Coccidioides immitis H538.4

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Onygenales incertae sedis; Coccidioides; Coccidioides immitis

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J8REK2|A0A0J8REK2_COCIT Uncharacterized protein OS=Coccidioides immitis H538.4 OX=396776 GN=CIHG_00793 PE=4 SV=1
MM1 pKa = 7.76ASIQTRR7 pKa = 11.84TDD9 pKa = 3.14VAPTGTSAVTSAVPTACGATTYY31 pKa = 10.2DD32 pKa = 4.32IPTKK36 pKa = 10.53DD37 pKa = 3.26AACAVPGTEE46 pKa = 5.17HH47 pKa = 7.14KK48 pKa = 10.98DD49 pKa = 3.73SMEE52 pKa = 4.27KK53 pKa = 10.76CCDD56 pKa = 3.65APVVTYY62 pKa = 11.16NEE64 pKa = 4.46GCGMYY69 pKa = 10.13CLASGGTVGDD79 pKa = 4.12LVKK82 pKa = 10.84CLIGDD87 pKa = 4.79GIADD91 pKa = 3.57GKK93 pKa = 9.37VFCNKK98 pKa = 8.87EE99 pKa = 3.83MNATATTTPTPTGRR113 pKa = 11.84DD114 pKa = 3.69DD115 pKa = 6.63DD116 pKa = 6.36DD117 pKa = 7.52DD118 pKa = 7.34DD119 pKa = 7.65DD120 pKa = 7.07DD121 pKa = 6.34DD122 pKa = 5.5EE123 pKa = 4.63PTNTDD128 pKa = 3.0GSPASTSSSAALANLPPQTLSKK150 pKa = 10.03PAIGVLFTLFFSMFAGVVFAA170 pKa = 6.02

Molecular weight: 17.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J8RID3|A0A0J8RID3_COCIT Sep4a OS=Coccidioides immitis H538.4 OX=396776 GN=CIHG_02196 PE=4 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.38QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10535

53

10588

3769138

39

4592

356.0

39.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.031 ± 0.022

1.341 ± 0.01

5.487 ± 0.018

6.244 ± 0.023

3.702 ± 0.016

6.653 ± 0.027

2.463 ± 0.011

5.062 ± 0.018

5.058 ± 0.019

8.974 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.009

3.734 ± 0.012

6.263 ± 0.031

3.988 ± 0.017

6.678 ± 0.023

8.644 ± 0.031

5.667 ± 0.015

5.838 ± 0.02

1.376 ± 0.01

2.633 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski