Paracoccus aurantiacus
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3639 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C6S320|A0A5C6S320_9RHOB RNA methyltransferase OS=Paracoccus aurantiacus OX=2599412 GN=FQV27_11775 PE=3 SV=1
MM1 pKa = 8.34 DD2 pKa = 6.35 DD3 pKa = 3.97 NLQEE7 pKa = 4.39 GEE9 pKa = 4.11 PLIRR13 pKa = 11.84 PSTVEE18 pKa = 3.83 HH19 pKa = 6.82 PLYY22 pKa = 10.69 DD23 pKa = 3.69 QVVEE27 pKa = 4.32 ACRR30 pKa = 11.84 TVYY33 pKa = 10.74 DD34 pKa = 3.74 PEE36 pKa = 5.17 IPVNIYY42 pKa = 10.88 DD43 pKa = 4.22 LGLVYY48 pKa = 10.4 TIDD51 pKa = 3.59 IDD53 pKa = 4.0 NEE55 pKa = 4.11 ALVKK59 pKa = 10.9 VIMTLTAPGCPVAGEE74 pKa = 4.15 MPGWVADD81 pKa = 3.89 AVEE84 pKa = 4.47 SVPGVRR90 pKa = 11.84 EE91 pKa = 3.61 VDD93 pKa = 3.28 VEE95 pKa = 4.47 MTFQPQWGMDD105 pKa = 3.61 MMSEE109 pKa = 4.19 EE110 pKa = 4.48 ARR112 pKa = 11.84 LEE114 pKa = 4.05 LGFII118 pKa = 4.34
Molecular weight: 13.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.757
IPC_protein 3.694
Toseland 3.503
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.427
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.554
EMBOSS 3.605
Sillero 3.821
Patrickios 1.825
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.749
Protein with the highest isoelectric point:
>tr|A0A5C6S6F9|A0A5C6S6F9_9RHOB Polyphosphate kinase OS=Paracoccus aurantiacus OX=2599412 GN=ppk2 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 TRR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.63 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.43 AGRR29 pKa = 11.84 QVLNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.7 GRR40 pKa = 11.84 KK41 pKa = 8.85 RR42 pKa = 11.84 LSAA45 pKa = 3.96
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3639
0
3639
1145814
37
2543
314.9
34.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.764 ± 0.059
0.787 ± 0.012
6.292 ± 0.038
5.683 ± 0.034
3.568 ± 0.025
8.808 ± 0.046
1.984 ± 0.018
5.352 ± 0.029
2.901 ± 0.038
9.936 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.801 ± 0.021
2.573 ± 0.027
5.285 ± 0.034
3.229 ± 0.022
7.058 ± 0.038
5.216 ± 0.029
5.238 ± 0.029
6.944 ± 0.031
1.452 ± 0.016
2.128 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here