Agathobacter ruminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Agathobacter

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2568 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G3E424|A0A2G3E424_9FIRM Uncharacterized protein OS=Agathobacter ruminis OX=1712665 GN=CSX02_05540 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84KK3 pKa = 9.29RR4 pKa = 11.84LLALTMMSALTVTAFAGCGSDD25 pKa = 4.46KK26 pKa = 11.57SNTQKK31 pKa = 10.64PAGNAASAEE40 pKa = 4.1NPQAVSLKK48 pKa = 10.12LWTSEE53 pKa = 3.34EE54 pKa = 4.44DD55 pKa = 3.74YY56 pKa = 11.45EE57 pKa = 4.7LTEE60 pKa = 4.37DD61 pKa = 2.95MCARR65 pKa = 11.84FDD67 pKa = 3.87EE68 pKa = 4.35AHH70 pKa = 7.06PEE72 pKa = 4.13YY73 pKa = 10.89KK74 pKa = 9.96CDD76 pKa = 4.59FEE78 pKa = 4.09ITIINVDD85 pKa = 3.48EE86 pKa = 4.46SVEE89 pKa = 3.91QLEE92 pKa = 4.33TDD94 pKa = 3.68PDD96 pKa = 3.38NAADD100 pKa = 3.88VFIMPSGSIPQLVEE114 pKa = 3.64EE115 pKa = 4.6GLVYY119 pKa = 10.34PITYY123 pKa = 10.27DD124 pKa = 3.43ADD126 pKa = 4.03TLKK129 pKa = 11.17GLYY132 pKa = 9.96SEE134 pKa = 5.21GALDD138 pKa = 3.83ACSMNGEE145 pKa = 4.38LYY147 pKa = 10.61GVPFTPNSWFMFYY160 pKa = 10.88NKK162 pKa = 10.21KK163 pKa = 10.32LFNAEE168 pKa = 4.06EE169 pKa = 4.32VKK171 pKa = 10.99SLEE174 pKa = 3.88TMMAKK179 pKa = 10.46DD180 pKa = 5.27LGDD183 pKa = 3.47DD184 pKa = 4.0TYY186 pKa = 12.08NFACSLSNSWYY197 pKa = 9.62IEE199 pKa = 3.52AWFYY203 pKa = 11.6AAGCTLFGEE212 pKa = 5.28DD213 pKa = 3.72GTTPDD218 pKa = 3.89DD219 pKa = 4.15CTWNNANGLAAGEE232 pKa = 4.18YY233 pKa = 10.17IIDD236 pKa = 3.82LVNNPKK242 pKa = 10.66YY243 pKa = 10.51IEE245 pKa = 4.86DD246 pKa = 3.61KK247 pKa = 11.27DD248 pKa = 4.34GIAGSLMKK256 pKa = 10.24EE257 pKa = 4.29GKK259 pKa = 10.07LGAICTGTWGYY270 pKa = 10.33PEE272 pKa = 5.14LLEE275 pKa = 5.53ALGDD279 pKa = 3.96DD280 pKa = 4.64LGAVALPTVEE290 pKa = 4.52IAGKK294 pKa = 7.69TANLSNFADD303 pKa = 3.99YY304 pKa = 11.18KK305 pKa = 10.82CFAVKK310 pKa = 10.65SNTSAPLAAQQLAEE324 pKa = 4.28FFANEE329 pKa = 3.79EE330 pKa = 4.16NQLLRR335 pKa = 11.84YY336 pKa = 8.73QKK338 pKa = 11.03NSTPPTALSLTDD350 pKa = 3.61NADD353 pKa = 3.05IMSDD357 pKa = 2.79ISTCALLAQTEE368 pKa = 4.45FATPQPAISQINAYY382 pKa = 5.82WTPAEE387 pKa = 4.01ALGTGIVNGEE397 pKa = 3.62ITKK400 pKa = 10.93ANLQEE405 pKa = 4.43SLDD408 pKa = 3.92SLVQSVTTKK417 pKa = 10.8AFEE420 pKa = 3.83

Molecular weight: 45.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G3E5I3|A0A2G3E5I3_9FIRM DUF1292 domain-containing protein OS=Agathobacter ruminis OX=1712665 GN=CSX02_02060 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.82TVGGRR28 pKa = 11.84KK29 pKa = 8.92VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2568

0

2568

859823

29

2382

334.8

37.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.735 ± 0.052

1.462 ± 0.02

6.063 ± 0.041

7.49 ± 0.052

4.126 ± 0.031

6.685 ± 0.046

1.936 ± 0.02

7.438 ± 0.042

6.581 ± 0.046

8.658 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.152 ± 0.025

4.605 ± 0.037

3.187 ± 0.028

3.373 ± 0.028

4.329 ± 0.037

5.735 ± 0.036

5.378 ± 0.04

6.843 ± 0.04

0.892 ± 0.016

4.331 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski