Lactobacillus phage Ld3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cequinquevirus; Lactobacillus virus Ld3

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075KJY6|A0A075KJY6_9CAUD Uncharacterized protein OS=Lactobacillus phage Ld3 OX=1500735 GN=LDB3_016 PE=4 SV=1
MM1 pKa = 7.32NSEE4 pKa = 4.22LEE6 pKa = 4.29KK7 pKa = 10.38IFKK10 pKa = 10.62NFTVNGKK17 pKa = 9.29QIPVSFLRR25 pKa = 11.84YY26 pKa = 9.39DD27 pKa = 3.77GKK29 pKa = 8.81EE30 pKa = 3.85TTYY33 pKa = 9.28ITYY36 pKa = 10.14QEE38 pKa = 4.02IMVDD42 pKa = 3.89EE43 pKa = 4.67VFSADD48 pKa = 4.2DD49 pKa = 4.49EE50 pKa = 4.53IVSYY54 pKa = 10.79SDD56 pKa = 5.23CYY58 pKa = 11.6DD59 pKa = 3.29FDD61 pKa = 4.35IYY63 pKa = 11.58SKK65 pKa = 11.33GNYY68 pKa = 8.75FPIVEE73 pKa = 4.19AVKK76 pKa = 10.61EE77 pKa = 3.88ILKK80 pKa = 11.04ANDD83 pKa = 3.09WVYY86 pKa = 11.41NPQNNSGDD94 pKa = 3.75LYY96 pKa = 10.99EE97 pKa = 6.17DD98 pKa = 3.35EE99 pKa = 4.52TGLYY103 pKa = 10.34HH104 pKa = 6.37KK105 pKa = 8.71TLNFIHH111 pKa = 6.77IRR113 pKa = 3.63

Molecular weight: 13.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075KQJ9|A0A075KQJ9_9CAUD Uncharacterized protein OS=Lactobacillus phage Ld3 OX=1500735 GN=LDB3_043 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.04PKK4 pKa = 10.63ALFADD9 pKa = 4.39SVSSSSSTISVGYY22 pKa = 8.8LAKK25 pKa = 10.21ILKK28 pKa = 10.05QNGINIGSIRR38 pKa = 11.84FFAWLRR44 pKa = 11.84DD45 pKa = 3.3NGYY48 pKa = 10.19LIKK51 pKa = 10.72RR52 pKa = 11.84QGSDD56 pKa = 3.54YY57 pKa = 11.17NSPTQYY63 pKa = 11.14SMEE66 pKa = 4.41RR67 pKa = 11.84GWFEE71 pKa = 4.21VKK73 pKa = 8.8EE74 pKa = 4.46TTVNYY79 pKa = 9.71PDD81 pKa = 4.0GRR83 pKa = 11.84TLVKK87 pKa = 9.55KK88 pKa = 8.57TPKK91 pKa = 9.27VTGKK95 pKa = 9.45GQQYY99 pKa = 9.02FVNRR103 pKa = 11.84FLKK106 pKa = 10.17MEE108 pKa = 4.38EE109 pKa = 4.27NEE111 pKa = 4.39DD112 pKa = 3.6NN113 pKa = 3.89

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

9132

33

968

186.4

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.559 ± 0.661

0.92 ± 0.18

6.581 ± 0.419

6.746 ± 0.48

3.811 ± 0.186

6.57 ± 0.379

1.314 ± 0.173

6.822 ± 0.282

8.782 ± 0.501

7.665 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.442 ± 0.177

6.724 ± 0.354

2.431 ± 0.171

3.318 ± 0.305

4.183 ± 0.306

6.57 ± 0.34

5.902 ± 0.334

7.03 ± 0.207

1.084 ± 0.115

4.523 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski