Bacteroides heparinolyticus
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2705 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P2A0K4|A0A3P2A0K4_9BACE ABC transporter ATP-binding protein OS=Bacteroides heparinolyticus OX=28113 GN=EII33_11270 PE=4 SV=1
MM1 pKa = 8.13 GMEE4 pKa = 5.94 DD5 pKa = 6.04 DD6 pKa = 5.66 FLQNDD11 pKa = 4.08 LDD13 pKa = 4.06 DD14 pKa = 4.74 EE15 pKa = 4.4 KK16 pKa = 10.66 TIEE19 pKa = 4.18 YY20 pKa = 9.37 IKK22 pKa = 10.96 NYY24 pKa = 10.0 LPQEE28 pKa = 4.18 LKK30 pKa = 11.13 GKK32 pKa = 10.47 FSDD35 pKa = 3.91 DD36 pKa = 2.9 EE37 pKa = 4.5 FYY39 pKa = 11.59 YY40 pKa = 10.87 FLDD43 pKa = 5.8 LIDD46 pKa = 4.53 EE47 pKa = 4.8 YY48 pKa = 11.34 YY49 pKa = 10.34 SEE51 pKa = 5.25 SGILDD56 pKa = 3.93 AQPDD60 pKa = 3.49 ADD62 pKa = 4.35 GYY64 pKa = 11.12 VEE66 pKa = 4.28 IDD68 pKa = 3.08 LGKK71 pKa = 10.28 VVDD74 pKa = 4.46 YY75 pKa = 10.52 IVKK78 pKa = 9.54 EE79 pKa = 3.85 AHH81 pKa = 6.39 KK82 pKa = 10.85 DD83 pKa = 3.49 EE84 pKa = 4.72 VGDD87 pKa = 3.92 YY88 pKa = 9.99 EE89 pKa = 4.21 PEE91 pKa = 4.37 EE92 pKa = 3.79 ILFIVQGEE100 pKa = 4.27 MEE102 pKa = 4.35 YY103 pKa = 10.38 TEE105 pKa = 4.49 SLGEE109 pKa = 3.98 EE110 pKa = 4.22
Molecular weight: 12.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.63
IPC_protein 3.617
Toseland 3.414
ProMoST 3.745
Dawson 3.592
Bjellqvist 3.795
Wikipedia 3.516
Rodwell 3.439
Grimsley 3.325
Solomon 3.579
Lehninger 3.541
Nozaki 3.719
DTASelect 3.897
Thurlkill 3.465
EMBOSS 3.528
Sillero 3.732
Patrickios 0.655
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|A0A3P2AAL5|A0A3P2AAL5_9BACE Peptidase S8 OS=Bacteroides heparinolyticus OX=28113 GN=EII33_03945 PE=4 SV=1
MM1 pKa = 7.53 WEE3 pKa = 3.82 INKK6 pKa = 10.6 GIGRR10 pKa = 11.84 TVEE13 pKa = 4.19 FKK15 pKa = 10.89 GLKK18 pKa = 9.01 SQYY21 pKa = 10.93 LFIFAGGLLATFILVVICYY40 pKa = 7.85 MCGMNQYY47 pKa = 9.83 LCLGMGVSGATLVVWQTFAMNRR69 pKa = 11.84 KK70 pKa = 8.42 YY71 pKa = 10.87 GEE73 pKa = 4.26 HH74 pKa = 6.75 GLMKK78 pKa = 10.22 RR79 pKa = 11.84 GAARR83 pKa = 11.84 RR84 pKa = 11.84 HH85 pKa = 4.98 PRR87 pKa = 11.84 YY88 pKa = 9.65 LLSRR92 pKa = 11.84 RR93 pKa = 11.84 PVRR96 pKa = 11.84 RR97 pKa = 11.84 ILSVRR102 pKa = 11.84 TVTTNEE108 pKa = 3.76 PTPP111 pKa = 3.75
Molecular weight: 12.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.94
IPC_protein 10.76
Toseland 10.628
ProMoST 10.409
Dawson 10.774
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.965
Grimsley 10.847
Solomon 10.877
Lehninger 10.833
Nozaki 10.643
DTASelect 10.526
Thurlkill 10.657
EMBOSS 11.038
Sillero 10.701
Patrickios 10.687
IPC_peptide 10.877
IPC2_peptide 9.736
IPC2.peptide.svr19 8.214
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2705
0
2705
1004298
28
2397
371.3
41.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.59 ± 0.047
1.261 ± 0.016
5.368 ± 0.031
6.627 ± 0.045
4.514 ± 0.028
7.044 ± 0.039
1.973 ± 0.019
6.486 ± 0.042
6.48 ± 0.036
9.202 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.022
4.862 ± 0.041
3.808 ± 0.027
3.311 ± 0.025
5.076 ± 0.034
5.96 ± 0.036
5.562 ± 0.031
6.515 ± 0.037
1.258 ± 0.016
4.319 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here