Mycobacterium phage Bobi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5VMV0|S5VMV0_9CAUD Glycosyltransferase OS=Mycobacterium phage Bobi OX=1340708 GN=107 PE=4 SV=1
MM1 pKa = 7.36AHH3 pKa = 6.42WKK5 pKa = 9.59YY6 pKa = 8.91WWTMPLLIAAGIIGPGLAAPEE27 pKa = 4.19AKK29 pKa = 10.36ADD31 pKa = 3.49ITSDD35 pKa = 3.54AFVMALDD42 pKa = 4.35SEE44 pKa = 5.11GITYY48 pKa = 10.39SSKK51 pKa = 10.3PAVINAGKK59 pKa = 10.01AVCDD63 pKa = 3.93VLDD66 pKa = 3.65TGYY69 pKa = 9.51TMYY72 pKa = 10.19EE73 pKa = 3.7ASVFVYY79 pKa = 10.8NNSNLDD85 pKa = 3.99LYY87 pKa = 11.33DD88 pKa = 3.2SGYY91 pKa = 10.79FVGAATASFCPEE103 pKa = 4.1HH104 pKa = 7.29LSGTGWVV111 pKa = 3.57

Molecular weight: 11.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5W017|S5W017_9CAUD Uncharacterized protein OS=Mycobacterium phage Bobi OX=1340708 GN=58 PE=4 SV=1
MM1 pKa = 6.74MVKK4 pKa = 9.83EE5 pKa = 3.83IWLPVPGYY13 pKa = 8.67EE14 pKa = 4.14GHH16 pKa = 6.37YY17 pKa = 9.82EE18 pKa = 3.96VSDD21 pKa = 3.79LGRR24 pKa = 11.84VRR26 pKa = 11.84SLTRR30 pKa = 11.84PVSNGRR36 pKa = 11.84GGLRR40 pKa = 11.84RR41 pKa = 11.84VTGRR45 pKa = 11.84VFARR49 pKa = 11.84KK50 pKa = 9.24LNRR53 pKa = 11.84YY54 pKa = 8.2GYY56 pKa = 9.84PCVCLRR62 pKa = 11.84KK63 pKa = 10.0NGLRR67 pKa = 11.84KK68 pKa = 10.28DD69 pKa = 3.9FTVHH73 pKa = 6.29CLVLAAFVGPRR84 pKa = 11.84PKK86 pKa = 11.07GMVARR91 pKa = 11.84HH92 pKa = 5.72LNADD96 pKa = 3.56RR97 pKa = 11.84SDD99 pKa = 3.37ARR101 pKa = 11.84LSNLCYY107 pKa = 9.59GTHH110 pKa = 6.4SEE112 pKa = 4.08NNYY115 pKa = 10.03DD116 pKa = 3.94RR117 pKa = 11.84VRR119 pKa = 11.84DD120 pKa = 3.72GHH122 pKa = 6.55DD123 pKa = 3.02WNSVKK128 pKa = 8.19THH130 pKa = 6.53CPRR133 pKa = 11.84GHH135 pKa = 7.16EE136 pKa = 4.01YY137 pKa = 9.6TADD140 pKa = 3.36NTYY143 pKa = 9.97TGPRR147 pKa = 11.84GNRR150 pKa = 11.84DD151 pKa = 2.73CRR153 pKa = 11.84ACIRR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84SRR161 pKa = 11.84EE162 pKa = 3.44YY163 pKa = 9.29SSRR166 pKa = 11.84RR167 pKa = 11.84KK168 pKa = 9.89SVALRR173 pKa = 11.84SGLSHH178 pKa = 6.25TAEE181 pKa = 4.21ASS183 pKa = 3.23

Molecular weight: 20.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

107

0

107

18806

29

1232

175.8

19.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.821 ± 0.394

1.218 ± 0.163

6.604 ± 0.21

5.785 ± 0.268

2.962 ± 0.192

8.885 ± 0.592

2.233 ± 0.173

4.312 ± 0.152

3.302 ± 0.171

7.397 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.118

3.323 ± 0.142

5.971 ± 0.215

3.499 ± 0.221

6.95 ± 0.382

5.95 ± 0.258

6.445 ± 0.253

7.21 ± 0.283

2.239 ± 0.155

2.616 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski