Sanxia tombus-like virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGN8|A0A1L3KGN8_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 6 OX=1923390 PE=4 SV=1
MM1 pKa = 7.43SSAKK5 pKa = 9.95VVYY8 pKa = 10.01KK9 pKa = 10.4PSGHH13 pKa = 6.37CNEE16 pKa = 4.28SRR18 pKa = 11.84TAFTVIAALPSYY30 pKa = 10.11RR31 pKa = 11.84VAVHH35 pKa = 6.58KK36 pKa = 11.05NCICNEE42 pKa = 3.82LVSLHH47 pKa = 5.81NRR49 pKa = 11.84HH50 pKa = 6.35LRR52 pKa = 11.84QQAPGNQPYY61 pKa = 8.14TAAALSMWAPVYY73 pKa = 10.06DD74 pKa = 3.88VPRR77 pKa = 11.84ANIWDD82 pKa = 3.61IVKK85 pKa = 10.26GYY87 pKa = 10.78SGGKK91 pKa = 7.94RR92 pKa = 11.84KK93 pKa = 10.01AYY95 pKa = 10.22ARR97 pKa = 11.84AAINLQQPFQRR108 pKa = 11.84HH109 pKa = 4.09WAYY112 pKa = 10.72VSAFIKK118 pKa = 9.97PDD120 pKa = 4.11KK121 pKa = 10.89IPVDD125 pKa = 4.71EE126 pKa = 5.07IADD129 pKa = 3.73KK130 pKa = 10.97APRR133 pKa = 11.84LIQYY137 pKa = 9.77RR138 pKa = 11.84SKK140 pKa = 10.97EE141 pKa = 4.13YY142 pKa = 10.94NLLLATYY149 pKa = 8.51LKK151 pKa = 10.23PLEE154 pKa = 4.67DD155 pKa = 3.77AMFDD159 pKa = 3.54EE160 pKa = 5.02TDD162 pKa = 3.36PTGTLIFAKK171 pKa = 10.01KK172 pKa = 10.15RR173 pKa = 11.84NPQQRR178 pKa = 11.84AADD181 pKa = 4.36LLAKK185 pKa = 9.48MAAVEE190 pKa = 4.13DD191 pKa = 4.19ALVICADD198 pKa = 3.28HH199 pKa = 6.89SKK201 pKa = 10.6FDD203 pKa = 3.5SSVRR207 pKa = 11.84KK208 pKa = 8.04QHH210 pKa = 5.87LRR212 pKa = 11.84HH213 pKa = 6.21IEE215 pKa = 3.62AEE217 pKa = 4.11YY218 pKa = 10.18RR219 pKa = 11.84RR220 pKa = 11.84SYY222 pKa = 10.32PRR224 pKa = 11.84SKK226 pKa = 10.56LLKK229 pKa = 8.4RR230 pKa = 11.84LLRR233 pKa = 11.84LRR235 pKa = 11.84HH236 pKa = 5.02VNKK239 pKa = 10.06CRR241 pKa = 11.84TRR243 pKa = 11.84NGIKK247 pKa = 9.74YY248 pKa = 9.39QSKK251 pKa = 8.17EE252 pKa = 4.26TVQSGDD258 pKa = 3.17YY259 pKa = 9.43DD260 pKa = 3.54TGGRR264 pKa = 11.84NSRR267 pKa = 11.84LNAKK271 pKa = 9.61VLTSYY276 pKa = 10.9CILAGATTYY285 pKa = 11.42GLYY288 pKa = 9.72IDD290 pKa = 5.54GDD292 pKa = 4.13DD293 pKa = 4.2SVVIMPSALYY303 pKa = 10.18NEE305 pKa = 4.93KK306 pKa = 10.59AFKK309 pKa = 10.24DD310 pKa = 3.62HH311 pKa = 6.86CSKK314 pKa = 11.13LGFQTKK320 pKa = 9.26CQAYY324 pKa = 9.77RR325 pKa = 11.84DD326 pKa = 3.68LAEE329 pKa = 5.28ADD331 pKa = 3.83FCQAHH336 pKa = 7.56CIRR339 pKa = 11.84SDD341 pKa = 3.73PPTMMRR347 pKa = 11.84DD348 pKa = 3.71PLRR351 pKa = 11.84AAAHH355 pKa = 5.7FNISVRR361 pKa = 11.84NYY363 pKa = 10.42NGPAWPRR370 pKa = 11.84LVEE373 pKa = 4.2GKK375 pKa = 10.33LMCEE379 pKa = 3.78EE380 pKa = 4.06AAGRR384 pKa = 11.84GCPMMGKK391 pKa = 9.73LARR394 pKa = 11.84RR395 pKa = 11.84LRR397 pKa = 11.84TGIKK401 pKa = 10.04PIFDD405 pKa = 4.22PEE407 pKa = 4.41DD408 pKa = 3.46LEE410 pKa = 4.12KK411 pKa = 10.6WKK413 pKa = 10.57LVKK416 pKa = 10.36DD417 pKa = 4.25LPQATITMQARR428 pKa = 11.84LDD430 pKa = 3.95VYY432 pKa = 7.58TAWGFSVQDD441 pKa = 3.09QHH443 pKa = 7.71NFEE446 pKa = 4.45SMPRR450 pKa = 11.84HH451 pKa = 5.4CVVPEE456 pKa = 3.31IRR458 pKa = 11.84SRR460 pKa = 11.84RR461 pKa = 11.84NYY463 pKa = 10.04DD464 pKa = 3.11AEE466 pKa = 3.98SLHH469 pKa = 6.76RR470 pKa = 11.84VFEE473 pKa = 4.32AWSAMGATRR482 pKa = 11.84SEE484 pKa = 4.34SSWYY488 pKa = 10.39RR489 pKa = 11.84GTT491 pKa = 3.74

Molecular weight: 55.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGN8|A0A1L3KGN8_9VIRU Uncharacterized protein OS=Sanxia tombus-like virus 6 OX=1923390 PE=4 SV=1
MM1 pKa = 7.46TLNPYY6 pKa = 8.86TGYY9 pKa = 10.5SRR11 pKa = 11.84RR12 pKa = 11.84GVPWALLGARR22 pKa = 11.84AAGTVARR29 pKa = 11.84NAWQWYY35 pKa = 5.9NQPPAAPMYY44 pKa = 9.52GPPIPPPRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84QAPVYY60 pKa = 9.75APQPGPSQAAPRR72 pKa = 11.84GRR74 pKa = 11.84GRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84PRR81 pKa = 11.84GTGAPSRR88 pKa = 11.84PLATTKK94 pKa = 10.46EE95 pKa = 4.1GSNLVVRR102 pKa = 11.84DD103 pKa = 3.77TEE105 pKa = 4.57IISLGAKK112 pKa = 7.73NTLFTMQFKK121 pKa = 10.36PSEE124 pKa = 4.3TTLPRR129 pKa = 11.84LDD131 pKa = 3.91KK132 pKa = 11.27EE133 pKa = 4.23GGAFNRR139 pKa = 11.84YY140 pKa = 9.19RR141 pKa = 11.84IQYY144 pKa = 9.65VNIAYY149 pKa = 9.71ISTSSAATAGSVSYY163 pKa = 10.78GVLQGPTNANIATLNAIVKK182 pKa = 8.35LRR184 pKa = 11.84PMEE187 pKa = 4.25SHH189 pKa = 6.67AVWKK193 pKa = 10.7NSSLSLGSDD202 pKa = 2.66IMSAKK207 pKa = 10.06YY208 pKa = 10.51LSTAATGEE216 pKa = 4.11DD217 pKa = 3.06AVAFTVYY224 pKa = 10.2AWNTADD230 pKa = 4.14SPGYY234 pKa = 9.82LQFSYY239 pKa = 10.07RR240 pKa = 11.84VEE242 pKa = 4.01FAFPKK247 pKa = 10.03PP248 pKa = 3.48

Molecular weight: 26.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

739

248

491

369.5

41.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.502 ± 0.73

1.759 ± 0.917

4.465 ± 1.276

3.924 ± 0.574

2.842 ± 0.01

5.819 ± 1.17

2.165 ± 0.918

4.195 ± 0.295

5.413 ± 1.139

7.442 ± 0.516

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.219

4.195 ± 0.335

6.901 ± 1.657

3.789 ± 0.083

8.66 ± 0.11

7.037 ± 0.536

5.413 ± 1.172

5.413 ± 0.089

1.759 ± 0.134

4.871 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski