candidate division MSBL1 archaeon SCGC-AAA261F19

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133V920|A0A133V920_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA261F19 OX=1698275 GN=AKJ45_03045 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 5.39PLVWIGIAIGLVVGVPGAMSALAVGMAGVAAAGVTAEE39 pKa = 4.02EE40 pKa = 4.61PEE42 pKa = 4.01RR43 pKa = 11.84FGRR46 pKa = 11.84MFVLQILPGTQGFYY60 pKa = 11.28GFISGVLIMIGTGVLGGAVEE80 pKa = 4.5LEE82 pKa = 4.27PPIGMLALAAAVPAIIQGFTAYY104 pKa = 10.28GQGLVASASCGAVAKK119 pKa = 10.14EE120 pKa = 3.87PEE122 pKa = 4.37VFGKK126 pKa = 10.33SVVYY130 pKa = 9.23TVMPEE135 pKa = 4.12TYY137 pKa = 10.0AILGFLVTFLALIGLGVFF155 pKa = 4.26

Molecular weight: 15.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VBK3|A0A133VBK3_9EURY 50S ribosomal protein L24 OS=candidate division MSBL1 archaeon SCGC-AAA261F19 OX=1698275 GN=rpl24 PE=3 SV=1
MM1 pKa = 7.78TIGTRR6 pKa = 11.84LNCTDD11 pKa = 2.95NTGAKK16 pKa = 8.38EE17 pKa = 3.74LRR19 pKa = 11.84IISVIGYY26 pKa = 8.18KK27 pKa = 10.41GSRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84MSKK35 pKa = 10.67AGIGDD40 pKa = 3.62MAVVSVRR47 pKa = 11.84KK48 pKa = 8.02GTPEE52 pKa = 3.37MRR54 pKa = 11.84RR55 pKa = 11.84QVLRR59 pKa = 11.84AVIVRR64 pKa = 11.84QTKK67 pKa = 8.31EE68 pKa = 3.52YY69 pKa = 10.66RR70 pKa = 11.84RR71 pKa = 11.84VDD73 pKa = 3.02GMRR76 pKa = 11.84IKK78 pKa = 10.92FEE80 pKa = 4.84DD81 pKa = 3.46NAAVLMTPEE90 pKa = 4.8GNPQGSEE97 pKa = 3.3IRR99 pKa = 11.84GPIARR104 pKa = 11.84EE105 pKa = 3.78AAEE108 pKa = 4.03QWPLVAGIATMVVV121 pKa = 3.13

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

687

0

687

163887

35

1644

238.6

26.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.609 ± 0.111

1.115 ± 0.047

5.461 ± 0.073

9.251 ± 0.113

3.822 ± 0.068

7.631 ± 0.087

1.65 ± 0.038

7.015 ± 0.076

7.793 ± 0.119

9.466 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.043

3.443 ± 0.057

4.207 ± 0.068

2.303 ± 0.053

6.393 ± 0.087

6.238 ± 0.066

4.403 ± 0.06

7.096 ± 0.092

1.043 ± 0.038

2.781 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski