Amino acid dipepetide frequency for Bacillus phage BSP10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.187AlaAla: 5.187 ± 0.614
0.355AlaCys: 0.355 ± 0.101
4.034AlaAsp: 4.034 ± 0.313
4.566AlaGlu: 4.566 ± 0.376
2.217AlaPhe: 2.217 ± 0.238
3.679AlaGly: 3.679 ± 0.449
1.064AlaHis: 1.064 ± 0.155
3.99AlaIle: 3.99 ± 0.289
4.145AlaLys: 4.145 ± 0.376
5.94AlaLeu: 5.94 ± 0.317
1.64AlaMet: 1.64 ± 0.172
2.571AlaAsn: 2.571 ± 0.26
2.305AlaPro: 2.305 ± 0.233
2.349AlaGln: 2.349 ± 0.278
2.372AlaArg: 2.372 ± 0.205
3.192AlaSer: 3.192 ± 0.36
3.724AlaThr: 3.724 ± 0.392
3.923AlaVal: 3.923 ± 0.326
0.709AlaTrp: 0.709 ± 0.129
2.793AlaTyr: 2.793 ± 0.243
0.0AlaXaa: 0.0 ± 0.0
Cys
0.443CysAla: 0.443 ± 0.099
0.133CysCys: 0.133 ± 0.069
0.488CysAsp: 0.488 ± 0.095
0.421CysGlu: 0.421 ± 0.114
0.377CysPhe: 0.377 ± 0.096
0.598CysGly: 0.598 ± 0.139
0.111CysHis: 0.111 ± 0.045
0.532CysIle: 0.532 ± 0.109
0.665CysLys: 0.665 ± 0.099
0.731CysLeu: 0.731 ± 0.133
0.222CysMet: 0.222 ± 0.072
0.399CysAsn: 0.399 ± 0.098
0.355CysPro: 0.355 ± 0.092
0.155CysGln: 0.155 ± 0.061
0.332CysArg: 0.332 ± 0.085
0.709CysSer: 0.709 ± 0.122
0.31CysThr: 0.31 ± 0.084
0.621CysVal: 0.621 ± 0.128
0.111CysTrp: 0.111 ± 0.042
0.421CysTyr: 0.421 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
3.613AspAla: 3.613 ± 0.319
0.421AspCys: 0.421 ± 0.114
3.391AspAsp: 3.391 ± 0.383
4.788AspGlu: 4.788 ± 0.391
3.103AspPhe: 3.103 ± 0.337
4.189AspGly: 4.189 ± 0.43
1.086AspHis: 1.086 ± 0.161
5.164AspIle: 5.164 ± 0.359
4.61AspLys: 4.61 ± 0.355
5.763AspLeu: 5.763 ± 0.451
2.327AspMet: 2.327 ± 0.262
3.037AspAsn: 3.037 ± 0.259
2.061AspPro: 2.061 ± 0.199
2.172AspGln: 2.172 ± 0.188
2.815AspArg: 2.815 ± 0.27
3.702AspSer: 3.702 ± 0.331
4.167AspThr: 4.167 ± 0.302
5.031AspVal: 5.031 ± 0.402
0.776AspTrp: 0.776 ± 0.136
3.413AspTyr: 3.413 ± 0.287
0.0AspXaa: 0.0 ± 0.0
Glu
5.342GluAla: 5.342 ± 0.355
0.554GluCys: 0.554 ± 0.12
5.94GluAsp: 5.94 ± 0.425
9.553GluGlu: 9.553 ± 0.681
2.837GluPhe: 2.837 ± 0.262
5.563GluGly: 5.563 ± 0.346
1.662GluHis: 1.662 ± 0.195
5.009GluIle: 5.009 ± 0.386
5.874GluLys: 5.874 ± 0.401
7.226GluLeu: 7.226 ± 0.407
2.682GluMet: 2.682 ± 0.246
3.702GluAsn: 3.702 ± 0.275
2.084GluPro: 2.084 ± 0.289
2.992GluGln: 2.992 ± 0.289
3.835GluArg: 3.835 ± 0.383
3.768GluSer: 3.768 ± 0.287
3.569GluThr: 3.569 ± 0.29
6.228GluVal: 6.228 ± 0.421
1.042GluTrp: 1.042 ± 0.156
3.546GluTyr: 3.546 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
1.729PheAla: 1.729 ± 0.2
0.576PheCys: 0.576 ± 0.112
2.571PheAsp: 2.571 ± 0.247
2.926PheGlu: 2.926 ± 0.288
1.419PhePhe: 1.419 ± 0.214
2.283PheGly: 2.283 ± 0.237
0.754PheHis: 0.754 ± 0.125
2.837PheIle: 2.837 ± 0.283
2.859PheLys: 2.859 ± 0.248
3.103PheLeu: 3.103 ± 0.278
0.887PheMet: 0.887 ± 0.132
2.327PheAsn: 2.327 ± 0.219
1.33PhePro: 1.33 ± 0.158
1.219PheGln: 1.219 ± 0.195
1.441PheArg: 1.441 ± 0.167
3.014PheSer: 3.014 ± 0.268
2.881PheThr: 2.881 ± 0.263
2.372PheVal: 2.372 ± 0.249
0.443PheTrp: 0.443 ± 0.109
1.729PheTyr: 1.729 ± 0.234
0.0PheXaa: 0.0 ± 0.0
Gly
3.369GlyAla: 3.369 ± 0.566
0.887GlyCys: 0.887 ± 0.161
3.923GlyAsp: 3.923 ± 0.346
5.009GlyGlu: 5.009 ± 0.362
2.527GlyPhe: 2.527 ± 0.204
4.677GlyGly: 4.677 ± 0.73
1.308GlyHis: 1.308 ± 0.186
4.123GlyIle: 4.123 ± 0.399
4.987GlyLys: 4.987 ± 0.345
4.81GlyLeu: 4.81 ± 0.4
1.529GlyMet: 1.529 ± 0.204
3.657GlyAsn: 3.657 ± 0.26
0.576GlyPro: 0.576 ± 0.13
1.951GlyGln: 1.951 ± 0.225
2.704GlyArg: 2.704 ± 0.244
4.367GlySer: 4.367 ± 0.372
4.078GlyThr: 4.078 ± 0.323
4.655GlyVal: 4.655 ± 0.324
0.909GlyTrp: 0.909 ± 0.16
3.303GlyTyr: 3.303 ± 0.27
0.0GlyXaa: 0.0 ± 0.0
His
1.086HisAla: 1.086 ± 0.143
0.199HisCys: 0.199 ± 0.059
0.82HisAsp: 0.82 ± 0.131
1.042HisGlu: 1.042 ± 0.157
0.687HisPhe: 0.687 ± 0.132
1.086HisGly: 1.086 ± 0.164
0.554HisHis: 0.554 ± 0.123
1.463HisIle: 1.463 ± 0.218
1.241HisLys: 1.241 ± 0.185
1.818HisLeu: 1.818 ± 0.187
0.576HisMet: 0.576 ± 0.096
0.953HisAsn: 0.953 ± 0.153
0.687HisPro: 0.687 ± 0.129
0.51HisGln: 0.51 ± 0.105
0.887HisArg: 0.887 ± 0.148
1.153HisSer: 1.153 ± 0.201
0.953HisThr: 0.953 ± 0.138
1.751HisVal: 1.751 ± 0.198
0.266HisTrp: 0.266 ± 0.086
1.02HisTyr: 1.02 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
3.879IleAla: 3.879 ± 0.291
0.532IleCys: 0.532 ± 0.101
4.588IleAsp: 4.588 ± 0.367
4.81IleGlu: 4.81 ± 0.361
1.84IlePhe: 1.84 ± 0.192
3.524IleGly: 3.524 ± 0.308
1.241IleHis: 1.241 ± 0.171
3.347IleIle: 3.347 ± 0.272
5.054IleLys: 5.054 ± 0.333
4.322IleLeu: 4.322 ± 0.338
1.596IleMet: 1.596 ± 0.169
3.369IleAsn: 3.369 ± 0.296
2.327IlePro: 2.327 ± 0.247
2.527IleGln: 2.527 ± 0.191
2.859IleArg: 2.859 ± 0.261
4.234IleSer: 4.234 ± 0.329
4.256IleThr: 4.256 ± 0.417
4.034IleVal: 4.034 ± 0.29
0.443IleTrp: 0.443 ± 0.096
2.172IleTyr: 2.172 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
5.453LysAla: 5.453 ± 0.364
0.576LysCys: 0.576 ± 0.109
4.921LysAsp: 4.921 ± 0.348
7.713LysGlu: 7.713 ± 0.609
2.793LysPhe: 2.793 ± 0.268
5.142LysGly: 5.142 ± 0.373
1.574LysHis: 1.574 ± 0.193
3.258LysIle: 3.258 ± 0.304
6.893LysLys: 6.893 ± 0.613
5.719LysLeu: 5.719 ± 0.345
2.061LysMet: 2.061 ± 0.222
3.591LysAsn: 3.591 ± 0.306
2.327LysPro: 2.327 ± 0.241
2.992LysGln: 2.992 ± 0.308
3.657LysArg: 3.657 ± 0.318
4.721LysSer: 4.721 ± 0.43
3.812LysThr: 3.812 ± 0.251
4.854LysVal: 4.854 ± 0.313
0.754LysTrp: 0.754 ± 0.11
3.303LysTyr: 3.303 ± 0.279
0.0LysXaa: 0.0 ± 0.0
Leu
4.655LeuAla: 4.655 ± 0.302
0.665LeuCys: 0.665 ± 0.126
6.539LeuAsp: 6.539 ± 0.418
7.248LeuGlu: 7.248 ± 0.506
3.103LeuPhe: 3.103 ± 0.286
4.743LeuGly: 4.743 ± 0.336
1.507LeuHis: 1.507 ± 0.199
4.677LeuIle: 4.677 ± 0.311
6.783LeuLys: 6.783 ± 0.39
6.384LeuLeu: 6.384 ± 0.318
2.084LeuMet: 2.084 ± 0.235
4.721LeuAsn: 4.721 ± 0.317
2.992LeuPro: 2.992 ± 0.264
2.992LeuGln: 2.992 ± 0.273
3.724LeuArg: 3.724 ± 0.31
5.852LeuSer: 5.852 ± 0.369
5.364LeuThr: 5.364 ± 0.379
4.943LeuVal: 4.943 ± 0.346
0.798LeuTrp: 0.798 ± 0.154
3.591LeuTyr: 3.591 ± 0.285
0.0LeuXaa: 0.0 ± 0.0
Met
1.729MetAla: 1.729 ± 0.193
0.133MetCys: 0.133 ± 0.048
1.773MetAsp: 1.773 ± 0.18
2.106MetGlu: 2.106 ± 0.233
1.263MetPhe: 1.263 ± 0.16
1.507MetGly: 1.507 ± 0.211
0.332MetHis: 0.332 ± 0.085
1.419MetIle: 1.419 ± 0.21
2.372MetLys: 2.372 ± 0.23
2.017MetLeu: 2.017 ± 0.22
0.465MetMet: 0.465 ± 0.102
1.596MetAsn: 1.596 ± 0.201
0.776MetPro: 0.776 ± 0.119
0.887MetGln: 0.887 ± 0.142
1.108MetArg: 1.108 ± 0.155
2.106MetSer: 2.106 ± 0.233
2.106MetThr: 2.106 ± 0.196
1.175MetVal: 1.175 ± 0.144
0.199MetTrp: 0.199 ± 0.077
1.13MetTyr: 1.13 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
2.926AsnAla: 2.926 ± 0.312
0.421AsnCys: 0.421 ± 0.098
2.726AsnAsp: 2.726 ± 0.24
3.413AsnGlu: 3.413 ± 0.276
1.685AsnPhe: 1.685 ± 0.194
3.968AsnGly: 3.968 ± 0.357
1.108AsnHis: 1.108 ± 0.16
3.546AsnIle: 3.546 ± 0.283
3.968AsnLys: 3.968 ± 0.285
4.411AsnLeu: 4.411 ± 0.315
1.175AsnMet: 1.175 ± 0.204
3.28AsnAsn: 3.28 ± 0.285
2.571AsnPro: 2.571 ± 0.338
1.685AsnGln: 1.685 ± 0.187
2.682AsnArg: 2.682 ± 0.269
3.214AsnSer: 3.214 ± 0.284
3.192AsnThr: 3.192 ± 0.273
3.413AsnVal: 3.413 ± 0.228
0.554AsnTrp: 0.554 ± 0.107
2.084AsnTyr: 2.084 ± 0.233
0.0AsnXaa: 0.0 ± 0.0
Pro
2.482ProAla: 2.482 ± 0.348
0.244ProCys: 0.244 ± 0.069
2.305ProAsp: 2.305 ± 0.205
3.059ProGlu: 3.059 ± 0.302
1.286ProPhe: 1.286 ± 0.197
1.042ProGly: 1.042 ± 0.143
0.709ProHis: 0.709 ± 0.129
1.84ProIle: 1.84 ± 0.203
2.217ProLys: 2.217 ± 0.266
2.593ProLeu: 2.593 ± 0.25
0.598ProMet: 0.598 ± 0.121
1.795ProAsn: 1.795 ± 0.199
0.975ProPro: 0.975 ± 0.147
1.263ProGln: 1.263 ± 0.178
1.153ProArg: 1.153 ± 0.151
2.017ProSer: 2.017 ± 0.242
2.549ProThr: 2.549 ± 0.261
2.349ProVal: 2.349 ± 0.243
0.199ProTrp: 0.199 ± 0.069
1.529ProTyr: 1.529 ± 0.187
0.0ProXaa: 0.0 ± 0.0
Gln
2.172GlnAla: 2.172 ± 0.262
0.222GlnCys: 0.222 ± 0.066
1.951GlnAsp: 1.951 ± 0.187
3.502GlnGlu: 3.502 ± 0.301
1.33GlnPhe: 1.33 ± 0.178
1.707GlnGly: 1.707 ± 0.193
0.532GlnHis: 0.532 ± 0.115
2.039GlnIle: 2.039 ± 0.21
2.615GlnLys: 2.615 ± 0.229
3.214GlnLeu: 3.214 ± 0.279
1.419GlnMet: 1.419 ± 0.169
2.039GlnAsn: 2.039 ± 0.222
1.108GlnPro: 1.108 ± 0.245
1.928GlnGln: 1.928 ± 0.288
2.106GlnArg: 2.106 ± 0.196
2.239GlnSer: 2.239 ± 0.228
1.552GlnThr: 1.552 ± 0.191
2.815GlnVal: 2.815 ± 0.25
0.332GlnTrp: 0.332 ± 0.079
1.374GlnTyr: 1.374 ± 0.174
0.0GlnXaa: 0.0 ± 0.0
Arg
2.305ArgAla: 2.305 ± 0.235
0.31ArgCys: 0.31 ± 0.102
2.638ArgAsp: 2.638 ± 0.277
3.968ArgGlu: 3.968 ± 0.253
2.017ArgPhe: 2.017 ± 0.217
3.236ArgGly: 3.236 ± 0.293
0.665ArgHis: 0.665 ± 0.141
2.926ArgIle: 2.926 ± 0.272
3.879ArgLys: 3.879 ± 0.352
4.278ArgLeu: 4.278 ± 0.289
1.286ArgMet: 1.286 ± 0.168
2.017ArgAsn: 2.017 ± 0.265
1.13ArgPro: 1.13 ± 0.15
1.707ArgGln: 1.707 ± 0.203
2.061ArgArg: 2.061 ± 0.187
2.217ArgSer: 2.217 ± 0.217
2.793ArgThr: 2.793 ± 0.278
3.413ArgVal: 3.413 ± 0.248
0.377ArgTrp: 0.377 ± 0.087
2.194ArgTyr: 2.194 ± 0.229
0.0ArgXaa: 0.0 ± 0.0
Ser
3.236SerAla: 3.236 ± 0.295
0.443SerCys: 0.443 ± 0.101
3.546SerAsp: 3.546 ± 0.266
4.522SerGlu: 4.522 ± 0.336
2.926SerPhe: 2.926 ± 0.205
4.278SerGly: 4.278 ± 0.38
1.02SerHis: 1.02 ± 0.147
3.857SerIle: 3.857 ± 0.302
5.364SerLys: 5.364 ± 0.399
5.386SerLeu: 5.386 ± 0.315
1.818SerMet: 1.818 ± 0.204
3.436SerAsn: 3.436 ± 0.364
1.751SerPro: 1.751 ± 0.211
1.973SerGln: 1.973 ± 0.206
2.726SerArg: 2.726 ± 0.275
4.898SerSer: 4.898 ± 0.354
4.123SerThr: 4.123 ± 0.405
4.389SerVal: 4.389 ± 0.268
0.776SerTrp: 0.776 ± 0.141
2.748SerTyr: 2.748 ± 0.235
0.0SerXaa: 0.0 ± 0.0
Thr
3.879ThrAla: 3.879 ± 0.409
0.399ThrCys: 0.399 ± 0.093
4.278ThrAsp: 4.278 ± 0.413
4.477ThrGlu: 4.477 ± 0.322
2.283ThrPhe: 2.283 ± 0.227
4.699ThrGly: 4.699 ± 0.369
1.042ThrHis: 1.042 ± 0.166
3.679ThrIle: 3.679 ± 0.354
3.923ThrLys: 3.923 ± 0.27
5.43ThrLeu: 5.43 ± 0.371
1.153ThrMet: 1.153 ± 0.14
2.904ThrAsn: 2.904 ± 0.33
2.593ThrPro: 2.593 ± 0.253
2.349ThrGln: 2.349 ± 0.18
2.859ThrArg: 2.859 ± 0.262
3.325ThrSer: 3.325 ± 0.299
4.145ThrThr: 4.145 ± 0.387
5.076ThrVal: 5.076 ± 0.311
0.554ThrTrp: 0.554 ± 0.115
2.615ThrTyr: 2.615 ± 0.264
0.0ThrXaa: 0.0 ± 0.0
Val
4.078ValAla: 4.078 ± 0.266
0.554ValCys: 0.554 ± 0.138
4.987ValAsp: 4.987 ± 0.339
5.497ValGlu: 5.497 ± 0.353
2.859ValPhe: 2.859 ± 0.253
3.901ValGly: 3.901 ± 0.3
1.352ValHis: 1.352 ± 0.208
3.657ValIle: 3.657 ± 0.291
4.898ValLys: 4.898 ± 0.359
4.921ValLeu: 4.921 ± 0.336
1.574ValMet: 1.574 ± 0.195
3.657ValAsn: 3.657 ± 0.303
2.992ValPro: 2.992 ± 0.308
2.527ValGln: 2.527 ± 0.228
3.147ValArg: 3.147 ± 0.292
5.187ValSer: 5.187 ± 0.372
4.743ValThr: 4.743 ± 0.406
4.633ValVal: 4.633 ± 0.366
0.665ValTrp: 0.665 ± 0.135
3.303ValTyr: 3.303 ± 0.271
0.0ValXaa: 0.0 ± 0.0
Trp
0.554TrpAla: 0.554 ± 0.105
0.177TrpCys: 0.177 ± 0.074
0.842TrpAsp: 0.842 ± 0.135
1.042TrpGlu: 1.042 ± 0.175
0.51TrpPhe: 0.51 ± 0.115
0.643TrpGly: 0.643 ± 0.118
0.222TrpHis: 0.222 ± 0.077
0.554TrpIle: 0.554 ± 0.102
0.842TrpLys: 0.842 ± 0.119
0.709TrpLeu: 0.709 ± 0.131
0.133TrpMet: 0.133 ± 0.056
0.465TrpAsn: 0.465 ± 0.115
0.0TrpPro: 0.0 ± 0.0
0.332TrpGln: 0.332 ± 0.085
0.377TrpArg: 0.377 ± 0.095
0.598TrpSer: 0.598 ± 0.122
0.554TrpThr: 0.554 ± 0.083
0.975TrpVal: 0.975 ± 0.153
0.177TrpTrp: 0.177 ± 0.084
0.687TrpTyr: 0.687 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.638TyrAla: 2.638 ± 0.215
0.332TyrCys: 0.332 ± 0.105
3.369TyrAsp: 3.369 ± 0.269
3.369TyrGlu: 3.369 ± 0.261
1.64TyrPhe: 1.64 ± 0.204
2.881TyrGly: 2.881 ± 0.263
0.909TyrHis: 0.909 ± 0.142
3.037TyrIle: 3.037 ± 0.245
2.926TyrLys: 2.926 ± 0.266
4.588TyrLeu: 4.588 ± 0.33
0.953TyrMet: 0.953 ± 0.148
2.505TyrAsn: 2.505 ± 0.235
1.263TyrPro: 1.263 ± 0.165
1.685TyrGln: 1.685 ± 0.188
2.571TyrArg: 2.571 ± 0.216
2.704TyrSer: 2.704 ± 0.26
2.793TyrThr: 2.793 ± 0.268
2.482TyrVal: 2.482 ± 0.22
0.31TyrTrp: 0.31 ± 0.083
1.84TyrTyr: 1.84 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 236 proteins (45117 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski