Cucumis melo (Muskmelon)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25056 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S3CIE2|A0A1S3CIE2_CUCME Isoform of A0A1S3CIS7 uncharacterized protein LOC103501308 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103501308 PE=4 SV=1
MM1 pKa = 7.45MKK3 pKa = 10.4QNLPVDD9 pKa = 4.38DD10 pKa = 5.01CVCDD14 pKa = 3.79YY15 pKa = 10.3YY16 pKa = 10.56TVKK19 pKa = 10.92NDD21 pKa = 3.34MEE23 pKa = 4.31IAEE26 pKa = 5.26DD27 pKa = 3.75DD28 pKa = 3.54ASYY31 pKa = 10.39PFPLKK36 pKa = 10.57QVDD39 pKa = 4.18EE40 pKa = 4.83LDD42 pKa = 3.33HH43 pKa = 7.08DD44 pKa = 4.27RR45 pKa = 11.84PSDD48 pKa = 3.59SDD50 pKa = 4.27YY51 pKa = 11.22EE52 pKa = 4.35TDD54 pKa = 5.47DD55 pKa = 4.53SNAEE59 pKa = 3.98NNPCFDD65 pKa = 3.91YY66 pKa = 10.96PDD68 pKa = 3.83EE69 pKa = 4.76EE70 pKa = 4.31EE71 pKa = 5.22LEE73 pKa = 4.4SEE75 pKa = 4.73SSNEE79 pKa = 3.94MMRR82 pKa = 11.84SSPLRR87 pKa = 11.84KK88 pKa = 9.52

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S3BY39|A0A1S3BY39_CUCME palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103494692 PE=3 SV=1
MM1 pKa = 7.19TVIINNINRR10 pKa = 11.84LSRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84SRR18 pKa = 11.84RR19 pKa = 11.84SRR21 pKa = 11.84SRR23 pKa = 11.84RR24 pKa = 11.84SSGRR28 pKa = 11.84SSSGRR33 pKa = 11.84SRR35 pKa = 11.84GGRR38 pKa = 11.84SRR40 pKa = 11.84RR41 pKa = 11.84GRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84GRR48 pKa = 11.84SRR50 pKa = 11.84SSGGRR55 pKa = 11.84SRR57 pKa = 11.84SSGGRR62 pKa = 11.84GSSSGGRR69 pKa = 11.84GSSSGGRR76 pKa = 11.84GSGSGGRR83 pKa = 11.84GSGSRR88 pKa = 11.84GSRR91 pKa = 11.84GSGSCGSVVVVVAVVVVAVGFSRR114 pKa = 11.84SSSSRR119 pKa = 11.84SRR121 pKa = 11.84SSRR124 pKa = 11.84SRR126 pKa = 11.84SSSSSSSSSRR136 pKa = 11.84SSSSSRR142 pKa = 11.84SRR144 pKa = 11.84SSRR147 pKa = 11.84SRR149 pKa = 11.84SSSSISGSSSSGSSSSGSSSSGSSSSGSSSSGSASSSGVAVVVEE193 pKa = 4.24

Molecular weight: 19.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19526

5530

25056

11448345

24

5432

456.9

51.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.485 ± 0.013

1.845 ± 0.007

5.318 ± 0.01

6.483 ± 0.017

4.352 ± 0.01

6.38 ± 0.014

2.418 ± 0.006

5.515 ± 0.011

6.115 ± 0.014

9.774 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.006

4.599 ± 0.009

4.837 ± 0.014

3.645 ± 0.009

5.292 ± 0.01

9.284 ± 0.018

4.831 ± 0.01

6.409 ± 0.01

1.268 ± 0.005

2.774 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski