Asystasia mosaic Madagascar virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5BIG2|A0A0C5BIG2_9GEMI Protein V2 OS=Asystasia mosaic Madagascar virus OX=1611435 GN=AV2 PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84PSSQSPTLSTLVPIKK17 pKa = 10.36VVHH20 pKa = 6.06RR21 pKa = 11.84QAKK24 pKa = 9.65KK25 pKa = 9.27KK26 pKa = 8.57RR27 pKa = 11.84QRR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 10.5KK32 pKa = 9.44IDD34 pKa = 3.66LGCGCSVLIHH44 pKa = 6.26PLCNNYY50 pKa = 9.5GFSHH54 pKa = 7.02RR55 pKa = 11.84GIHH58 pKa = 6.0HH59 pKa = 6.91CSSSAEE65 pKa = 4.0WNILLGTEE73 pKa = 4.38EE74 pKa = 4.81SNIHH78 pKa = 6.14NDD80 pKa = 3.09NRR82 pKa = 11.84THH84 pKa = 6.55RR85 pKa = 11.84QTIQHH90 pKa = 5.93EE91 pKa = 4.4PRR93 pKa = 11.84HH94 pKa = 5.46TEE96 pKa = 3.62HH97 pKa = 7.35PNQVQPQPEE106 pKa = 4.1EE107 pKa = 4.19GFRR110 pKa = 11.84DD111 pKa = 3.55SQMLFEE117 pKa = 4.66LQGLDD122 pKa = 3.71EE123 pKa = 5.12IEE125 pKa = 4.39EE126 pKa = 4.07FDD128 pKa = 3.7ISVLEE133 pKa = 4.38GFF135 pKa = 4.64

Molecular weight: 15.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5B4W5|A0A0C5B4W5_9GEMI Movement protein BC1 OS=Asystasia mosaic Madagascar virus OX=1611435 GN=BC1 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.89IIISTPASKK16 pKa = 10.49ARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.37SPRR27 pKa = 11.84VAASSVRR34 pKa = 11.84ITRR37 pKa = 11.84RR38 pKa = 11.84QAWVNRR44 pKa = 11.84PMYY47 pKa = 9.72RR48 pKa = 11.84KK49 pKa = 9.29PRR51 pKa = 11.84MYY53 pKa = 10.74RR54 pKa = 11.84MWRR57 pKa = 11.84SYY59 pKa = 10.78DD60 pKa = 3.44VPKK63 pKa = 10.87GCEE66 pKa = 4.43GPCKK70 pKa = 9.67VQSYY74 pKa = 8.13DD75 pKa = 3.16QRR77 pKa = 11.84DD78 pKa = 3.85DD79 pKa = 3.68VKK81 pKa = 10.43HH82 pKa = 6.0VGVVRR87 pKa = 11.84CISDD91 pKa = 3.47VTKK94 pKa = 11.06GPGLTHH100 pKa = 6.06RR101 pKa = 11.84TGKK104 pKa = 10.29RR105 pKa = 11.84FTVKK109 pKa = 9.99SVYY112 pKa = 9.87ICGKK116 pKa = 8.99IWMDD120 pKa = 4.13EE121 pKa = 4.13NIKK124 pKa = 10.06KK125 pKa = 9.97QNHH128 pKa = 4.76TNHH131 pKa = 5.66VLFFLTRR138 pKa = 11.84DD139 pKa = 3.28RR140 pKa = 11.84RR141 pKa = 11.84PYY143 pKa = 9.81GQAPMDD149 pKa = 4.27FGQVFNMFDD158 pKa = 3.99NEE160 pKa = 4.11PSTATVKK167 pKa = 10.85NDD169 pKa = 2.82LRR171 pKa = 11.84DD172 pKa = 3.34RR173 pKa = 11.84FQVLRR178 pKa = 11.84RR179 pKa = 11.84FYY181 pKa = 9.15ATVVGGPSGMKK192 pKa = 9.48EE193 pKa = 3.66QVLVRR198 pKa = 11.84RR199 pKa = 11.84FFKK202 pKa = 11.07LNAQIVYY209 pKa = 9.71NHH211 pKa = 6.32QDD213 pKa = 2.94GAKK216 pKa = 10.03YY217 pKa = 10.3EE218 pKa = 4.13NHH220 pKa = 6.44TEE222 pKa = 3.99NALLLYY228 pKa = 7.29MACTHH233 pKa = 7.07ASNPVYY239 pKa = 10.72ASLKK243 pKa = 8.47VRR245 pKa = 11.84IYY247 pKa = 10.71FYY249 pKa = 11.23DD250 pKa = 3.47SVSNN254 pKa = 3.9

Molecular weight: 29.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1658

65

362

207.3

23.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.187 ± 0.76

1.99 ± 0.314

4.463 ± 0.279

4.885 ± 0.822

4.704 ± 0.342

5.006 ± 0.284

3.257 ± 0.425

6.333 ± 0.826

5.669 ± 0.503

6.574 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.275

5.971 ± 0.55

5.127 ± 0.546

4.644 ± 0.496

7.419 ± 0.848

9.771 ± 1.319

5.428 ± 0.497

6.031 ± 0.918

1.086 ± 0.085

3.981 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski