Wenzhou tombus-like virus 1
Average proteome isoelectric point is 8.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KH37|A0A1L3KH37_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 1 OX=1923662 PE=3 SV=1
MM1 pKa = 7.12 VSAAFTFGCDD11 pKa = 2.81 VGTSILLLVAVVGVVLGVRR30 pKa = 11.84 WLRR33 pKa = 11.84 SSTTGATLVAEE44 pKa = 4.51 VDD46 pKa = 4.67 LGPDD50 pKa = 3.06 WTSVGVTTPYY60 pKa = 10.36 QRR62 pKa = 11.84 QLAYY66 pKa = 10.24 QFKK69 pKa = 11.11 AEE71 pKa = 4.13 FGEE74 pKa = 4.25 LRR76 pKa = 11.84 YY77 pKa = 10.71 NKK79 pKa = 10.0 ANRR82 pKa = 11.84 IIAGDD87 pKa = 3.6 WCRR90 pKa = 11.84 KK91 pKa = 9.38 AMADD95 pKa = 3.15 NDD97 pKa = 3.51 VRR99 pKa = 11.84 LTDD102 pKa = 3.73 RR103 pKa = 11.84 VRR105 pKa = 11.84 LLPLAVEE112 pKa = 4.7 LCLLPTRR119 pKa = 11.84 HH120 pKa = 5.83 AVAAAEE126 pKa = 3.89 LAATHH131 pKa = 5.78 EE132 pKa = 4.41 VRR134 pKa = 11.84 HH135 pKa = 5.7 RR136 pKa = 11.84 RR137 pKa = 11.84 AAADD141 pKa = 3.81 LPKK144 pKa = 9.71 XGCPAILPGVTTRR157 pKa = 11.84 VDD159 pKa = 3.51 RR160 pKa = 11.84 VGEE163 pKa = 3.76 ACLRR167 pKa = 11.84 VRR169 pKa = 11.84 EE170 pKa = 4.23 CVGVGVRR177 pKa = 11.84 SGVDD181 pKa = 2.86 RR182 pKa = 11.84 SVRR185 pKa = 11.84 YY186 pKa = 7.99 MAGFGTGVRR195 pKa = 11.84 YY196 pKa = 9.18 GVHH199 pKa = 6.34 CANLKK204 pKa = 9.66 NLARR208 pKa = 11.84 GIVEE212 pKa = 4.08 RR213 pKa = 11.84 VFHH216 pKa = 5.21 VVRR219 pKa = 11.84 DD220 pKa = 3.9 GRR222 pKa = 11.84 LAQAPQPRR230 pKa = 11.84 FGVFSRR236 pKa = 11.84 LNSVRR241 pKa = 11.84 QRR243 pKa = 11.84 LLAVTRR249 pKa = 11.84 PTPVVARR256 pKa = 11.84 EE257 pKa = 4.52 DD258 pKa = 3.89 YY259 pKa = 10.25 PGLYY263 pKa = 9.31 TGRR266 pKa = 11.84 KK267 pKa = 7.19 RR268 pKa = 11.84 GIYY271 pKa = 9.33 EE272 pKa = 3.9 RR273 pKa = 11.84 ALEE276 pKa = 4.21 SLTVRR281 pKa = 11.84 AINSRR286 pKa = 11.84 DD287 pKa = 2.83 AWVNTFVKK295 pKa = 10.53 AEE297 pKa = 4.02 KK298 pKa = 10.82 VNLDD302 pKa = 3.82 SKK304 pKa = 11.25 GDD306 pKa = 3.66 PAPRR310 pKa = 11.84 VIQPRR315 pKa = 11.84 SPRR318 pKa = 11.84 YY319 pKa = 7.87 NLEE322 pKa = 3.47 VGRR325 pKa = 11.84 YY326 pKa = 7.88 LKK328 pKa = 10.18 MFEE331 pKa = 5.17 RR332 pKa = 11.84 EE333 pKa = 3.93 LCHH336 pKa = 6.7 GFEE339 pKa = 4.6 RR340 pKa = 11.84 VWGYY344 pKa = 9.87 PVVLKK349 pKa = 10.79 GMNAQQVGGWMAAHH363 pKa = 7.16 WGAFRR368 pKa = 11.84 RR369 pKa = 11.84 PVAVGLDD376 pKa = 3.23 ASRR379 pKa = 11.84 FDD381 pKa = 3.33 QHH383 pKa = 7.36 VSYY386 pKa = 11.07 DD387 pKa = 3.46 ALRR390 pKa = 11.84 WEE392 pKa = 4.12 HH393 pKa = 6.33 SVYY396 pKa = 11.01 NSTFHH401 pKa = 7.78 SPEE404 pKa = 3.71 LAKK407 pKa = 10.7 LLRR410 pKa = 11.84 WQLHH414 pKa = 4.33 NRR416 pKa = 11.84 GVARR420 pKa = 11.84 TEE422 pKa = 4.41 GYY424 pKa = 10.43 RR425 pKa = 11.84 IDD427 pKa = 3.8 YY428 pKa = 9.84 DD429 pKa = 3.33 IRR431 pKa = 11.84 GCRR434 pKa = 11.84 MSGDD438 pKa = 3.53 INTGMGNCLLMSSMVIAYY456 pKa = 9.09 CEE458 pKa = 4.22 SVGIEE463 pKa = 3.61 YY464 pKa = 10.82 RR465 pKa = 11.84 LANNGDD471 pKa = 3.67 DD472 pKa = 3.37 CVLFVEE478 pKa = 4.57 QQDD481 pKa = 4.05 LVKK484 pKa = 10.93 LSGIDD489 pKa = 3.07 EE490 pKa = 4.19 WMLDD494 pKa = 3.49 FGFTLTRR501 pKa = 11.84 EE502 pKa = 3.91 EE503 pKa = 3.77 PAYY506 pKa = 10.54 VLEE509 pKa = 4.59 HH510 pKa = 7.73 VEE512 pKa = 4.54 FCQARR517 pKa = 11.84 PVFTSTGWRR526 pKa = 11.84 MVRR529 pKa = 11.84 DD530 pKa = 3.53 PRR532 pKa = 11.84 VAMSKK537 pKa = 10.67 DD538 pKa = 3.69 CVSLLGWEE546 pKa = 4.41 SDD548 pKa = 3.87 LDD550 pKa = 3.79 LQYY553 pKa = 10.15 WCHH556 pKa = 7.41 AIGTCGASLTAGVPVWHH573 pKa = 6.47 SWYY576 pKa = 10.3 HH577 pKa = 5.22 RR578 pKa = 11.84 LQRR581 pKa = 11.84 MGAVAPSGVVDD592 pKa = 3.57 MVYY595 pKa = 10.95 DD596 pKa = 3.56 SGLGYY601 pKa = 9.75 MSRR604 pKa = 11.84 GVVGGEE610 pKa = 3.8 VCAEE614 pKa = 3.71 ARR616 pKa = 11.84 VSFWRR621 pKa = 11.84 AFGITPDD628 pKa = 3.86 LQQSLEE634 pKa = 4.18 DD635 pKa = 4.1 EE636 pKa = 4.32 YY637 pKa = 11.88 SEE639 pKa = 4.2 AVVVAKK645 pKa = 9.03 PCPMTFPDD653 pKa = 4.07 VLAIDD658 pKa = 3.81 TSEE661 pKa = 4.33 NPLATWLVAARR672 pKa = 11.84 TPTLL676 pKa = 3.38
Molecular weight: 75.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.418
IPC2_protein 7.658
IPC_protein 7.439
Toseland 7.015
ProMoST 8.053
Dawson 8.141
Bjellqvist 8.639
Wikipedia 8.039
Rodwell 8.156
Grimsley 7.073
Solomon 8.17
Lehninger 8.2
Nozaki 8.843
DTASelect 8.287
Thurlkill 8.331
EMBOSS 8.346
Sillero 8.697
Patrickios 4.279
IPC_peptide 8.17
IPC2_peptide 7.98
IPC2.peptide.svr19 7.9
Protein with the highest isoelectric point:
>tr|A0A1L3KH37|A0A1L3KH37_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 1 OX=1923662 PE=3 SV=1
MM1 pKa = 7.32 ARR3 pKa = 11.84 SRR5 pKa = 11.84 KK6 pKa = 9.36 NPNPVTGGVRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 TRR21 pKa = 11.84 APNMKK26 pKa = 9.68 GGEE29 pKa = 3.93 AGTIVEE35 pKa = 4.32 YY36 pKa = 10.77 SAIGATVTTGGTGLANHH53 pKa = 6.45 KK54 pKa = 10.01 RR55 pKa = 11.84 VFIGGSPYY63 pKa = 11.26 DD64 pKa = 3.6 LTNTVGPSICSYY76 pKa = 11.28 YY77 pKa = 9.34 STCKK81 pKa = 10.54 FIPGTKK87 pKa = 9.22 IRR89 pKa = 11.84 WEE91 pKa = 3.99 PSVSFTTPGRR101 pKa = 11.84 VYY103 pKa = 11.05 VGFTDD108 pKa = 4.26 NPEE111 pKa = 3.98 TMTNIQSAATQADD124 pKa = 3.76 WNNLVKK130 pKa = 10.89 GLGDD134 pKa = 3.7 VISFPVWQEE143 pKa = 3.68 TEE145 pKa = 3.9 INFPTKK151 pKa = 10.3 LRR153 pKa = 11.84 RR154 pKa = 11.84 KK155 pKa = 9.3 MFDD158 pKa = 3.07 TNSAVSFVDD167 pKa = 3.97 VNALDD172 pKa = 4.23 RR173 pKa = 11.84 SAQIGMFVAVDD184 pKa = 4.11 GAPVTTSVGSFWYY197 pKa = 9.45 HH198 pKa = 6.75 DD199 pKa = 3.52 KK200 pKa = 11.44 VLVEE204 pKa = 5.07 GIQPTLTT211 pKa = 3.35
Molecular weight: 22.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.888
IPC2_protein 8.858
IPC_protein 8.887
Toseland 9.428
ProMoST 9.253
Dawson 9.721
Bjellqvist 9.472
Wikipedia 9.911
Rodwell 9.926
Grimsley 9.794
Solomon 9.794
Lehninger 9.75
Nozaki 9.545
DTASelect 9.428
Thurlkill 9.56
EMBOSS 9.853
Sillero 9.677
Patrickios 5.08
IPC_peptide 9.78
IPC2_peptide 8.2
IPC2.peptide.svr19 7.832
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
887
211
676
443.5
49.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.681 ± 0.976
2.255 ± 0.623
5.073 ± 0.385
4.735 ± 0.676
3.608 ± 0.54
8.794 ± 0.553
1.917 ± 0.462
3.157 ± 0.981
2.706 ± 0.744
7.892 ± 1.955
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.48 ± 0.053
3.382 ± 1.099
4.848 ± 0.626
2.593 ± 0.106
8.681 ± 1.202
5.75 ± 0.648
6.539 ± 2.306
11.161 ± 0.576
2.368 ± 0.225
3.269 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here