Tenacibaculum maritimum NCIMB 2154

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Tenacibaculum; Tenacibaculum maritimum

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H1E8D9|A0A2H1E8D9_9FLAO 4-hydroxy-tetrahydrodipicolinate reductase OS=Tenacibaculum maritimum NCIMB 2154 OX=1349785 GN=dapB PE=3 SV=1
MM1 pKa = 7.42IKK3 pKa = 10.13NYY5 pKa = 10.75SMSILKK11 pKa = 10.36LNSEE15 pKa = 4.55IKK17 pKa = 9.44STATFLLLLLLAGNFMYY34 pKa = 9.66GQCTDD39 pKa = 3.33GEE41 pKa = 5.13LNFTWSGQAGGGNQFVWNAGQRR63 pKa = 11.84TGSDD67 pKa = 3.12TFTSCASNVTVTVTVDD83 pKa = 3.29DD84 pKa = 5.05DD85 pKa = 4.59NNGSSEE91 pKa = 4.18VYY93 pKa = 10.64YY94 pKa = 10.85DD95 pKa = 4.25GSQSGGNSTSGGGAFANQGLTIWLDD120 pKa = 3.4NNEE123 pKa = 4.38NLFDD127 pKa = 4.76ADD129 pKa = 3.45AMVVGEE135 pKa = 4.25RR136 pKa = 11.84ATIVFDD142 pKa = 3.81FSVPVEE148 pKa = 4.14LKK150 pKa = 10.83SFLTGDD156 pKa = 3.62IDD158 pKa = 3.72WNNPGNISSTWRR170 pKa = 11.84DD171 pKa = 3.62FVRR174 pKa = 11.84VSASNGGTNVPVQGIVQAPGTVTMVGQLAASNLGAIGSTGGLDD217 pKa = 3.37PSDD220 pKa = 3.87PRR222 pKa = 11.84GHH224 pKa = 6.57VIWNSQGNLITSLRR238 pKa = 11.84VTYY241 pKa = 9.98EE242 pKa = 3.76VGVSGTGQQSLLIGSFLFDD261 pKa = 4.4ADD263 pKa = 3.76ADD265 pKa = 4.07NDD267 pKa = 4.15GVLNDD272 pKa = 3.52VDD274 pKa = 3.66QCEE277 pKa = 4.33GFDD280 pKa = 5.23DD281 pKa = 5.62NMDD284 pKa = 3.95ADD286 pKa = 4.49GDD288 pKa = 4.47GIADD292 pKa = 4.16GCDD295 pKa = 3.08LDD297 pKa = 5.36ADD299 pKa = 3.92NDD301 pKa = 4.71GILNVDD307 pKa = 4.4EE308 pKa = 4.73YY309 pKa = 11.18NNCITPTIEE318 pKa = 3.78LKK320 pKa = 10.66KK321 pKa = 10.99YY322 pKa = 10.17FLDD325 pKa = 4.7DD326 pKa = 4.49FGQGTVRR333 pKa = 11.84TSTPYY338 pKa = 10.11ISNSYY343 pKa = 9.63FFNSIADD350 pKa = 3.85VNDD353 pKa = 3.66GQYY356 pKa = 11.1CVIDD360 pKa = 3.95TPNPGAGNHH369 pKa = 5.45VSWGTYY375 pKa = 9.27GDD377 pKa = 3.71HH378 pKa = 7.47TIGDD382 pKa = 4.31TNGKK386 pKa = 8.25MLVINSSSSTFAHH399 pKa = 6.2YY400 pKa = 10.36YY401 pKa = 8.99KK402 pKa = 10.6RR403 pKa = 11.84SIEE406 pKa = 4.07NIIPNTIVKK415 pKa = 10.45LSFWARR421 pKa = 11.84NICAGCTSQPDD432 pKa = 3.33ITYY435 pKa = 10.5SILDD439 pKa = 3.35SSDD442 pKa = 3.43NVLATANTGGLSTSDD457 pKa = 2.72WVNYY461 pKa = 9.44EE462 pKa = 3.68LTVNIGSDD470 pKa = 3.18TDD472 pKa = 3.89LQISLTNNNVPSGDD486 pKa = 3.96GNDD489 pKa = 3.96LAIDD493 pKa = 4.96DD494 pKa = 4.77ISFSSIVCDD503 pKa = 3.78SDD505 pKa = 4.54SDD507 pKa = 4.3TIPNYY512 pKa = 10.88LDD514 pKa = 3.26VDD516 pKa = 4.33SDD518 pKa = 4.29NDD520 pKa = 3.92GCPDD524 pKa = 3.28AMEE527 pKa = 5.03GGNTGLNLTDD537 pKa = 5.5LNILNQLVGGVDD549 pKa = 3.89TNSSSASYY557 pKa = 10.01GVPIIAGGGQVDD569 pKa = 3.99VSSTNASVQSAEE581 pKa = 4.81CNPCDD586 pKa = 3.98ATSTLYY592 pKa = 10.75SDD594 pKa = 4.74RR595 pKa = 11.84DD596 pKa = 3.41GDD598 pKa = 4.19NVGDD602 pKa = 4.42ACDD605 pKa = 4.66SDD607 pKa = 4.55DD608 pKa = 5.62DD609 pKa = 4.53NDD611 pKa = 5.3GILDD615 pKa = 3.96EE616 pKa = 5.84NEE618 pKa = 4.17GCPSNFTPGEE628 pKa = 3.95VRR630 pKa = 11.84LIPPVGAWEE639 pKa = 4.02VFLYY643 pKa = 10.54DD644 pKa = 4.91GRR646 pKa = 11.84FQVLNALNLVDD657 pKa = 4.06VPSRR661 pKa = 11.84GADD664 pKa = 3.45GTPGGLVVLSAHH676 pKa = 6.57GFYY679 pKa = 10.13TGNVGTYY686 pKa = 7.61TFNDD690 pKa = 3.32VSIGANQDD698 pKa = 3.19PTPSVVASGVDD709 pKa = 3.48MKK711 pKa = 11.35VLTPFTVSNSGDD723 pKa = 2.72WSIVYY728 pKa = 10.03KK729 pKa = 9.91RR730 pKa = 11.84TIEE733 pKa = 4.02NNGTITIGAPGSYY746 pKa = 10.09VDD748 pKa = 4.87DD749 pKa = 4.65WIEE752 pKa = 3.77LFINGVLVDD761 pKa = 4.09SVNSFTASLPVADD774 pKa = 5.1VISEE778 pKa = 4.23NISAGDD784 pKa = 3.57VVEE787 pKa = 4.43VRR789 pKa = 11.84LTNGLGLGGFNLSIQTLNEE808 pKa = 3.39VTLAVVCDD816 pKa = 3.87TDD818 pKa = 3.38TDD820 pKa = 3.75NDD822 pKa = 3.9GVLNHH827 pKa = 7.35LDD829 pKa = 3.81TDD831 pKa = 4.28SDD833 pKa = 5.03DD834 pKa = 5.28DD835 pKa = 4.21GCPDD839 pKa = 4.48AIEE842 pKa = 4.39GAGNIIGGLSTLTGGSPTAGSSSQNLGTNVDD873 pKa = 3.48AEE875 pKa = 4.59GNPKK879 pKa = 9.6INAGDD884 pKa = 3.59TTGFEE889 pKa = 4.64QNNTAAVIDD898 pKa = 4.04NTDD901 pKa = 3.38STGCTADD908 pKa = 3.56LSLTKK913 pKa = 10.1KK914 pKa = 10.05VNKK917 pKa = 9.9ALPKK921 pKa = 10.07VGDD924 pKa = 4.52DD925 pKa = 2.76IVYY928 pKa = 10.27ILTVKK933 pKa = 10.97NEE935 pKa = 4.07GPLNASGVKK944 pKa = 8.82VTDD947 pKa = 3.55VLPVGLTYY955 pKa = 10.43KK956 pKa = 10.69SSVIDD961 pKa = 4.21LLGTYY966 pKa = 7.89TNNVWDD972 pKa = 3.92IGNLNVGEE980 pKa = 4.38SVNLRR985 pKa = 11.84ITATVTQQGTIINTAEE1001 pKa = 3.95ITQSNQVDD1009 pKa = 3.47IDD1011 pKa = 4.01STPMSGNN1018 pKa = 3.24

Molecular weight: 106.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H1EBK8|A0A2H1EBK8_9FLAO Trk system potassium uptake protein TrkA OS=Tenacibaculum maritimum NCIMB 2154 OX=1349785 GN=trkA PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 3.92TITTDD27 pKa = 3.73KK28 pKa = 10.69PEE30 pKa = 4.27KK31 pKa = 10.46SLLVPKK37 pKa = 10.16KK38 pKa = 10.51RR39 pKa = 11.84SGGRR43 pKa = 11.84NNQGRR48 pKa = 11.84MTTRR52 pKa = 11.84NIGGGHH58 pKa = 4.58KK59 pKa = 9.49QKK61 pKa = 10.8YY62 pKa = 9.58RR63 pKa = 11.84IIDD66 pKa = 3.85FKK68 pKa = 10.83RR69 pKa = 11.84DD70 pKa = 3.38KK71 pKa = 10.89QGIPATVKK79 pKa = 10.01TIEE82 pKa = 4.0YY83 pKa = 9.86DD84 pKa = 3.47PNRR87 pKa = 11.84TAFIALVSYY96 pKa = 11.47ADD98 pKa = 3.44GEE100 pKa = 4.21KK101 pKa = 10.25RR102 pKa = 11.84YY103 pKa = 10.74VIAQNGLKK111 pKa = 10.19VGQTIISGKK120 pKa = 9.71NVAPEE125 pKa = 4.07IGNAMYY131 pKa = 10.77LSDD134 pKa = 4.56IPLGTTISCIEE145 pKa = 4.05LRR147 pKa = 11.84PEE149 pKa = 3.6QGAVMARR156 pKa = 11.84SAGSFAQLMARR167 pKa = 11.84DD168 pKa = 3.96GKK170 pKa = 10.52YY171 pKa = 9.12ATVKK175 pKa = 10.42LPSGEE180 pKa = 4.06TRR182 pKa = 11.84LILLTCMATIGVVSNSDD199 pKa = 3.21HH200 pKa = 6.09QLLVSGKK207 pKa = 9.49AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TNAVRR224 pKa = 11.84MNPVDD229 pKa = 3.4HH230 pKa = 7.07PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.41GFKK256 pKa = 9.24TRR258 pKa = 11.84SKK260 pKa = 9.37TKK262 pKa = 10.4ASNKK266 pKa = 9.95YY267 pKa = 9.56IIEE270 pKa = 4.06RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.1KK274 pKa = 9.82

Molecular weight: 30.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2844

0

2844

983416

38

5927

345.8

39.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.107 ± 0.052

0.757 ± 0.017

5.063 ± 0.049

6.559 ± 0.048

5.191 ± 0.038

6.235 ± 0.05

1.847 ± 0.023

8.378 ± 0.04

8.989 ± 0.073

9.119 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.0 ± 0.024

6.39 ± 0.048

3.25 ± 0.023

3.182 ± 0.024

3.375 ± 0.034

6.394 ± 0.039

5.978 ± 0.073

5.938 ± 0.047

0.997 ± 0.015

4.252 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski