Amino acid dipepetide frequency for Quasibacillus thermotolerans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.259AlaAla: 8.259 ± 0.117	0.699AlaCys: 0.699 ± 0.025	3.973AlaAsp: 3.973 ± 0.066	5.989AlaGlu: 5.989 ± 0.089	3.779AlaPhe: 3.779 ± 0.065	6.462AlaGly: 6.462 ± 0.087	1.471AlaHis: 1.471 ± 0.036	5.698AlaIle: 5.698 ± 0.087	4.891AlaLys: 4.891 ± 0.076	7.996AlaLeu: 7.996 ± 0.082	2.301AlaMet: 2.301 ± 0.05	2.558AlaAsn: 2.558 ± 0.055	2.45AlaPro: 2.45 ± 0.054	2.413AlaGln: 2.413 ± 0.053	3.298AlaArg: 3.298 ± 0.058	4.74AlaSer: 4.74 ± 0.07	3.221AlaThr: 3.221 ± 0.057	6.678AlaVal: 6.678 ± 0.109	0.69AlaTrp: 0.69 ± 0.027	2.573AlaTyr: 2.573 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.489CysAla: 0.489 ± 0.023	0.099CysCys: 0.099 ± 0.012	0.405CysAsp: 0.405 ± 0.02	0.486CysGlu: 0.486 ± 0.022	0.323CysPhe: 0.323 ± 0.019	0.712CysGly: 0.712 ± 0.027	0.187CysHis: 0.187 ± 0.014	0.432CysIle: 0.432 ± 0.017	0.316CysLys: 0.316 ± 0.018	0.716CysLeu: 0.716 ± 0.026	0.172CysMet: 0.172 ± 0.013	0.225CysAsn: 0.225 ± 0.015	0.41CysPro: 0.41 ± 0.024	0.301CysGln: 0.301 ± 0.017	0.392CysArg: 0.392 ± 0.021	0.47CysSer: 0.47 ± 0.019	0.418CysThr: 0.418 ± 0.021	0.413CysVal: 0.413 ± 0.023	0.07CysTrp: 0.07 ± 0.007	0.261CysTyr: 0.261 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.123AspAla: 3.123 ± 0.062	0.353AspCys: 0.353 ± 0.02	2.101AspAsp: 2.101 ± 0.055	4.332AspGlu: 4.332 ± 0.072	2.128AspPhe: 2.128 ± 0.043	3.109AspGly: 3.109 ± 0.059	1.191AspHis: 1.191 ± 0.032	3.912AspIle: 3.912 ± 0.07	2.71AspLys: 2.71 ± 0.052	4.703AspLeu: 4.703 ± 0.068	1.364AspMet: 1.364 ± 0.038	1.511AspAsn: 1.511 ± 0.044	2.072AspPro: 2.072 ± 0.046	2.09AspGln: 2.09 ± 0.041	2.489AspArg: 2.489 ± 0.047	2.389AspSer: 2.389 ± 0.052	2.413AspThr: 2.413 ± 0.054	3.58AspVal: 3.58 ± 0.068	0.6AspTrp: 0.6 ± 0.026	2.002AspTyr: 2.002 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	7.06GluAla: 7.06 ± 0.093	0.384GluCys: 0.384 ± 0.018	3.772GluAsp: 3.772 ± 0.068	9.29GluGlu: 9.29 ± 0.118	2.482GluPhe: 2.482 ± 0.046	5.157GluGly: 5.157 ± 0.076	1.688GluHis: 1.688 ± 0.042	4.789GluIle: 4.789 ± 0.076	6.761GluLys: 6.761 ± 0.091	7.522GluLeu: 7.522 ± 0.093	2.385GluMet: 2.385 ± 0.05	3.119GluAsn: 3.119 ± 0.065	2.211GluPro: 2.211 ± 0.052	3.971GluGln: 3.971 ± 0.071	4.454GluArg: 4.454 ± 0.072	3.572GluSer: 3.572 ± 0.057	4.012GluThr: 4.012 ± 0.062	5.669GluVal: 5.669 ± 0.074	0.977GluTrp: 0.977 ± 0.032	2.113GluTyr: 2.113 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.163PheAla: 3.163 ± 0.06	0.347PheCys: 0.347 ± 0.018	2.082PheAsp: 2.082 ± 0.043	2.74PheGlu: 2.74 ± 0.045	2.392PhePhe: 2.392 ± 0.062	3.21PheGly: 3.21 ± 0.066	1.114PheHis: 1.114 ± 0.035	3.59PheIle: 3.59 ± 0.07	2.143PheLys: 2.143 ± 0.047	4.606PheLeu: 4.606 ± 0.081	1.191PheMet: 1.191 ± 0.035	1.564PheAsn: 1.564 ± 0.041	1.642PhePro: 1.642 ± 0.044	1.541PheGln: 1.541 ± 0.039	1.694PheArg: 1.694 ± 0.038	3.239PheSer: 3.239 ± 0.062	2.5PheThr: 2.5 ± 0.051	3.124PheVal: 3.124 ± 0.055	0.478PheTrp: 0.478 ± 0.023	1.664PheTyr: 1.664 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	5.537GlyAla: 5.537 ± 0.09	0.672GlyCys: 0.672 ± 0.028	3.086GlyAsp: 3.086 ± 0.06	5.11GlyGlu: 5.11 ± 0.08	3.355GlyPhe: 3.355 ± 0.053	5.06GlyGly: 5.06 ± 0.086	1.433GlyHis: 1.433 ± 0.036	5.326GlyIle: 5.326 ± 0.087	4.852GlyLys: 4.852 ± 0.076	6.738GlyLeu: 6.738 ± 0.089	2.225GlyMet: 2.225 ± 0.047	2.31GlyAsn: 2.31 ± 0.047	1.832GlyPro: 1.832 ± 0.051	2.425GlyGln: 2.425 ± 0.045	3.242GlyArg: 3.242 ± 0.055	3.99GlySer: 3.99 ± 0.061	4.184GlyThr: 4.184 ± 0.065	5.279GlyVal: 5.279 ± 0.065	0.916GlyTrp: 0.916 ± 0.03	2.732GlyTyr: 2.732 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.501HisAla: 1.501 ± 0.039	0.206HisCys: 0.206 ± 0.013	0.929HisAsp: 0.929 ± 0.029	1.519HisGlu: 1.519 ± 0.038	1.017HisPhe: 1.017 ± 0.033	1.475HisGly: 1.475 ± 0.034	0.667HisHis: 0.667 ± 0.033	1.439HisIle: 1.439 ± 0.035	1.015HisLys: 1.015 ± 0.03	2.288HisLeu: 2.288 ± 0.044	0.589HisMet: 0.589 ± 0.021	0.668HisAsn: 0.668 ± 0.029	1.294HisPro: 1.294 ± 0.041	0.85HisGln: 0.85 ± 0.029	1.033HisArg: 1.033 ± 0.033	1.341HisSer: 1.341 ± 0.037	1.195HisThr: 1.195 ± 0.037	1.468HisVal: 1.468 ± 0.036	0.237HisTrp: 0.237 ± 0.016	0.859HisTyr: 0.859 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.785IleAla: 5.785 ± 0.089	0.544IleCys: 0.544 ± 0.024	3.862IleAsp: 3.862 ± 0.064	5.578IleGlu: 5.578 ± 0.066	2.897IlePhe: 2.897 ± 0.054	5.64IleGly: 5.64 ± 0.084	1.754IleHis: 1.754 ± 0.04	4.937IleIle: 4.937 ± 0.081	3.733IleLys: 3.733 ± 0.069	6.173IleLeu: 6.173 ± 0.086	1.686IleMet: 1.686 ± 0.045	2.522IleAsn: 2.522 ± 0.05	3.09IlePro: 3.09 ± 0.053	2.883IleGln: 2.883 ± 0.056	3.15IleArg: 3.15 ± 0.058	4.301IleSer: 4.301 ± 0.063	3.782IleThr: 3.782 ± 0.065	5.068IleVal: 5.068 ± 0.08	0.589IleTrp: 0.589 ± 0.024	2.166IleTyr: 2.166 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	5.143LysAla: 5.143 ± 0.069	0.275LysCys: 0.275 ± 0.017	3.385LysAsp: 3.385 ± 0.067	7.567LysGlu: 7.567 ± 0.101	1.636LysPhe: 1.636 ± 0.039	4.493LysGly: 4.493 ± 0.064	1.236LysHis: 1.236 ± 0.039	3.529LysIle: 3.529 ± 0.067	5.542LysLys: 5.542 ± 0.097	5.29LysLeu: 5.29 ± 0.07	1.967LysMet: 1.967 ± 0.045	2.612LysAsn: 2.612 ± 0.053	2.186LysPro: 2.186 ± 0.049	3.127LysGln: 3.127 ± 0.058	3.583LysArg: 3.583 ± 0.058	3.041LysSer: 3.041 ± 0.058	3.15LysThr: 3.15 ± 0.06	4.499LysVal: 4.499 ± 0.065	0.8LysTrp: 0.8 ± 0.028	1.862LysTyr: 1.862 ± 0.047	0.0LysXaa: 0.0 ± 0.0
Leu	8.293LeuAla: 8.293 ± 0.103	0.596LeuCys: 0.596 ± 0.021	4.515LeuAsp: 4.515 ± 0.065	6.679LeuGlu: 6.679 ± 0.084	4.836LeuPhe: 4.836 ± 0.099	6.338LeuGly: 6.338 ± 0.074	2.088LeuHis: 2.088 ± 0.046	6.815LeuIle: 6.815 ± 0.1	6.307LeuLys: 6.307 ± 0.082	10.398LeuLeu: 10.398 ± 0.134	2.508LeuMet: 2.508 ± 0.051	3.747LeuAsn: 3.747 ± 0.059	4.241LeuPro: 4.241 ± 0.062	3.684LeuGln: 3.684 ± 0.071	4.042LeuArg: 4.042 ± 0.071	7.17LeuSer: 7.17 ± 0.089	5.59LeuThr: 5.59 ± 0.078	6.16LeuVal: 6.16 ± 0.079	0.798LeuTrp: 0.798 ± 0.031	3.103LeuTyr: 3.103 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.337MetAla: 2.337 ± 0.041	0.172MetCys: 0.172 ± 0.014	1.458MetAsp: 1.458 ± 0.036	2.249MetGlu: 2.249 ± 0.048	1.004MetPhe: 1.004 ± 0.036	1.713MetGly: 1.713 ± 0.044	0.467MetHis: 0.467 ± 0.026	2.068MetIle: 2.068 ± 0.042	2.502MetLys: 2.502 ± 0.045	2.531MetLeu: 2.531 ± 0.054	0.901MetMet: 0.901 ± 0.036	1.52MetAsn: 1.52 ± 0.036	1.106MetPro: 1.106 ± 0.033	0.936MetGln: 0.936 ± 0.029	1.214MetArg: 1.214 ± 0.036	1.726MetSer: 1.726 ± 0.043	1.711MetThr: 1.711 ± 0.04	1.706MetVal: 1.706 ± 0.039	0.195MetTrp: 0.195 ± 0.016	0.777MetTyr: 0.777 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.511AsnAla: 2.511 ± 0.051	0.309AsnCys: 0.309 ± 0.017	1.757AsnAsp: 1.757 ± 0.045	3.178AsnGlu: 3.178 ± 0.054	1.253AsnPhe: 1.253 ± 0.04	2.926AsnGly: 2.926 ± 0.056	0.893AsnHis: 0.893 ± 0.035	2.794AsnIle: 2.794 ± 0.05	2.329AsnLys: 2.329 ± 0.045	3.099AsnLeu: 3.099 ± 0.052	1.159AsnMet: 1.159 ± 0.032	1.456AsnAsn: 1.456 ± 0.04	1.803AsnPro: 1.803 ± 0.039	1.561AsnGln: 1.561 ± 0.045	1.918AsnArg: 1.918 ± 0.045	1.746AsnSer: 1.746 ± 0.038	1.811AsnThr: 1.811 ± 0.039	2.588AsnVal: 2.588 ± 0.043	0.477AsnTrp: 0.477 ± 0.022	1.202AsnTyr: 1.202 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	3.077ProAla: 3.077 ± 0.061	0.277ProCys: 0.277 ± 0.019	2.211ProAsp: 2.211 ± 0.05	3.31ProGlu: 3.31 ± 0.058	2.133ProPhe: 2.133 ± 0.046	2.624ProGly: 2.624 ± 0.051	0.883ProHis: 0.883 ± 0.03	2.548ProIle: 2.548 ± 0.051	1.962ProLys: 1.962 ± 0.043	3.877ProLeu: 3.877 ± 0.066	0.866ProMet: 0.866 ± 0.026	1.423ProAsn: 1.423 ± 0.037	1.279ProPro: 1.279 ± 0.04	1.154ProGln: 1.154 ± 0.033	1.25ProArg: 1.25 ± 0.036	2.47ProSer: 2.47 ± 0.047	1.673ProThr: 1.673 ± 0.039	3.242ProVal: 3.242 ± 0.059	0.382ProTrp: 0.382 ± 0.02	1.401ProTyr: 1.401 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.539GlnAla: 3.539 ± 0.063	0.209GlnCys: 0.209 ± 0.016	1.463GlnAsp: 1.463 ± 0.034	3.281GlnGlu: 3.281 ± 0.063	1.602GlnPhe: 1.602 ± 0.038	2.26GlnGly: 2.26 ± 0.047	0.775GlnHis: 0.775 ± 0.025	2.233GlnIle: 2.233 ± 0.049	2.591GlnLys: 2.591 ± 0.047	4.239GlnLeu: 4.239 ± 0.073	1.241GlnMet: 1.241 ± 0.033	1.281GlnAsn: 1.281 ± 0.037	1.548GlnPro: 1.548 ± 0.05	1.954GlnGln: 1.954 ± 0.054	1.776GlnArg: 1.776 ± 0.04	2.208GlnSer: 2.208 ± 0.049	2.103GlnThr: 2.103 ± 0.043	2.542GlnVal: 2.542 ± 0.05	0.429GlnTrp: 0.429 ± 0.019	1.234GlnTyr: 1.234 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	3.087ArgAla: 3.087 ± 0.061	0.288ArgCys: 0.288 ± 0.017	2.112ArgAsp: 2.112 ± 0.045	3.996ArgGlu: 3.996 ± 0.07	2.147ArgPhe: 2.147 ± 0.05	2.697ArgGly: 2.697 ± 0.058	0.938ArgHis: 0.938 ± 0.028	3.054ArgIle: 3.054 ± 0.057	3.586ArgLys: 3.586 ± 0.065	4.787ArgLeu: 4.787 ± 0.082	1.519ArgMet: 1.519 ± 0.04	1.7ArgAsn: 1.7 ± 0.039	1.619ArgPro: 1.619 ± 0.04	1.929ArgGln: 1.929 ± 0.042	2.398ArgArg: 2.398 ± 0.052	2.592ArgSer: 2.592 ± 0.054	2.362ArgThr: 2.362 ± 0.05	2.948ArgVal: 2.948 ± 0.057	0.467ArgTrp: 0.467 ± 0.021	1.728ArgTyr: 1.728 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	4.316SerAla: 4.316 ± 0.077	0.488SerCys: 0.488 ± 0.021	2.62SerAsp: 2.62 ± 0.047	3.998SerGlu: 3.998 ± 0.062	3.258SerPhe: 3.258 ± 0.059	4.459SerGly: 4.459 ± 0.062	1.293SerHis: 1.293 ± 0.037	4.453SerIle: 4.453 ± 0.061	3.348SerLys: 3.348 ± 0.06	6.329SerLeu: 6.329 ± 0.084	1.777SerMet: 1.777 ± 0.043	1.981SerAsn: 1.981 ± 0.041	2.344SerPro: 2.344 ± 0.049	1.927SerGln: 1.927 ± 0.044	2.718SerArg: 2.718 ± 0.05	3.911SerSer: 3.911 ± 0.069	2.828SerThr: 2.828 ± 0.044	4.21SerVal: 4.21 ± 0.052	0.685SerTrp: 0.685 ± 0.025	2.129SerTyr: 2.129 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	4.752ThrAla: 4.752 ± 0.083	0.35ThrCys: 0.35 ± 0.017	2.692ThrAsp: 2.692 ± 0.056	3.668ThrGlu: 3.668 ± 0.064	2.442ThrPhe: 2.442 ± 0.047	4.343ThrGly: 4.343 ± 0.073	1.014ThrHis: 1.014 ± 0.032	3.871ThrIle: 3.871 ± 0.058	3.03ThrLys: 3.03 ± 0.057	4.849ThrLeu: 4.849 ± 0.071	1.244ThrMet: 1.244 ± 0.039	1.996ThrAsn: 1.996 ± 0.041	2.31ThrPro: 2.31 ± 0.045	1.338ThrGln: 1.338 ± 0.035	1.97ThrArg: 1.97 ± 0.045	3.017ThrSer: 3.017 ± 0.056	2.555ThrThr: 2.555 ± 0.052	4.415ThrVal: 4.415 ± 0.062	0.471ThrTrp: 0.471 ± 0.018	1.774ThrTyr: 1.774 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	5.204ValAla: 5.204 ± 0.08	0.64ValCys: 0.64 ± 0.023	3.513ValAsp: 3.513 ± 0.064	5.093ValGlu: 5.093 ± 0.072	3.223ValPhe: 3.223 ± 0.07	4.447ValGly: 4.447 ± 0.078	1.523ValHis: 1.523 ± 0.032	5.679ValIle: 5.679 ± 0.081	4.596ValLys: 4.596 ± 0.075	7.169ValLeu: 7.169 ± 0.068	1.997ValMet: 1.997 ± 0.045	2.86ValAsn: 2.86 ± 0.05	2.908ValPro: 2.908 ± 0.048	2.653ValGln: 2.653 ± 0.052	3.09ValArg: 3.09 ± 0.057	4.626ValSer: 4.626 ± 0.056	4.196ValThr: 4.196 ± 0.069	5.077ValVal: 5.077 ± 0.078	0.692ValTrp: 0.692 ± 0.021	2.296ValTyr: 2.296 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.698TrpAla: 0.698 ± 0.028	0.08TrpCys: 0.08 ± 0.009	0.452TrpAsp: 0.452 ± 0.022	0.689TrpGlu: 0.689 ± 0.024	0.481TrpPhe: 0.481 ± 0.021	0.72TrpGly: 0.72 ± 0.026	0.22TrpHis: 0.22 ± 0.014	0.786TrpIle: 0.786 ± 0.028	0.734TrpLys: 0.734 ± 0.026	1.328TrpLeu: 1.328 ± 0.042	0.375TrpMet: 0.375 ± 0.018	0.455TrpAsn: 0.455 ± 0.023	0.328TrpPro: 0.328 ± 0.016	0.407TrpGln: 0.407 ± 0.019	0.446TrpArg: 0.446 ± 0.022	0.588TrpSer: 0.588 ± 0.023	0.584TrpThr: 0.584 ± 0.027	0.657TrpVal: 0.657 ± 0.027	0.129TrpTrp: 0.129 ± 0.012	0.29TrpTyr: 0.29 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.266TyrAla: 2.266 ± 0.046	0.307TyrCys: 0.307 ± 0.018	1.782TyrAsp: 1.782 ± 0.04	2.64TyrGlu: 2.64 ± 0.048	1.636TyrPhe: 1.636 ± 0.043	2.46TyrGly: 2.46 ± 0.043	0.758TyrHis: 0.758 ± 0.025	2.184TyrIle: 2.184 ± 0.042	1.977TyrLys: 1.977 ± 0.052	3.273TyrLeu: 3.273 ± 0.066	0.822TyrMet: 0.822 ± 0.026	1.264TyrAsn: 1.264 ± 0.038	1.371TyrPro: 1.371 ± 0.037	1.359TyrGln: 1.359 ± 0.034	1.797TyrArg: 1.797 ± 0.046	1.931TyrSer: 1.931 ± 0.045	1.811TyrThr: 1.811 ± 0.043	2.159TyrVal: 2.159 ± 0.046	0.367TyrTrp: 0.367 ± 0.019	1.29TyrTyr: 1.29 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4016 proteins (1082374 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---