Poplar mosaic virus
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6RBY7|Q6RBY7_9VIRU Movement protein TGB2 OS=Poplar mosaic virus OX=12166 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.6 WSDD4 pKa = 3.49 SLVSRR9 pKa = 11.84 VCVPIIVVCTSIALLNVVSFRR30 pKa = 11.84 SEE32 pKa = 4.16 CSCVVHH38 pKa = 5.67 ITGEE42 pKa = 4.23 SIDD45 pKa = 3.46 IRR47 pKa = 11.84 GCSFTPDD54 pKa = 4.11 FIEE57 pKa = 4.11 YY58 pKa = 10.54 AKK60 pKa = 9.58 TLRR63 pKa = 11.84 VFNHH67 pKa = 7.3 RR68 pKa = 11.84 YY69 pKa = 9.19 QEE71 pKa = 3.91
Molecular weight: 8.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.431
IPC2_protein 5.792
IPC_protein 5.753
Toseland 5.944
ProMoST 5.537
Dawson 6.046
Bjellqvist 6.046
Wikipedia 6.059
Rodwell 6.033
Grimsley 5.982
Solomon 6.059
Lehninger 6.046
Nozaki 6.3
DTASelect 6.478
Thurlkill 6.504
EMBOSS 6.478
Sillero 6.427
Patrickios 2.13
IPC_peptide 6.071
IPC2_peptide 6.44
IPC2.peptide.svr19 6.445
Protein with the highest isoelectric point:
>tr|Q6RBY8|Q6RBY8_9VIRU Triple block protein 1 OS=Poplar mosaic virus OX=12166 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.68 PLSPPPDD8 pKa = 3.35 HH9 pKa = 7.28 SKK11 pKa = 9.56 TFLVAAAGLSLVLCLYY27 pKa = 9.37 TLTRR31 pKa = 11.84 STLPGVGDD39 pKa = 4.47 NIHH42 pKa = 6.38 SLPHH46 pKa = 5.81 GGQYY50 pKa = 10.2 RR51 pKa = 11.84 DD52 pKa = 3.38 GTKK55 pKa = 10.35 SINYY59 pKa = 7.9 CSPGKK64 pKa = 10.32 SYY66 pKa = 10.77 PSSNLLRR73 pKa = 11.84 GGHH76 pKa = 6.09 FPALCAILLISGAILISYY94 pKa = 8.18 RR95 pKa = 11.84 FQPGALSRR103 pKa = 11.84 CGVTHH108 pKa = 7.36
Molecular weight: 11.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.856
IPC2_protein 8.595
IPC_protein 8.712
Toseland 8.697
ProMoST 8.873
Dawson 9.136
Bjellqvist 9.297
Wikipedia 9.224
Rodwell 9.165
Grimsley 8.712
Solomon 9.355
Lehninger 9.326
Nozaki 9.399
DTASelect 9.092
Thurlkill 9.107
EMBOSS 9.282
Sillero 9.385
Patrickios 2.371
IPC_peptide 9.341
IPC2_peptide 8.682
IPC2.peptide.svr19 8.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2856
71
1992
476.0
53.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.353 ± 1.13
3.046 ± 0.722
4.902 ± 0.416
7.143 ± 1.043
5.357 ± 0.508
6.513 ± 0.558
2.486 ± 0.463
5.112 ± 0.664
6.513 ± 0.902
9.174 ± 0.95
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.381 ± 0.404
3.887 ± 0.547
4.167 ± 0.612
2.556 ± 0.438
5.917 ± 0.83
7.983 ± 0.733
4.587 ± 0.657
6.443 ± 0.885
1.085 ± 0.199
3.396 ± 0.774
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here