Streptococcus satellite phage Javan148

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFH5|A0A4D5ZFH5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan148 OX=2558542 GN=JavanS148_0007 PE=4 SV=1
MM1 pKa = 7.58AFISYY6 pKa = 10.18HH7 pKa = 5.33DD8 pKa = 3.69QQAEE12 pKa = 4.28HH13 pKa = 7.43LSMNYY18 pKa = 9.69QEE20 pKa = 5.02MNQDD24 pKa = 3.43DD25 pKa = 4.47LGAILEE31 pKa = 4.7TISQAFNHH39 pKa = 6.79LYY41 pKa = 11.22DD42 pKa = 3.83MVVEE46 pKa = 4.32GQLLVKK52 pKa = 10.69

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZEH7|A0A4D5ZEH7_9VIRU Aminoacyl-tRNA editing enzyme-like protein OS=Streptococcus satellite phage Javan148 OX=2558542 GN=JavanS148_0011 PE=3 SV=1
MM1 pKa = 7.31IMKK4 pKa = 8.92ITEE7 pKa = 4.11HH8 pKa = 6.3KK9 pKa = 9.71KK10 pKa = 9.75KK11 pKa = 10.59NGTIVYY17 pKa = 9.02RR18 pKa = 11.84ASIYY22 pKa = 10.8LGIDD26 pKa = 2.83QMTGKK31 pKa = 10.05RR32 pKa = 11.84VKK34 pKa = 9.97TSITGRR40 pKa = 11.84TRR42 pKa = 11.84KK43 pKa = 9.11EE44 pKa = 3.7VNQKK48 pKa = 10.29AKK50 pKa = 10.21HH51 pKa = 5.3AQFDD55 pKa = 4.08FLSNGSTIKK64 pKa = 10.43RR65 pKa = 11.84KK66 pKa = 9.88VVIKK70 pKa = 8.87TFKK73 pKa = 10.26EE74 pKa = 4.46LSHH77 pKa = 6.68LWLEE81 pKa = 4.6TYY83 pKa = 10.71KK84 pKa = 10.43LTVKK88 pKa = 10.0PQTYY92 pKa = 9.55DD93 pKa = 3.09ATVTRR98 pKa = 11.84LNRR101 pKa = 11.84HH102 pKa = 5.38IMPTLGNMKK111 pKa = 9.51VDD113 pKa = 4.87KK114 pKa = 9.89ITASDD119 pKa = 3.12IQMLINRR126 pKa = 11.84LSKK129 pKa = 11.11YY130 pKa = 8.49YY131 pKa = 11.04VNYY134 pKa = 8.12TAVRR138 pKa = 11.84SVIRR142 pKa = 11.84KK143 pKa = 8.39VLQQGVLLGLIDD155 pKa = 3.95YY156 pKa = 10.62NSARR160 pKa = 11.84DD161 pKa = 3.44IILPRR166 pKa = 11.84KK167 pKa = 8.91QPNAKK172 pKa = 9.88KK173 pKa = 9.75KK174 pKa = 10.82VKK176 pKa = 10.53FIDD179 pKa = 4.16PSDD182 pKa = 3.48LKK184 pKa = 11.33SFLEE188 pKa = 4.04HH189 pKa = 7.4LEE191 pKa = 4.21TSQHH195 pKa = 5.32KK196 pKa = 10.23RR197 pKa = 11.84YY198 pKa = 10.13NLYY201 pKa = 10.34FDD203 pKa = 4.37AVLYY207 pKa = 10.4QLLLSTGLRR216 pKa = 11.84IGEE219 pKa = 4.13ACALEE224 pKa = 4.31WGDD227 pKa = 3.94IDD229 pKa = 5.91LEE231 pKa = 4.47NGTIAINKK239 pKa = 7.38TYY241 pKa = 11.06NKK243 pKa = 9.77NLKK246 pKa = 9.76FLSTAKK252 pKa = 8.49TQSGNRR258 pKa = 11.84VISVDD263 pKa = 3.32KK264 pKa = 10.13KK265 pKa = 7.7TLRR268 pKa = 11.84SLKK271 pKa = 9.82LYY273 pKa = 9.48QMRR276 pKa = 11.84QRR278 pKa = 11.84QLFNEE283 pKa = 3.92VGARR287 pKa = 11.84VSEE290 pKa = 4.58VVFATPTRR298 pKa = 11.84KK299 pKa = 9.82YY300 pKa = 9.68FNASVRR306 pKa = 11.84QSALDD311 pKa = 3.79TRR313 pKa = 11.84CKK315 pKa = 9.91EE316 pKa = 3.83AGIEE320 pKa = 3.94RR321 pKa = 11.84FTFHH325 pKa = 7.65AFRR328 pKa = 11.84HH329 pKa = 4.69THH331 pKa = 6.92ASLLLNAGISYY342 pKa = 10.68KK343 pKa = 10.23EE344 pKa = 3.61LQYY347 pKa = 11.51RR348 pKa = 11.84LGHH351 pKa = 6.41ANISMTLDD359 pKa = 3.43TYY361 pKa = 11.88GHH363 pKa = 6.98LSKK366 pKa = 11.01DD367 pKa = 3.47KK368 pKa = 10.63EE369 pKa = 4.32KK370 pKa = 11.01EE371 pKa = 3.74AVLYY375 pKa = 9.78YY376 pKa = 10.38EE377 pKa = 4.79KK378 pKa = 11.4AMNNLL383 pKa = 3.41

Molecular weight: 44.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

4931

46

464

154.1

17.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.658 ± 0.279

0.71 ± 0.181

5.658 ± 0.341

7.483 ± 0.476

4.117 ± 0.249

4.644 ± 0.374

1.724 ± 0.189

7.747 ± 0.476

8.923 ± 0.394

9.998 ± 0.602

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.247

5.557 ± 0.348

3.022 ± 0.345

4.502 ± 0.28

4.745 ± 0.317

5.901 ± 0.428

5.841 ± 0.258

5.597 ± 0.266

1.014 ± 0.16

4.867 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski