Vibrio phage pre-CTX

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7F140|A0A0N7F140_9VIRU Core encoded pilin OS=Vibrio phage pre-CTX OX=689414 GN=cep PE=4 SV=1
MM1 pKa = 7.31MLMMDD6 pKa = 4.7TLYY9 pKa = 11.2DD10 pKa = 3.44WLIDD14 pKa = 3.64GFTWLVIKK22 pKa = 10.51LGIMWIEE29 pKa = 3.84SKK31 pKa = 10.71IFVIQFFWEE40 pKa = 4.3MSQKK44 pKa = 10.43VIDD47 pKa = 4.21MFTIYY52 pKa = 10.39PLIQQAIDD60 pKa = 3.75MLPPQYY66 pKa = 10.95SGFLFFLGLDD76 pKa = 3.32QALAIVLQALMTRR89 pKa = 11.84FALRR93 pKa = 11.84ALNLL97 pKa = 3.56

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142I6Y0|A0A142I6Y0_9VIRU Cholera toxin subunit A OS=Vibrio phage pre-CTX OX=689414 GN=ctxA PE=4 SV=1
MM1 pKa = 7.64FSSLKK6 pKa = 10.78NKK8 pKa = 10.41LNTFKK13 pKa = 10.35STLSLGVFLLFSAFANQALAAADD36 pKa = 3.71AGLVTEE42 pKa = 4.41VTKK45 pKa = 10.35TLGTSKK51 pKa = 10.11DD52 pKa = 3.48TVIALGPLIMGVVGAIVLIVTVIGLIRR79 pKa = 11.84KK80 pKa = 8.93AKK82 pKa = 9.89

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1387

82

427

231.2

25.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.354 ± 0.745

1.514 ± 0.519

5.624 ± 0.517

4.686 ± 0.594

5.84 ± 0.538

6.561 ± 0.595

2.884 ± 0.59

6.2 ± 0.823

4.686 ± 0.785

9.589 ± 1.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.1 ± 0.629

4.254 ± 0.515

4.758 ± 0.537

3.605 ± 0.726

4.614 ± 0.555

7.714 ± 1.144

5.984 ± 0.822

5.84 ± 0.413

1.442 ± 0.366

3.749 ± 0.73
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski