bacterium HR30

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5ZMY5|A0A2H5ZMY5_9BACT Uncharacterized protein OS=bacterium HR30 OX=2035425 GN=HRbin30_02411 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.55GMLIGSLALSLLAWGCTKK20 pKa = 10.59GCEE23 pKa = 3.95RR24 pKa = 11.84KK25 pKa = 9.64SDD27 pKa = 3.63NAAPPAIAPQAEE39 pKa = 4.22QKK41 pKa = 10.81APAGAGEE48 pKa = 4.32AMTEE52 pKa = 4.01EE53 pKa = 4.6DD54 pKa = 4.59CVVLIDD60 pKa = 5.08ADD62 pKa = 4.06PDD64 pKa = 3.73YY65 pKa = 11.16GPPPLSVSFTSEE77 pKa = 4.16VEE79 pKa = 4.35CTGGEE84 pKa = 3.99AQYY87 pKa = 11.21KK88 pKa = 9.02WDD90 pKa = 4.46FGDD93 pKa = 4.23GSTSTEE99 pKa = 3.99PNPVHH104 pKa = 7.02TYY106 pKa = 9.44TKK108 pKa = 9.81VGEE111 pKa = 4.21YY112 pKa = 8.88TATLTVTAGEE122 pKa = 4.4VSASDD127 pKa = 4.02EE128 pKa = 3.74IDD130 pKa = 3.04ITVEE134 pKa = 3.86EE135 pKa = 4.7EE136 pKa = 4.3EE137 pKa = 4.14PAEE140 pKa = 4.12PQGSDD145 pKa = 2.63

Molecular weight: 15.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5ZKY8|A0A2H5ZKY8_9BACT Uncharacterized protein OS=bacterium HR30 OX=2035425 GN=HRbin30_01699 PE=4 SV=1
MM1 pKa = 7.24RR2 pKa = 11.84QPRR5 pKa = 11.84RR6 pKa = 11.84SLAWIRR12 pKa = 11.84LQEE15 pKa = 3.81KK16 pKa = 9.82SNRR19 pKa = 11.84FGAIRR24 pKa = 11.84LTVSPALRR32 pKa = 11.84AAVSMLAPALATANPRR48 pKa = 11.84EE49 pKa = 4.22VEE51 pKa = 4.12PVVQTIADD59 pKa = 4.53EE60 pKa = 4.26ICRR63 pKa = 11.84ALAVPRR69 pKa = 11.84VRR71 pKa = 11.84VSVEE75 pKa = 3.24LVRR78 pKa = 11.84PHH80 pKa = 5.41NHH82 pKa = 6.14FGEE85 pKa = 4.16LHH87 pKa = 5.8GLYY90 pKa = 9.56TAGPHH95 pKa = 5.62RR96 pKa = 11.84AATIRR101 pKa = 11.84LWMLTAKK108 pKa = 9.98RR109 pKa = 11.84KK110 pKa = 9.18QVAAFKK116 pKa = 10.14TFLRR120 pKa = 11.84TLLHH124 pKa = 6.08EE125 pKa = 4.8LCHH128 pKa = 6.72HH129 pKa = 7.18LDD131 pKa = 3.55YY132 pKa = 11.25TLLQLGGSVHH142 pKa = 7.14CEE144 pKa = 3.28GFYY147 pKa = 10.68KK148 pKa = 10.47RR149 pKa = 11.84EE150 pKa = 3.75SSLFHH155 pKa = 6.73QITAHH160 pKa = 6.66LRR162 pKa = 11.84ALEE165 pKa = 3.93QQ166 pKa = 3.51

Molecular weight: 18.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3301

0

3301

1077378

29

3100

326.4

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.032 ± 0.048

1.291 ± 0.024

4.651 ± 0.03

6.442 ± 0.052

3.849 ± 0.031

8.223 ± 0.042

2.197 ± 0.022

4.052 ± 0.034

2.574 ± 0.033

10.623 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.715 ± 0.019

2.414 ± 0.03

5.925 ± 0.041

3.564 ± 0.029

8.552 ± 0.049

5.191 ± 0.033

5.139 ± 0.047

8.589 ± 0.044

1.665 ± 0.022

2.312 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski