DeBrazza s monkey arterivirus
Average proteome isoelectric point is 7.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B6CFJ6|A0A0B6CFJ6_9NIDO Nucleocapsid protein OS=DeBrazza's monkey arterivirus OX=1965063 GN=ORF7 PE=3 SV=1
MM1 pKa = 7.89 GDD3 pKa = 3.14 QLLRR7 pKa = 11.84 CVTPSCGIFLLLLCVLSCASNQTYY31 pKa = 10.09 ICLPSFNSSLLLTINASTHH50 pKa = 5.32 SCLFTGNVLVGSQYY64 pKa = 11.5 GLPICPQANEE74 pKa = 4.56 RR75 pKa = 11.84 YY76 pKa = 9.35 FDD78 pKa = 4.14 ANASGQLEE86 pKa = 4.98 LPTGLDD92 pKa = 3.69 LVFLRR97 pKa = 11.84 YY98 pKa = 8.87 VWQHH102 pKa = 6.1 LDD104 pKa = 3.3 QFPSLFNGSKK114 pKa = 10.38 PEE116 pKa = 3.94 QQASCWVINSTQPTSTPSLPQEE138 pKa = 3.9 YY139 pKa = 10.72 LEE141 pKa = 4.12 FWEE144 pKa = 4.7 FQPLPLLLLILVLQLVMLLPPLL166 pKa = 3.87
Molecular weight: 18.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.512
IPC2_protein 4.571
IPC_protein 4.38
Toseland 4.228
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.215
Rodwell 4.228
Grimsley 4.151
Solomon 4.317
Lehninger 4.279
Nozaki 4.457
DTASelect 4.584
Thurlkill 4.253
EMBOSS 4.228
Sillero 4.495
Patrickios 0.006
IPC_peptide 4.329
IPC2_peptide 4.482
IPC2.peptide.svr19 4.447
Protein with the highest isoelectric point:
>tr|A0A0B6CFI5|A0A0B6CFI5_9NIDO ORF2a' protein OS=DeBrazza's monkey arterivirus OX=1965063 GN=ORF2a' PE=4 SV=1
MM1 pKa = 7.87 PSQTGARR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 VDD12 pKa = 3.61 MVTTICSDD20 pKa = 3.29 PGYY23 pKa = 6.71 TTLAFTIAPVLLACLRR39 pKa = 11.84 LFRR42 pKa = 11.84 PSVRR46 pKa = 11.84 GVVCLLCIATLAYY59 pKa = 10.23 AATAFNEE66 pKa = 4.17 HH67 pKa = 6.34 SLATILTISFILVYY81 pKa = 10.65 LSYY84 pKa = 9.74 KK85 pKa = 7.7 TVAWVVIRR93 pKa = 11.84 CRR95 pKa = 11.84 MCRR98 pKa = 11.84 LGRR101 pKa = 11.84 QYY103 pKa = 9.16 ITAPSSMVEE112 pKa = 3.97 TSLGRR117 pKa = 11.84 SAITAGQSAVVRR129 pKa = 11.84 RR130 pKa = 11.84 TSGLTSANGLLVPDD144 pKa = 3.98 VKK146 pKa = 10.77 RR147 pKa = 11.84 IILHH151 pKa = 5.61 GRR153 pKa = 11.84 VAAKK157 pKa = 10.18 KK158 pKa = 10.45 GLVNLRR164 pKa = 11.84 KK165 pKa = 10.32 YY166 pKa = 10.37 GWQTKK171 pKa = 9.44 NKK173 pKa = 9.82
Molecular weight: 18.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.897
IPC_protein 10.804
Toseland 10.73
ProMoST 10.57
Dawson 10.847
Bjellqvist 10.628
Wikipedia 11.096
Rodwell 11.008
Grimsley 10.921
Solomon 10.979
Lehninger 10.935
Nozaki 10.76
DTASelect 10.613
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.789
Patrickios 10.716
IPC_peptide 10.979
IPC2_peptide 9.94
IPC2.peptide.svr19 8.306
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
1
13
7572
59
3587
582.5
63.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.509 ± 0.616
3.196 ± 0.204
4.226 ± 0.562
3.209 ± 0.203
4.437 ± 0.371
6.762 ± 0.719
2.773 ± 0.684
4.543 ± 0.375
3.711 ± 0.379
11.067 ± 0.6
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.373 ± 0.108
3.288 ± 0.362
6.194 ± 0.493
3.117 ± 0.367
5.137 ± 0.286
7.937 ± 0.578
6.894 ± 0.232
8.135 ± 0.625
1.149 ± 0.143
3.341 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here