Methanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847) (Methanoplanus petrolearius)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanomicrobiaceae; Methanolacinia; Methanolacinia petrolearia

Average proteome isoelectric point is 5.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1RFT3|E1RFT3_METP4 ABC transporter related protein OS=Methanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847) OX=679926 GN=Mpet_0311 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.04KK3 pKa = 9.86HH4 pKa = 6.0VIAVLIAVAILSGIFFIAPCFFDD27 pKa = 3.89SSPGSEE33 pKa = 4.03DD34 pKa = 3.1ATLKK38 pKa = 10.68SVQNPDD44 pKa = 2.67TGSIHH49 pKa = 7.08RR50 pKa = 11.84EE51 pKa = 3.73NVSHH55 pKa = 7.27NEE57 pKa = 3.73TTEE60 pKa = 3.84PSGNDD65 pKa = 3.77CEE67 pKa = 4.93DD68 pKa = 3.58CCLVEE73 pKa = 5.51SGDD76 pKa = 3.37RR77 pKa = 11.84SYY79 pKa = 11.98GVMSIDD85 pKa = 4.98DD86 pKa = 3.97EE87 pKa = 4.58YY88 pKa = 11.64KK89 pKa = 10.03EE90 pKa = 4.25EE91 pKa = 4.48KK92 pKa = 10.63EE93 pKa = 3.9EE94 pKa = 4.18TINANPVVSYY104 pKa = 9.04ATEE107 pKa = 4.0NPSVGKK113 pKa = 8.88TDD115 pKa = 4.05FNDD118 pKa = 5.39DD119 pKa = 3.89IPDD122 pKa = 3.61SSGSGEE128 pKa = 4.12NLSGYY133 pKa = 9.62IPYY136 pKa = 10.52FGDD139 pKa = 3.39EE140 pKa = 4.44RR141 pKa = 11.84DD142 pKa = 3.4QGLCGNCWVWTCTEE156 pKa = 3.7IAEE159 pKa = 4.39IEE161 pKa = 4.52YY162 pKa = 10.68AVQTGNKK169 pKa = 8.55EE170 pKa = 4.15RR171 pKa = 11.84FSVQYY176 pKa = 10.71FNSNYY181 pKa = 10.22KK182 pKa = 10.53NGTGSGASASWACCGGNLFSFTGFYY207 pKa = 10.96DD208 pKa = 4.79LNDD211 pKa = 3.69QKK213 pKa = 11.75KK214 pKa = 10.35LIPWNNTKK222 pKa = 10.4ARR224 pKa = 11.84YY225 pKa = 9.13YY226 pKa = 11.0DD227 pKa = 4.33LLIKK231 pKa = 10.8CSDD234 pKa = 3.75GASNMQSALISEE246 pKa = 4.8DD247 pKa = 3.1PSVPFEE253 pKa = 4.14SVAPFTVQTWGISQSVAIDD272 pKa = 3.87TIEE275 pKa = 4.21SYY277 pKa = 10.74IDD279 pKa = 3.09QGRR282 pKa = 11.84ALYY285 pKa = 10.75VSFEE289 pKa = 4.33WVSGEE294 pKa = 5.96GFDD297 pKa = 4.09QWWSSEE303 pKa = 4.21SEE305 pKa = 3.99EE306 pKa = 4.94SVYY309 pKa = 11.25DD310 pKa = 4.17PIGDD314 pKa = 3.92MGNGTPDD321 pKa = 3.12SAHH324 pKa = 6.26AVTLIGYY331 pKa = 9.4NEE333 pKa = 4.34TAWLLHH339 pKa = 5.84NSWGTSSNHH348 pKa = 6.56PDD350 pKa = 2.83GTFWMTKK357 pKa = 10.4DD358 pKa = 3.22FDD360 pKa = 4.71YY361 pKa = 11.53NAGSLLFPSDD371 pKa = 3.66TYY373 pKa = 11.07VNFYY377 pKa = 10.78GFEE380 pKa = 3.97ITGLTEE386 pKa = 3.82TAPDD390 pKa = 4.78LIRR393 pKa = 11.84EE394 pKa = 4.1YY395 pKa = 10.65TYY397 pKa = 11.47GGTNADD403 pKa = 3.26AAYY406 pKa = 9.79GIVSASDD413 pKa = 3.06GGYY416 pKa = 10.18VLAGEE421 pKa = 4.54KK422 pKa = 10.16SSKK425 pKa = 9.76NGNYY429 pKa = 8.78PLNYY433 pKa = 9.46GSSDD437 pKa = 2.55TWMVKK442 pKa = 9.08TNSSGSVEE450 pKa = 3.78WEE452 pKa = 3.63INYY455 pKa = 10.1GGSGDD460 pKa = 3.78DD461 pKa = 3.18TAFAIIKK468 pKa = 9.57TSDD471 pKa = 3.44DD472 pKa = 3.46GFLIAGSTSSNDD484 pKa = 3.06INISGNNGGVDD495 pKa = 3.79ALLLKK500 pKa = 9.84TDD502 pKa = 4.01SNGVLQWLKK511 pKa = 11.02VYY513 pKa = 10.51GGSGDD518 pKa = 4.76DD519 pKa = 3.51SFNDD523 pKa = 3.2IEE525 pKa = 4.44AVSGGYY531 pKa = 9.84LAVGEE536 pKa = 4.53TKK538 pKa = 10.69SSGGDD543 pKa = 3.13VSSNNGGGDD552 pKa = 3.04AWIVKK557 pKa = 9.75VDD559 pKa = 3.53TSGDD563 pKa = 3.7ILWEE567 pKa = 3.86NTYY570 pKa = 10.78GGSADD575 pKa = 3.95EE576 pKa = 4.99SISDD580 pKa = 4.62IILPGDD586 pKa = 3.64GTLLASGSTASSDD599 pKa = 3.82GNVSSNNGGEE609 pKa = 4.15DD610 pKa = 2.7AWIIKK615 pKa = 9.14TGLSGDD621 pKa = 4.26LLWEE625 pKa = 3.9NTYY628 pKa = 10.86GGSGDD633 pKa = 3.78EE634 pKa = 4.0QALGIGVLSDD644 pKa = 3.06GSYY647 pKa = 10.34IVAGSAEE654 pKa = 4.23SSDD657 pKa = 3.77GDD659 pKa = 3.54VSSNNGCDD667 pKa = 3.81DD668 pKa = 3.03VWLFRR673 pKa = 11.84INSSGSLEE681 pKa = 3.91WEE683 pKa = 3.99KK684 pKa = 11.44NYY686 pKa = 10.95GGSGNDD692 pKa = 3.08AAASVLVLDD701 pKa = 4.92DD702 pKa = 6.33DD703 pKa = 5.11YY704 pKa = 11.92ILVCGATGSSDD715 pKa = 3.38GDD717 pKa = 3.62VAGNNGDD724 pKa = 3.6TDD726 pKa = 3.22VWLIKK731 pKa = 9.66TDD733 pKa = 3.43SSGDD737 pKa = 3.76IVWTGCHH744 pKa = 6.25GGQKK748 pKa = 9.94KK749 pKa = 10.4DD750 pKa = 4.02LIYY753 pKa = 10.77EE754 pKa = 4.35LVPSGSVNGFIGGGCTFSSDD774 pKa = 3.45GDD776 pKa = 3.59VSFNNGGGDD785 pKa = 3.3LWIAEE790 pKa = 4.04FGYY793 pKa = 10.0PDD795 pKa = 4.74EE796 pKa = 5.85NPMPDD801 pKa = 3.45PTVTPTQRR809 pKa = 11.84LTVTPTPTEE818 pKa = 4.09VYY820 pKa = 8.98TKK822 pKa = 10.83VSGEE826 pKa = 3.99PSSTSVDD833 pKa = 3.37LQAIIRR839 pKa = 11.84VMSEE843 pKa = 3.26KK844 pKa = 10.57DD845 pKa = 3.28YY846 pKa = 10.29TADD849 pKa = 3.1TGIEE853 pKa = 4.07GGEE856 pKa = 4.1QEE858 pKa = 5.33SDD860 pKa = 3.32GQQTEE865 pKa = 4.2TPPYY869 pKa = 10.07INAQKK874 pKa = 10.81VIGGTGDD881 pKa = 4.07DD882 pKa = 4.07FATSVIAGSSGFVVAGTVDD901 pKa = 3.38SDD903 pKa = 3.52EE904 pKa = 5.13GDD906 pKa = 3.33IEE908 pKa = 4.52SEE910 pKa = 4.0NGGADD915 pKa = 3.06ILLAGFDD922 pKa = 3.89SGLDD926 pKa = 3.79LVWQKK931 pKa = 11.44YY932 pKa = 9.85IGGSGDD938 pKa = 3.48DD939 pKa = 3.55TAVSIVKK946 pKa = 10.46SGDD949 pKa = 3.37DD950 pKa = 3.42SLVIAGTTSSSDD962 pKa = 2.84GDD964 pKa = 3.46IPLLHH969 pKa = 6.87GGSDD973 pKa = 3.15ILLACTDD980 pKa = 3.51GKK982 pKa = 11.62GDD984 pKa = 3.81IKK986 pKa = 10.19WVKK989 pKa = 9.95SAGGSLDD996 pKa = 3.91DD997 pKa = 4.75RR998 pKa = 11.84ASSMIMTDD1006 pKa = 3.21DD1007 pKa = 3.58GNFIVVGEE1015 pKa = 4.33TWSSDD1020 pKa = 2.74GDD1022 pKa = 3.24ISFEE1026 pKa = 3.75NRR1028 pKa = 11.84RR1029 pKa = 11.84NLGEE1033 pKa = 4.28GDD1035 pKa = 3.29LWAVCFDD1042 pKa = 3.67SSGNLVWEE1050 pKa = 4.3RR1051 pKa = 11.84RR1052 pKa = 11.84LGGTGYY1058 pKa = 10.98DD1059 pKa = 3.46SASAVAPAPGGGCTIAGQTRR1079 pKa = 11.84SYY1081 pKa = 11.48DD1082 pKa = 3.55GDD1084 pKa = 3.46PSGNNKK1090 pKa = 10.01HH1091 pKa = 6.25YY1092 pKa = 10.61GEE1094 pKa = 4.06SDD1096 pKa = 2.49IWVIRR1101 pKa = 11.84LDD1103 pKa = 3.45RR1104 pKa = 11.84YY1105 pKa = 10.8GRR1107 pKa = 11.84IIWQKK1112 pKa = 8.28TFGTSGEE1119 pKa = 4.49DD1120 pKa = 3.3GATDD1124 pKa = 3.59IEE1126 pKa = 4.5LSGDD1130 pKa = 3.04GCYY1133 pKa = 10.08IISGYY1138 pKa = 10.1AAAGDD1143 pKa = 3.89DD1144 pKa = 4.42DD1145 pKa = 5.0AAGSYY1150 pKa = 10.19GGSDD1154 pKa = 3.49ALILKK1159 pKa = 9.6IDD1161 pKa = 3.84SSGKK1165 pKa = 9.78LLWKK1169 pKa = 10.19RR1170 pKa = 11.84SYY1172 pKa = 11.2GSSSSDD1178 pKa = 2.97LGIAAEE1184 pKa = 4.44CCSNGDD1190 pKa = 3.51FVIAGVSFNKK1200 pKa = 9.02PGNMSSWGDD1209 pKa = 3.32YY1210 pKa = 10.77DD1211 pKa = 3.3GWLIRR1216 pKa = 11.84LDD1218 pKa = 3.61PSGTVLWEE1226 pKa = 3.8KK1227 pKa = 11.12DD1228 pKa = 3.5FGSSGEE1234 pKa = 4.02DD1235 pKa = 2.45WGYY1238 pKa = 11.17DD1239 pKa = 2.79IDD1241 pKa = 4.03YY1242 pKa = 10.32CAEE1245 pKa = 3.66EE1246 pKa = 4.81RR1247 pKa = 11.84EE1248 pKa = 4.24FIIAGMTTGLKK1259 pKa = 10.01DD1260 pKa = 3.46HH1261 pKa = 6.63QGSSDD1266 pKa = 3.63SDD1268 pKa = 3.87LLLVKK1273 pKa = 10.03IDD1275 pKa = 5.12DD1276 pKa = 4.51GSADD1280 pKa = 3.25

Molecular weight: 134.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1RIQ8|E1RIQ8_METP4 Putative nickel insertion protein OS=Methanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847) OX=679926 GN=Mpet_1898 PE=3 SV=1
MM1 pKa = 7.88APRR4 pKa = 11.84RR5 pKa = 11.84NKK7 pKa = 8.6NTEE10 pKa = 3.62RR11 pKa = 11.84KK12 pKa = 8.94IAEE15 pKa = 3.93EE16 pKa = 4.03RR17 pKa = 11.84IEE19 pKa = 4.12RR20 pKa = 11.84LFEE23 pKa = 4.12AAHH26 pKa = 5.34TVYY29 pKa = 10.61EE30 pKa = 4.57KK31 pKa = 11.05DD32 pKa = 3.21PSLSGRR38 pKa = 11.84YY39 pKa = 9.05VILAIKK45 pKa = 9.99IAMKK49 pKa = 10.54YY50 pKa = 9.04RR51 pKa = 11.84VKK53 pKa = 10.55IPDD56 pKa = 3.73RR57 pKa = 11.84YY58 pKa = 8.6RR59 pKa = 11.84HH60 pKa = 5.5SYY62 pKa = 9.16CRR64 pKa = 11.84KK65 pKa = 8.18CFSFFSPGNNTRR77 pKa = 11.84TRR79 pKa = 11.84INSGKK84 pKa = 8.64VTLTCTNCGYY94 pKa = 10.28IRR96 pKa = 11.84RR97 pKa = 11.84YY98 pKa = 8.57PIKK101 pKa = 10.15EE102 pKa = 3.69RR103 pKa = 11.84RR104 pKa = 3.5

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2779

0

2779

826422

30

2645

297.4

32.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.241 ± 0.048

1.349 ± 0.027

5.984 ± 0.039

7.475 ± 0.052

4.258 ± 0.034

7.901 ± 0.045

1.549 ± 0.02

8.424 ± 0.05

5.724 ± 0.048

8.67 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.024

4.081 ± 0.036

4.122 ± 0.028

2.148 ± 0.023

4.524 ± 0.042

6.95 ± 0.052

5.318 ± 0.047

7.007 ± 0.041

0.95 ± 0.019

3.609 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski