Amino acid dipepetide frequency for Gracilibacillus orientalis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.446AlaAla: 4.446 ± 0.075	0.579AlaCys: 0.579 ± 0.02	3.484AlaAsp: 3.484 ± 0.059	4.512AlaGlu: 4.512 ± 0.078	3.139AlaPhe: 3.139 ± 0.054	4.605AlaGly: 4.605 ± 0.068	1.169AlaHis: 1.169 ± 0.029	5.851AlaIle: 5.851 ± 0.086	4.187AlaLys: 4.187 ± 0.063	6.316AlaLeu: 6.316 ± 0.076	1.818AlaMet: 1.818 ± 0.042	3.006AlaAsn: 3.006 ± 0.051	1.87AlaPro: 1.87 ± 0.043	2.055AlaGln: 2.055 ± 0.044	2.135AlaArg: 2.135 ± 0.039	3.852AlaSer: 3.852 ± 0.062	3.631AlaThr: 3.631 ± 0.056	4.631AlaVal: 4.631 ± 0.059	0.623AlaTrp: 0.623 ± 0.023	2.28AlaTyr: 2.28 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.333CysAla: 0.333 ± 0.015	0.099CysCys: 0.099 ± 0.01	0.373CysAsp: 0.373 ± 0.017	0.382CysGlu: 0.382 ± 0.016	0.302CysPhe: 0.302 ± 0.015	0.557CysGly: 0.557 ± 0.023	0.191CysHis: 0.191 ± 0.013	0.464CysIle: 0.464 ± 0.02	0.329CysLys: 0.329 ± 0.017	0.596CysLeu: 0.596 ± 0.024	0.172CysMet: 0.172 ± 0.013	0.294CysAsn: 0.294 ± 0.016	0.287CysPro: 0.287 ± 0.014	0.236CysGln: 0.236 ± 0.017	0.247CysArg: 0.247 ± 0.012	0.462CysSer: 0.462 ± 0.019	0.337CysThr: 0.337 ± 0.014	0.35CysVal: 0.35 ± 0.017	0.065CysTrp: 0.065 ± 0.007	0.255CysTyr: 0.255 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.269AspAla: 3.269 ± 0.05	0.317AspCys: 0.317 ± 0.019	3.149AspAsp: 3.149 ± 0.055	4.715AspGlu: 4.715 ± 0.061	2.627AspPhe: 2.627 ± 0.054	3.79AspGly: 3.79 ± 0.068	1.385AspHis: 1.385 ± 0.037	4.701AspIle: 4.701 ± 0.075	3.627AspLys: 3.627 ± 0.056	5.192AspLeu: 5.192 ± 0.065	1.451AspMet: 1.451 ± 0.036	2.68AspAsn: 2.68 ± 0.055	2.209AspPro: 2.209 ± 0.047	2.791AspGln: 2.791 ± 0.048	2.343AspArg: 2.343 ± 0.041	2.98AspSer: 2.98 ± 0.062	2.876AspThr: 2.876 ± 0.054	3.882AspVal: 3.882 ± 0.063	0.833AspTrp: 0.833 ± 0.028	2.551AspTyr: 2.551 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	5.167GluAla: 5.167 ± 0.075	0.326GluCys: 0.326 ± 0.017	4.61GluAsp: 4.61 ± 0.071	7.756GluGlu: 7.756 ± 0.118	2.508GluPhe: 2.508 ± 0.046	4.304GluGly: 4.304 ± 0.066	1.599GluHis: 1.599 ± 0.037	5.863GluIle: 5.863 ± 0.081	6.24GluLys: 6.24 ± 0.078	6.957GluLeu: 6.957 ± 0.075	2.302GluMet: 2.302 ± 0.04	4.333GluAsn: 4.333 ± 0.065	2.036GluPro: 2.036 ± 0.041	3.915GluGln: 3.915 ± 0.068	3.034GluArg: 3.034 ± 0.054	3.854GluSer: 3.854 ± 0.058	4.152GluThr: 4.152 ± 0.061	5.305GluVal: 5.305 ± 0.07	0.987GluTrp: 0.987 ± 0.031	2.456GluTyr: 2.456 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	2.885PheAla: 2.885 ± 0.045	0.314PheCys: 0.314 ± 0.017	2.682PheAsp: 2.682 ± 0.048	2.986PheGlu: 2.986 ± 0.046	2.37PhePhe: 2.37 ± 0.053	3.061PheGly: 3.061 ± 0.057	1.013PheHis: 1.013 ± 0.032	4.163PheIle: 4.163 ± 0.069	2.263PheLys: 2.263 ± 0.04	4.446PheLeu: 4.446 ± 0.078	1.169PheMet: 1.169 ± 0.036	2.081PheAsn: 2.081 ± 0.045	1.651PhePro: 1.651 ± 0.034	1.882PheGln: 1.882 ± 0.039	1.553PheArg: 1.553 ± 0.035	3.209PheSer: 3.209 ± 0.051	2.72PheThr: 2.72 ± 0.043	3.152PheVal: 3.152 ± 0.057	0.528PheTrp: 0.528 ± 0.024	1.836PheTyr: 1.836 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.28GlyAla: 4.28 ± 0.072	0.534GlyCys: 0.534 ± 0.022	3.416GlyAsp: 3.416 ± 0.066	4.685GlyGlu: 4.685 ± 0.074	3.256GlyPhe: 3.256 ± 0.059	4.311GlyGly: 4.311 ± 0.072	1.246GlyHis: 1.246 ± 0.034	5.542GlyIle: 5.542 ± 0.087	4.257GlyLys: 4.257 ± 0.061	6.02GlyLeu: 6.02 ± 0.078	1.993GlyMet: 1.993 ± 0.04	2.934GlyAsn: 2.934 ± 0.047	1.637GlyPro: 1.637 ± 0.044	2.124GlyGln: 2.124 ± 0.048	2.21GlyArg: 2.21 ± 0.043	3.917GlySer: 3.917 ± 0.061	3.679GlyThr: 3.679 ± 0.057	4.8GlyVal: 4.8 ± 0.066	0.792GlyTrp: 0.792 ± 0.027	2.844GlyTyr: 2.844 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.332HisAla: 1.332 ± 0.035	0.174HisCys: 0.174 ± 0.012	1.142HisAsp: 1.142 ± 0.032	1.399HisGlu: 1.399 ± 0.032	1.155HisPhe: 1.155 ± 0.034	1.322HisGly: 1.322 ± 0.037	0.78HisHis: 0.78 ± 0.029	1.617HisIle: 1.617 ± 0.037	0.97HisLys: 0.97 ± 0.03	2.147HisLeu: 2.147 ± 0.043	0.519HisMet: 0.519 ± 0.022	0.919HisAsn: 0.919 ± 0.032	1.08HisPro: 1.08 ± 0.028	1.067HisGln: 1.067 ± 0.032	0.822HisArg: 0.822 ± 0.028	1.201HisSer: 1.201 ± 0.029	1.106HisThr: 1.106 ± 0.031	1.429HisVal: 1.429 ± 0.04	0.259HisTrp: 0.259 ± 0.014	1.049HisTyr: 1.049 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.95IleAla: 5.95 ± 0.072	0.61IleCys: 0.61 ± 0.025	4.97IleAsp: 4.97 ± 0.063	6.007IleGlu: 6.007 ± 0.071	3.603IlePhe: 3.603 ± 0.062	5.945IleGly: 5.945 ± 0.077	1.744IleHis: 1.744 ± 0.038	6.523IleIle: 6.523 ± 0.103	4.61IleLys: 4.61 ± 0.066	7.283IleLeu: 7.283 ± 0.098	1.832IleMet: 1.832 ± 0.037	3.774IleAsn: 3.774 ± 0.058	3.487IlePro: 3.487 ± 0.057	3.071IleGln: 3.071 ± 0.051	2.94IleArg: 2.94 ± 0.05	5.253IleSer: 5.253 ± 0.08	4.794IleThr: 4.794 ± 0.062	5.421IleVal: 5.421 ± 0.076	0.755IleTrp: 0.755 ± 0.025	2.738IleTyr: 2.738 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.057LysAla: 4.057 ± 0.057	0.279LysCys: 0.279 ± 0.016	3.815LysAsp: 3.815 ± 0.055	6.428LysGlu: 6.428 ± 0.073	1.782LysPhe: 1.782 ± 0.036	3.899LysGly: 3.899 ± 0.066	1.372LysHis: 1.372 ± 0.037	4.409LysIle: 4.409 ± 0.061	5.443LysLys: 5.443 ± 0.076	5.264LysLeu: 5.264 ± 0.067	1.977LysMet: 1.977 ± 0.044	3.313LysAsn: 3.313 ± 0.053	2.018LysPro: 2.018 ± 0.037	3.51LysGln: 3.51 ± 0.064	2.898LysArg: 2.898 ± 0.052	3.384LysSer: 3.384 ± 0.061	3.288LysThr: 3.288 ± 0.06	4.467LysVal: 4.467 ± 0.059	0.889LysTrp: 0.889 ± 0.027	2.214LysTyr: 2.214 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	6.681LeuAla: 6.681 ± 0.077	0.576LeuCys: 0.576 ± 0.019	5.454LeuAsp: 5.454 ± 0.068	6.73LeuGlu: 6.73 ± 0.09	4.877LeuPhe: 4.877 ± 0.07	5.665LeuGly: 5.665 ± 0.08	1.951LeuHis: 1.951 ± 0.038	7.466LeuIle: 7.466 ± 0.103	5.849LeuLys: 5.849 ± 0.081	9.775LeuLeu: 9.775 ± 0.137	2.424LeuMet: 2.424 ± 0.05	4.243LeuAsn: 4.243 ± 0.058	3.762LeuPro: 3.762 ± 0.063	3.649LeuGln: 3.649 ± 0.063	3.173LeuArg: 3.173 ± 0.052	6.492LeuSer: 6.492 ± 0.074	5.596LeuThr: 5.596 ± 0.074	5.95LeuVal: 5.95 ± 0.071	0.866LeuTrp: 0.866 ± 0.025	3.208LeuTyr: 3.208 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	1.769MetAla: 1.769 ± 0.042	0.111MetCys: 0.111 ± 0.009	1.603MetAsp: 1.603 ± 0.03	2.216MetGlu: 2.216 ± 0.045	1.135MetPhe: 1.135 ± 0.03	1.55MetGly: 1.55 ± 0.043	0.469MetHis: 0.469 ± 0.02	2.267MetIle: 2.267 ± 0.048	2.287MetLys: 2.287 ± 0.041	2.518MetLeu: 2.518 ± 0.045	0.827MetMet: 0.827 ± 0.026	1.483MetAsn: 1.483 ± 0.035	0.951MetPro: 0.951 ± 0.029	0.991MetGln: 0.991 ± 0.029	0.973MetArg: 0.973 ± 0.031	1.615MetSer: 1.615 ± 0.039	1.683MetThr: 1.683 ± 0.037	1.82MetVal: 1.82 ± 0.041	0.213MetTrp: 0.213 ± 0.014	0.778MetTyr: 0.778 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.718AsnAla: 2.718 ± 0.047	0.277AsnCys: 0.277 ± 0.014	2.761AsnAsp: 2.761 ± 0.048	3.865AsnGlu: 3.865 ± 0.057	1.846AsnPhe: 1.846 ± 0.036	3.477AsnGly: 3.477 ± 0.054	1.204AsnHis: 1.204 ± 0.029	3.961AsnIle: 3.961 ± 0.063	3.217AsnLys: 3.217 ± 0.053	3.819AsnLeu: 3.819 ± 0.058	1.318AsnMet: 1.318 ± 0.034	2.574AsnAsn: 2.574 ± 0.058	2.077AsnPro: 2.077 ± 0.042	2.603AsnGln: 2.603 ± 0.046	1.995AsnArg: 1.995 ± 0.037	2.617AsnSer: 2.617 ± 0.05	2.488AsnThr: 2.488 ± 0.05	3.181AsnVal: 3.181 ± 0.042	0.693AsnTrp: 0.693 ± 0.025	1.926AsnTyr: 1.926 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	2.071ProAla: 2.071 ± 0.041	0.181ProCys: 0.181 ± 0.011	2.206ProAsp: 2.206 ± 0.042	3.073ProGlu: 3.073 ± 0.06	1.923ProPhe: 1.923 ± 0.044	2.122ProGly: 2.122 ± 0.05	0.76ProHis: 0.76 ± 0.023	2.917ProIle: 2.917 ± 0.054	1.988ProLys: 1.988 ± 0.043	3.277ProLeu: 3.277 ± 0.061	0.814ProMet: 0.814 ± 0.026	1.796ProAsn: 1.796 ± 0.034	0.861ProPro: 0.861 ± 0.028	1.051ProGln: 1.051 ± 0.031	0.95ProArg: 0.95 ± 0.028	2.219ProSer: 2.219 ± 0.041	2.065ProThr: 2.065 ± 0.042	2.681ProVal: 2.681 ± 0.047	0.41ProTrp: 0.41 ± 0.017	1.37ProTyr: 1.37 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.854GlnAla: 2.854 ± 0.052	0.191GlnCys: 0.191 ± 0.012	2.141GlnAsp: 2.141 ± 0.041	3.403GlnGlu: 3.403 ± 0.054	1.8GlnPhe: 1.8 ± 0.034	2.227GlnGly: 2.227 ± 0.043	0.929GlnHis: 0.929 ± 0.029	2.986GlnIle: 2.986 ± 0.05	2.737GlnLys: 2.737 ± 0.051	4.545GlnLeu: 4.545 ± 0.073	1.24GlnMet: 1.24 ± 0.037	1.911GlnAsn: 1.911 ± 0.042	1.358GlnPro: 1.358 ± 0.036	2.353GlnGln: 2.353 ± 0.054	1.464GlnArg: 1.464 ± 0.04	2.496GlnSer: 2.496 ± 0.045	2.28GlnThr: 2.28 ± 0.05	2.635GlnVal: 2.635 ± 0.048	0.502GlnTrp: 0.502 ± 0.018	1.564GlnTyr: 1.564 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.026ArgAla: 2.026 ± 0.038	0.221ArgCys: 0.221 ± 0.015	2.011ArgAsp: 2.011 ± 0.041	2.794ArgGlu: 2.794 ± 0.052	1.821ArgPhe: 1.821 ± 0.037	1.982ArgGly: 1.982 ± 0.043	0.773ArgHis: 0.773 ± 0.025	2.838ArgIle: 2.838 ± 0.046	2.914ArgLys: 2.914 ± 0.05	3.568ArgLeu: 3.568 ± 0.059	1.143ArgMet: 1.143 ± 0.029	1.848ArgAsn: 1.848 ± 0.042	1.106ArgPro: 1.106 ± 0.029	1.424ArgGln: 1.424 ± 0.035	1.46ArgArg: 1.46 ± 0.038	2.117ArgSer: 2.117 ± 0.039	1.866ArgThr: 1.866 ± 0.04	2.403ArgVal: 2.403 ± 0.046	0.449ArgTrp: 0.449 ± 0.02	1.511ArgTyr: 1.511 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	3.419SerAla: 3.419 ± 0.049	0.347SerCys: 0.347 ± 0.018	3.449SerAsp: 3.449 ± 0.061	4.356SerGlu: 4.356 ± 0.066	3.333SerPhe: 3.333 ± 0.052	4.173SerGly: 4.173 ± 0.059	1.215SerHis: 1.215 ± 0.033	5.315SerIle: 5.315 ± 0.065	3.637SerLys: 3.637 ± 0.055	6.007SerLeu: 6.007 ± 0.077	1.727SerMet: 1.727 ± 0.038	3.01SerAsn: 3.01 ± 0.048	1.92SerPro: 1.92 ± 0.045	2.087SerGln: 2.087 ± 0.038	1.998SerArg: 1.998 ± 0.038	3.931SerSer: 3.931 ± 0.07	3.262SerThr: 3.262 ± 0.055	4.079SerVal: 4.079 ± 0.052	0.714SerTrp: 0.714 ± 0.027	2.44SerTyr: 2.44 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	3.615ThrAla: 3.615 ± 0.059	0.344ThrCys: 0.344 ± 0.016	3.26ThrAsp: 3.26 ± 0.061	3.927ThrGlu: 3.927 ± 0.061	2.827ThrPhe: 2.827 ± 0.05	3.983ThrGly: 3.983 ± 0.055	1.064ThrHis: 1.064 ± 0.029	4.96ThrIle: 4.96 ± 0.064	3.287ThrLys: 3.287 ± 0.057	5.255ThrLeu: 5.255 ± 0.073	1.37ThrMet: 1.37 ± 0.038	2.931ThrAsn: 2.931 ± 0.047	2.181ThrPro: 2.181 ± 0.041	1.562ThrGln: 1.562 ± 0.038	1.647ThrArg: 1.647 ± 0.035	3.424ThrSer: 3.424 ± 0.059	3.239ThrThr: 3.239 ± 0.057	4.165ThrVal: 4.165 ± 0.068	0.64ThrTrp: 0.64 ± 0.021	2.159ThrTyr: 2.159 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	4.578ValAla: 4.578 ± 0.058	0.487ValCys: 0.487 ± 0.021	4.026ValAsp: 4.026 ± 0.06	5.218ValGlu: 5.218 ± 0.068	3.099ValPhe: 3.099 ± 0.055	4.323ValGly: 4.323 ± 0.072	1.329ValHis: 1.329 ± 0.035	5.749ValIle: 5.749 ± 0.073	4.112ValLys: 4.112 ± 0.06	6.413ValLeu: 6.413 ± 0.083	1.837ValMet: 1.837 ± 0.043	3.179ValAsn: 3.179 ± 0.052	2.409ValPro: 2.409 ± 0.044	2.356ValGln: 2.356 ± 0.048	2.34ValArg: 2.34 ± 0.045	4.599ValSer: 4.599 ± 0.063	4.197ValThr: 4.197 ± 0.075	4.781ValVal: 4.781 ± 0.069	0.699ValTrp: 0.699 ± 0.022	2.399ValTyr: 2.399 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.625TrpAla: 0.625 ± 0.022	0.072TrpCys: 0.072 ± 0.007	0.67TrpAsp: 0.67 ± 0.027	0.798TrpGlu: 0.798 ± 0.025	0.522TrpPhe: 0.522 ± 0.021	0.691TrpGly: 0.691 ± 0.026	0.257TrpHis: 0.257 ± 0.013	0.942TrpIle: 0.942 ± 0.03	0.803TrpLys: 0.803 ± 0.029	1.224TrpLeu: 1.224 ± 0.032	0.4TrpMet: 0.4 ± 0.018	0.659TrpAsn: 0.659 ± 0.02	0.271TrpPro: 0.271 ± 0.015	0.569TrpGln: 0.569 ± 0.019	0.443TrpArg: 0.443 ± 0.019	0.712TrpSer: 0.712 ± 0.026	0.625TrpThr: 0.625 ± 0.021	0.678TrpVal: 0.678 ± 0.023	0.171TrpTrp: 0.171 ± 0.014	0.431TrpTyr: 0.431 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.11TyrAla: 2.11 ± 0.041	0.292TyrCys: 0.292 ± 0.018	2.146TyrAsp: 2.146 ± 0.044	2.456TyrGlu: 2.456 ± 0.049	2.041TyrPhe: 2.041 ± 0.044	2.492TyrGly: 2.492 ± 0.043	1.039TyrHis: 1.039 ± 0.032	2.778TyrIle: 2.778 ± 0.048	1.95TyrLys: 1.95 ± 0.039	3.763TyrLeu: 3.763 ± 0.054	0.922TyrMet: 0.922 ± 0.027	1.649TyrAsn: 1.649 ± 0.044	1.564TyrPro: 1.564 ± 0.037	2.318TyrGln: 2.318 ± 0.055	1.648TyrArg: 1.648 ± 0.036	2.083TyrSer: 2.083 ± 0.038	1.958TyrThr: 1.958 ± 0.04	2.322TyrVal: 2.322 ± 0.05	0.476TyrTrp: 0.476 ± 0.02	1.637TyrTyr: 1.637 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4278 proteins (1301525 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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