Torque teno mini virus 3
Average proteome isoelectric point is 7.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9JG51|Q9JG51_9VIRU Uncharacterized protein OS=Torque teno mini virus 3 OX=687371 PE=4 SV=1
MM1 pKa = 7.58 SFTFWKK7 pKa = 6.22 PTPYY11 pKa = 10.57 GHH13 pKa = 6.61 RR14 pKa = 11.84 QKK16 pKa = 11.07 EE17 pKa = 4.28 NNWINEE23 pKa = 4.08 IIHH26 pKa = 5.92 FHH28 pKa = 7.64 DD29 pKa = 4.15 NFCGCDD35 pKa = 3.48 EE36 pKa = 5.24 PIKK39 pKa = 10.69 HH40 pKa = 6.74 LIWKK44 pKa = 8.47 LAHH47 pKa = 6.61 NAPQLNIEE55 pKa = 4.5 KK56 pKa = 10.39 EE57 pKa = 4.13 DD58 pKa = 3.6 LQKK61 pKa = 10.87 LQKK64 pKa = 10.3 CLPTTTTDD72 pKa = 2.88 GTTGEE77 pKa = 4.49 DD78 pKa = 3.35 QHH80 pKa = 7.28 TDD82 pKa = 3.33 AADD85 pKa = 3.72 GDD87 pKa = 4.27 ADD89 pKa = 4.8 NIDD92 pKa = 4.0 EE93 pKa = 4.88 GDD95 pKa = 3.68 LDD97 pKa = 4.22 RR98 pKa = 11.84 LFEE101 pKa = 4.61 EE102 pKa = 5.7 DD103 pKa = 4.91 FGDD106 pKa = 3.85 DD107 pKa = 3.24 TRR109 pKa = 5.7
Molecular weight: 12.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.472
IPC2_protein 4.431
IPC_protein 4.38
Toseland 4.202
ProMoST 4.52
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.279
Rodwell 4.215
Grimsley 4.113
Solomon 4.355
Lehninger 4.317
Nozaki 4.469
DTASelect 4.698
Thurlkill 4.228
EMBOSS 4.291
Sillero 4.507
Patrickios 3.795
IPC_peptide 4.355
IPC2_peptide 4.495
IPC2.peptide.svr19 4.426
Protein with the highest isoelectric point:
>tr|Q9JG50|Q9JG50_9VIRU Capsid protein OS=Torque teno mini virus 3 OX=687371 PE=3 SV=1
MM1 pKa = 8.09 LFMTFFLSGEE11 pKa = 4.24 DD12 pKa = 4.95 AQHH15 pKa = 4.96 QWKK18 pKa = 10.19 KK19 pKa = 10.81 SKK21 pKa = 9.84 TPVTSHH27 pKa = 6.69 TFPSPLKK34 pKa = 10.03 SLKK37 pKa = 9.97 DD38 pKa = 3.49 VKK40 pKa = 11.04 SRR42 pKa = 11.84 IQTQTNQNTYY52 pKa = 8.5 TASTKK57 pKa = 10.45 DD58 pKa = 3.44 DD59 pKa = 3.69 KK60 pKa = 11.23 FLQKK64 pKa = 10.48 LLKK67 pKa = 9.97 KK68 pKa = 10.39 EE69 pKa = 4.02 YY70 pKa = 11.03 VKK72 pKa = 10.13 TLNLKK77 pKa = 9.37 HH78 pKa = 6.5 LCSQMDD84 pKa = 3.56 QNSTSQYY91 pKa = 9.0 SNKK94 pKa = 9.4 KK95 pKa = 8.53 KK96 pKa = 9.04 KK97 pKa = 9.53 HH98 pKa = 4.95 RR99 pKa = 11.84 RR100 pKa = 11.84 HH101 pKa = 6.28 RR102 pKa = 11.84 ARR104 pKa = 11.84 KK105 pKa = 7.7 KK106 pKa = 9.71 QKK108 pKa = 8.58 HH109 pKa = 5.61 HH110 pKa = 6.16 YY111 pKa = 8.77 SRR113 pKa = 11.84 SSSSSSDD120 pKa = 3.03 TRR122 pKa = 11.84 EE123 pKa = 3.93 SSSEE127 pKa = 4.1 SSSDD131 pKa = 3.14
Molecular weight: 15.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.108
IPC2_protein 9.502
IPC_protein 9.428
Toseland 10.482
ProMoST 9.955
Dawson 10.57
Bjellqvist 10.145
Wikipedia 10.672
Rodwell 11.286
Grimsley 10.599
Solomon 10.584
Lehninger 10.57
Nozaki 10.438
DTASelect 10.145
Thurlkill 10.452
EMBOSS 10.847
Sillero 10.467
Patrickios 11.008
IPC_peptide 10.599
IPC2_peptide 8.565
IPC2.peptide.svr19 8.542
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
901
109
661
300.3
35.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.553 ± 0.527
1.887 ± 0.381
5.216 ± 2.358
4.661 ± 0.925
4.44 ± 0.29
4.44 ± 1.222
3.441 ± 1.049
5.771 ± 1.749
9.101 ± 2.055
7.547 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.443 ± 0.283
6.992 ± 1.219
5.882 ± 1.476
6.215 ± 0.531
6.104 ± 1.057
6.437 ± 3.955
7.436 ± 0.922
2.442 ± 0.692
2.664 ± 0.678
5.327 ± 1.708
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here