Sewage-associated circular DNA virus-35

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI57|A0A0B4UI57_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-35 OX=1592102 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.59EE3 pKa = 4.04GRR5 pKa = 11.84EE6 pKa = 4.19SITTAKK12 pKa = 10.37HH13 pKa = 5.77FIGTIYY19 pKa = 10.42LPNASPQDD27 pKa = 3.5YY28 pKa = 9.95CAWNGRR34 pKa = 11.84TEE36 pKa = 4.14AAVHH40 pKa = 6.33LFEE43 pKa = 6.02ACVWQHH49 pKa = 6.52EE50 pKa = 4.48ICPDD54 pKa = 3.34TGRR57 pKa = 11.84HH58 pKa = 5.93HH59 pKa = 6.26IQIYY63 pKa = 8.03CHH65 pKa = 6.19ARR67 pKa = 11.84RR68 pKa = 11.84KK69 pKa = 9.51VRR71 pKa = 11.84YY72 pKa = 8.63AQLNDD77 pKa = 3.3ALGVVGSWKK86 pKa = 7.52TCKK89 pKa = 10.57GIAHH93 pKa = 7.83AIACYY98 pKa = 9.88AYY100 pKa = 8.94CTEE103 pKa = 4.35EE104 pKa = 4.61KK105 pKa = 10.44SDD107 pKa = 3.06ASSRR111 pKa = 11.84HH112 pKa = 4.81GCKK115 pKa = 10.08SSGWGDD121 pKa = 3.37PPGRR125 pKa = 11.84GAGGDD130 pKa = 3.47AAISVDD136 pKa = 3.82AAIEE140 pKa = 3.92FAKK143 pKa = 10.37SGKK146 pKa = 8.98RR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84EE150 pKa = 3.54LLMPEE155 pKa = 3.67NAEE158 pKa = 4.19ALRR161 pKa = 11.84GAYY164 pKa = 8.73LHH166 pKa = 6.51PAGFRR171 pKa = 11.84FVSEE175 pKa = 5.0LADD178 pKa = 3.58QPRR181 pKa = 11.84NTPDD185 pKa = 2.56EE186 pKa = 3.87WMQRR190 pKa = 11.84YY191 pKa = 7.75VICFWGGAGVGKK203 pKa = 9.52SRR205 pKa = 11.84RR206 pKa = 11.84VRR208 pKa = 11.84EE209 pKa = 3.78EE210 pKa = 3.49CAAAGLDD217 pKa = 3.68LWVAPASPGGLWFDD231 pKa = 3.89GYY233 pKa = 11.11DD234 pKa = 3.38RR235 pKa = 11.84HH236 pKa = 6.46PCALFDD242 pKa = 5.62DD243 pKa = 5.39FDD245 pKa = 7.09GDD247 pKa = 4.29MPWKK251 pKa = 10.13QLLCILEE258 pKa = 4.18GNIVQVQVKK267 pKa = 8.96GGHH270 pKa = 4.26VAFRR274 pKa = 11.84PRR276 pKa = 11.84VVYY279 pKa = 7.42FTADD283 pKa = 3.81CPPSSWTFSFGKK295 pKa = 10.71GEE297 pKa = 3.89ARR299 pKa = 11.84QGLNQARR306 pKa = 11.84LDD308 pKa = 3.47QLLRR312 pKa = 11.84RR313 pKa = 11.84FCHH316 pKa = 6.73IEE318 pKa = 4.0EE319 pKa = 4.19VSSARR324 pKa = 11.84DD325 pKa = 3.29YY326 pKa = 11.64RR327 pKa = 11.84EE328 pKa = 3.8ALNMNRR334 pKa = 11.84RR335 pKa = 11.84PAGPVPAGGEE345 pKa = 3.84GGANIEE351 pKa = 4.03IAPPLGEE358 pKa = 3.58AWEE361 pKa = 4.33NVHH364 pKa = 6.49VPQLPFDD371 pKa = 3.89VVPDD375 pKa = 3.7SEE377 pKa = 4.83YY378 pKa = 11.57VNEE381 pKa = 4.68LCNQQ385 pKa = 3.44

Molecular weight: 42.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI57|A0A0B4UI57_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-35 OX=1592102 PE=4 SV=1
MM1 pKa = 7.32SSKK4 pKa = 10.46RR5 pKa = 11.84KK6 pKa = 9.93RR7 pKa = 11.84NGQGEE12 pKa = 4.35GRR14 pKa = 11.84LVFSKK19 pKa = 10.42SRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 10.53GEE25 pKa = 3.63WDD27 pKa = 3.35SYY29 pKa = 10.81VPSSSHH35 pKa = 5.43SAMVAAKK42 pKa = 9.05ARR44 pKa = 11.84QRR46 pKa = 11.84RR47 pKa = 11.84GYY49 pKa = 10.68GSVARR54 pKa = 11.84TRR56 pKa = 11.84GAAVQGEE63 pKa = 4.64TKK65 pKa = 10.92YY66 pKa = 11.01FDD68 pKa = 3.89TEE70 pKa = 4.15LQPTAISAVTTTWVAGTLVDD90 pKa = 3.59PTTTINLGDD99 pKa = 3.84AAIATPGCLFAPKK112 pKa = 10.1VSAALNGRR120 pKa = 11.84IGRR123 pKa = 11.84KK124 pKa = 8.75VLIKK128 pKa = 10.19KK129 pKa = 9.86VKK131 pKa = 7.98LTGVINVPTHH141 pKa = 5.83SAQATGDD148 pKa = 3.58AGTKK152 pKa = 8.92IRR154 pKa = 11.84VMLVLDD160 pKa = 3.62QQTNASAMTSAQLLNDD176 pKa = 4.13AGNALTTISSFQNPNNFGRR195 pKa = 11.84FKK197 pKa = 10.78VLKK200 pKa = 10.31DD201 pKa = 3.03KK202 pKa = 10.93TFFLGDD208 pKa = 3.45LAMTGAATAIEE219 pKa = 4.12QSGTVIPFKK228 pKa = 10.09MSYY231 pKa = 8.87VFKK234 pKa = 10.76RR235 pKa = 11.84GLRR238 pKa = 11.84VDD240 pKa = 5.26FNATNGGTVADD251 pKa = 4.78IIDD254 pKa = 3.82NSLHH258 pKa = 5.22VVCGANNVQLIPSLSYY274 pKa = 9.5YY275 pKa = 10.83SRR277 pKa = 11.84VSYY280 pKa = 11.31VDD282 pKa = 3.12

Molecular weight: 30.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

667

282

385

333.5

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.094 ± 0.067

2.549 ± 1.222

5.097 ± 0.324

4.798 ± 2.009

3.898 ± 0.002

9.445 ± 0.385

2.399 ± 0.887

4.348 ± 0.174

4.348 ± 1.116

6.597 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.649 ± 0.318

4.198 ± 0.745

4.948 ± 1.166

3.748 ± 0.101

6.897 ± 0.577

6.447 ± 1.371

5.397 ± 2.539

7.346 ± 1.009

1.949 ± 0.824

2.849 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski