa = 11.84 KK404 pKa = 10.14 SPEE407 pKa = 3.97 HH408 pKa = 6.72 KK409 pKa = 10.14 EE410 pKa = 3.8 WAQKK414 pKa = 9.94 LAAAYY419 pKa = 8.13 WAEE422 pKa = 4.16 KK423 pKa = 9.62 YY424 pKa = 10.3 KK425 pKa = 11.15 NSAITEE431 pKa = 4.16 DD432 pKa = 3.41 SDD434 pKa = 3.91 LVKK437 pKa = 10.64 RR438 pKa = 11.84 IVGVAKK444 pKa = 9.88 PVHH447 pKa = 6.68 DD448 pKa = 5.19 PYY450 pKa = 11.67 DD451 pKa = 3.27 VALRR455 pKa = 11.84 MGSAEE460 pKa = 3.88
Molecular weight: 51.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.042
IPC2_protein 6.109
IPC_protein 6.186
Toseland 6.415
ProMoST 6.376
Dawson 6.504
Bjellqvist 6.478
Wikipedia 6.517
Rodwell 6.491
Grimsley 6.532
Solomon 6.504
Lehninger 6.504
Nozaki 6.766
DTASelect 6.927
Thurlkill 6.956
EMBOSS 6.927
Sillero 6.883
Patrickios 4.113
IPC_peptide 6.517
IPC2_peptide 6.693
IPC2.peptide.svr19 6.754
Protein with the highest isoelectric point:
>tr|A0A0B5KRJ9|A0A0B5KRJ9_9RHAB ORF4 OS=Tacheng Tick Virus 7 OX=1608089 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.48 SKK3 pKa = 10.23 HH4 pKa = 5.98 PKK6 pKa = 9.93 CNPTLYY12 pKa = 10.74 LSIEE16 pKa = 4.41 GKK18 pKa = 10.33 VEE20 pKa = 3.54 VHH22 pKa = 5.27 TLVRR26 pKa = 11.84 PVLDD30 pKa = 4.32 KK31 pKa = 10.98 IVGRR35 pKa = 11.84 LLITSGCPSSGDD47 pKa = 3.47 LKK49 pKa = 10.25 HH50 pKa = 6.4 HH51 pKa = 5.58 VRR53 pKa = 11.84 AAIAGIIHH61 pKa = 7.06 MLTFDD66 pKa = 3.99 PVRR69 pKa = 11.84 GRR71 pKa = 11.84 HH72 pKa = 6.3 LLMTDD77 pKa = 3.53 GGPRR81 pKa = 11.84 MGRR84 pKa = 11.84 QLIWNMSASLIYY96 pKa = 10.88 KK97 pKa = 6.33 MTRR100 pKa = 11.84 DD101 pKa = 3.52 PRR103 pKa = 11.84 VAQLPTFEE111 pKa = 4.47 WSPHH115 pKa = 4.1 GTFSSEE121 pKa = 4.22 DD122 pKa = 3.36 GTVTKK127 pKa = 9.67 WQVHH131 pKa = 5.05 VKK133 pKa = 9.64 TSTAPPSLAALNIPPGRR150 pKa = 11.84 ITDD153 pKa = 3.66 QEE155 pKa = 4.18 YY156 pKa = 8.71 MRR158 pKa = 11.84 ARR160 pKa = 11.84 SLVARR165 pKa = 11.84 FHH167 pKa = 6.39 PPEE170 pKa = 4.52 GSRR173 pKa = 11.84 EE174 pKa = 4.06 PTSPRR179 pKa = 11.84 GDD181 pKa = 3.26 TSGSSQGEE189 pKa = 4.38 EE190 pKa = 4.01 EE191 pKa = 3.92 EE192 pKa = 4.31 HH193 pKa = 7.66 LYY195 pKa = 10.27 EE196 pKa = 4.8 EE197 pKa = 4.87 PMDD200 pKa = 4.1 FLTDD204 pKa = 4.23 LGGLKK209 pKa = 9.21 QRR211 pKa = 11.84 RR212 pKa = 3.68
Molecular weight: 23.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.265
IPC2_protein 8.126
IPC_protein 8.244
Toseland 8.682
ProMoST 8.887
Dawson 8.946
Bjellqvist 8.946
Wikipedia 9.004
Rodwell 9.033
Grimsley 8.653
Solomon 9.224
Lehninger 9.209
Nozaki 9.121
DTASelect 8.829
Thurlkill 8.931
EMBOSS 9.151
Sillero 9.136
Patrickios 5.041
IPC_peptide 9.209
IPC2_peptide 7.819
IPC2.peptide.svr19 7.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4409
212
2215
734.8
82.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.555 ± 0.431
1.86 ± 0.249
4.854 ± 0.449
4.922 ± 0.381
3.357 ± 0.157
5.648 ± 0.213
3.402 ± 0.401
5.466 ± 0.361
4.967 ± 0.365
11.272 ± 0.553
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.949 ± 0.136
3.266 ± 0.169
6.056 ± 0.691
3.946 ± 0.331
5.262 ± 0.282
8.868 ± 0.515
6.305 ± 0.099
6.033 ± 0.319
1.814 ± 0.039
3.198 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here