Eimeria stiedai RNA virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1C9C4J3|A0A1C9C4J3_9VIRU Coat protein OS=Eimeria stiedai RNA virus 1 OX=1898175 GN=EsRv1gp1 PE=4 SV=1
MM1 pKa = 7.89AEE3 pKa = 4.18RR4 pKa = 11.84LSGLSAPAQEE14 pKa = 4.38VTSICSNRR22 pKa = 11.84FTSPPKK28 pKa = 9.17TGEE31 pKa = 3.83CRR33 pKa = 11.84VYY35 pKa = 10.87QGMLEE40 pKa = 4.45LSWHH44 pKa = 5.89AWGKK48 pKa = 8.86VKK50 pKa = 10.85RR51 pKa = 11.84NDD53 pKa = 3.09KK54 pKa = 10.36LIRR57 pKa = 11.84YY58 pKa = 7.39EE59 pKa = 3.96VGCRR63 pKa = 11.84FDD65 pKa = 3.3ATGEE69 pKa = 4.11RR70 pKa = 11.84KK71 pKa = 10.04DD72 pKa = 3.76RR73 pKa = 11.84EE74 pKa = 4.03LTIGKK79 pKa = 9.42KK80 pKa = 5.82VTKK83 pKa = 9.86SILGGYY89 pKa = 7.51PFPTLALFMPADD101 pKa = 3.65YY102 pKa = 10.56QEE104 pKa = 4.12RR105 pKa = 11.84LLRR108 pKa = 11.84VEE110 pKa = 4.35PRR112 pKa = 11.84HH113 pKa = 4.68TARR116 pKa = 11.84PNWEE120 pKa = 4.35CFDD123 pKa = 3.58FTGPVAMLAAWLSALTWIPHH143 pKa = 6.21AATSHH148 pKa = 5.97FVDD151 pKa = 3.92TSSISLVPITNLEE164 pKa = 3.98DD165 pKa = 3.79SAALSQNNVFISQNVCGRR183 pKa = 11.84GGSGAYY189 pKa = 7.17WALVAACSACGATVATDD206 pKa = 3.54RR207 pKa = 11.84VIMAGPDD214 pKa = 3.28TPRR217 pKa = 11.84FYY219 pKa = 11.16SAAGYY224 pKa = 9.49HH225 pKa = 6.38LWRR228 pKa = 11.84DD229 pKa = 3.4VLDD232 pKa = 3.84ALRR235 pKa = 11.84ILGEE239 pKa = 4.15LYY241 pKa = 10.65DD242 pKa = 3.73RR243 pKa = 11.84AGGGEE248 pKa = 4.02VFAYY252 pKa = 10.35AFTKK256 pKa = 10.53GIHH259 pKa = 5.7SVSTVVAHH267 pKa = 6.45SDD269 pKa = 3.12EE270 pKa = 4.88GGLMRR275 pKa = 11.84DD276 pKa = 3.11ILRR279 pKa = 11.84EE280 pKa = 3.67RR281 pKa = 11.84RR282 pKa = 11.84FAIPFGGLPAGCGFHH297 pKa = 7.24PGMPTLAKK305 pKa = 9.62GAEE308 pKa = 4.01YY309 pKa = 10.41RR310 pKa = 11.84HH311 pKa = 6.24LARR314 pKa = 11.84LVDD317 pKa = 4.67AYY319 pKa = 11.36LLQTAAAVAHH329 pKa = 6.48CAPLVYY335 pKa = 9.96TGLGTSCPSVAYY347 pKa = 10.16GRR349 pKa = 11.84LAPEE353 pKa = 3.93YY354 pKa = 8.51QHH356 pKa = 5.92QKK358 pKa = 10.13RR359 pKa = 11.84MEE361 pKa = 4.34DD362 pKa = 3.49FEE364 pKa = 5.27TPFLDD369 pKa = 4.37ASTHH373 pKa = 5.7AKK375 pKa = 10.32DD376 pKa = 3.63VVSARR381 pKa = 11.84TQHH384 pKa = 6.51VEE386 pKa = 3.45ILRR389 pKa = 11.84QALEE393 pKa = 3.7HH394 pKa = 6.56SFFEE398 pKa = 4.88FGALYY403 pKa = 10.49LPAIASFWGFSPTGEE418 pKa = 3.94RR419 pKa = 11.84EE420 pKa = 3.94QACRR424 pKa = 11.84QWPWDD429 pKa = 3.59ALGSVLDD436 pKa = 4.33DD437 pKa = 3.35CHH439 pKa = 6.69NRR441 pKa = 11.84HH442 pKa = 6.37LIKK445 pKa = 10.8GEE447 pKa = 4.09SIAPFFWIEE456 pKa = 3.79PTSILPHH463 pKa = 4.65SWRR466 pKa = 11.84RR467 pKa = 11.84TACEE471 pKa = 3.7TDD473 pKa = 2.83GWASMGGPGEE483 pKa = 4.06SRR485 pKa = 11.84RR486 pKa = 11.84IRR488 pKa = 11.84MFPQILEE495 pKa = 4.75DD496 pKa = 4.07PDD498 pKa = 4.27SSFTHH503 pKa = 7.18RR504 pKa = 11.84YY505 pKa = 8.57YY506 pKa = 11.58LLGFQGARR514 pKa = 11.84KK515 pKa = 9.77CPWLWALNGHH525 pKa = 6.38RR526 pKa = 11.84EE527 pKa = 4.13DD528 pKa = 4.36GLAQVRR534 pKa = 11.84LRR536 pKa = 11.84VAYY539 pKa = 9.69PEE541 pKa = 5.14DD542 pKa = 3.68FLNLGGRR549 pKa = 11.84AAKK552 pKa = 9.8EE553 pKa = 3.82GPTAVFSEE561 pKa = 4.21QAPISDD567 pKa = 4.24YY568 pKa = 11.35LWVRR572 pKa = 11.84GQNPLPHH579 pKa = 6.76PAEE582 pKa = 4.02FLNTGEE588 pKa = 4.39TIGVEE593 pKa = 3.92IKK595 pKa = 10.87HH596 pKa = 6.29FDD598 pKa = 3.58LDD600 pKa = 3.52KK601 pKa = 11.4DD602 pKa = 3.48HH603 pKa = 7.2WLKK606 pKa = 11.36AMPSPNEE613 pKa = 3.38IRR615 pKa = 11.84EE616 pKa = 4.19AVLDD620 pKa = 4.04LVVSAPQGLRR630 pKa = 11.84VGKK633 pKa = 10.58SNTEE637 pKa = 3.54TRR639 pKa = 11.84QEE641 pKa = 3.46IGYY644 pKa = 8.03RR645 pKa = 11.84TAGARR650 pKa = 11.84AMHH653 pKa = 6.72RR654 pKa = 11.84ALAEE658 pKa = 3.96VAADD662 pKa = 3.57MQSRR666 pKa = 11.84PTVGEE671 pKa = 3.59ARR673 pKa = 11.84IEE675 pKa = 3.86RR676 pKa = 11.84LPAYY680 pKa = 8.21FLPGGGGGTGTVTVSRR696 pKa = 11.84GGLTSAPMDD705 pKa = 3.95TAPGDD710 pKa = 3.7AEE712 pKa = 4.29RR713 pKa = 11.84PTGGGPPTASVDD725 pKa = 3.53VGTVRR730 pKa = 11.84AVHH733 pKa = 6.9AKK735 pKa = 10.06RR736 pKa = 11.84LAPTLAAGTHH746 pKa = 5.41TGPRR750 pKa = 11.84LPHH753 pKa = 5.91PQAVSGGPAPMGPTATPPARR773 pKa = 11.84PGDD776 pKa = 3.95DD777 pKa = 3.4STADD781 pKa = 3.37FCGGQDD787 pKa = 3.21GGAATGTAPQAPP799 pKa = 4.03

Molecular weight:
86.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1C9C4J3|A0A1C9C4J3_9VIRU Coat protein OS=Eimeria stiedai RNA virus 1 OX=1898175 GN=EsRv1gp1 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.12RR3 pKa = 11.84VGGAASRR10 pKa = 11.84GALRR14 pKa = 11.84EE15 pKa = 4.11AADD18 pKa = 3.61SCGLTRR24 pKa = 11.84LLVGFIAEE32 pKa = 4.36ASALSRR38 pKa = 11.84LPEE41 pKa = 3.96SEE43 pKa = 4.26RR44 pKa = 11.84YY45 pKa = 8.92GAIISACGGGAEE57 pKa = 4.92DD58 pKa = 4.13RR59 pKa = 11.84LEE61 pKa = 4.42ACVCYY66 pKa = 10.63NILSNPTPIHH76 pKa = 6.41LPQPAAWVWPILLGVGEE93 pKa = 4.31AAGRR97 pKa = 11.84MLPPEE102 pKa = 4.2WRR104 pKa = 11.84RR105 pKa = 11.84CIAMLPRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84YY115 pKa = 9.52SVLPRR120 pKa = 11.84AQGNRR125 pKa = 11.84WLGSSKK131 pKa = 10.63GRR133 pKa = 11.84GVDD136 pKa = 3.59SLASSYY142 pKa = 11.23ALHH145 pKa = 6.53VDD147 pKa = 2.9SHH149 pKa = 6.73GRR151 pKa = 11.84DD152 pKa = 3.35GKK154 pKa = 9.72PALGALCRR162 pKa = 11.84YY163 pKa = 9.79LSEE166 pKa = 5.23ADD168 pKa = 3.47AAIHH172 pKa = 5.87EE173 pKa = 4.65GTRR176 pKa = 11.84RR177 pKa = 11.84VLASRR182 pKa = 11.84AVPPAASVSGLLASGRR198 pKa = 11.84EE199 pKa = 4.0LFEE202 pKa = 6.47AGLLGEE208 pKa = 4.45EE209 pKa = 4.56QDD211 pKa = 3.8TEE213 pKa = 3.93AHH215 pKa = 5.73VRR217 pKa = 11.84VDD219 pKa = 3.69GPSPRR224 pKa = 11.84MRR226 pKa = 11.84ARR228 pKa = 11.84RR229 pKa = 11.84GGGGSYY235 pKa = 9.78VAPGISRR242 pKa = 11.84VASRR246 pKa = 11.84LATSVRR252 pKa = 11.84GGVNSLLTWLRR263 pKa = 11.84DD264 pKa = 3.79FSVSEE269 pKa = 3.91VAGALGRR276 pKa = 11.84VATQRR281 pKa = 11.84LVGASAGPAVLEE293 pKa = 3.95RR294 pKa = 11.84RR295 pKa = 11.84LEE297 pKa = 3.96EE298 pKa = 3.82RR299 pKa = 11.84ARR301 pKa = 11.84PSPVSSGVTSGRR313 pKa = 11.84PWARR317 pKa = 11.84GLDD320 pKa = 3.9DD321 pKa = 4.96KK322 pKa = 11.72GGSCGVLGGSGWSMKK337 pKa = 9.7VSRR340 pKa = 11.84RR341 pKa = 11.84QAEE344 pKa = 3.96GLMRR348 pKa = 11.84SISLPEE354 pKa = 3.62IALTLVKK361 pKa = 10.38RR362 pKa = 11.84EE363 pKa = 3.69AGLRR367 pKa = 11.84LRR369 pKa = 11.84SRR371 pKa = 11.84LQARR375 pKa = 11.84FPRR378 pKa = 11.84KK379 pKa = 9.57QMSQATSKK387 pKa = 11.24VNVFLDD393 pKa = 3.76SLLMDD398 pKa = 4.69LAAYY402 pKa = 9.72DD403 pKa = 3.77PTGCSLSLSILDD415 pKa = 3.79TVPAGTLYY423 pKa = 10.73EE424 pKa = 4.28DD425 pKa = 3.95QVCGAVLHH433 pKa = 6.52AFSLCHH439 pKa = 5.61GRR441 pKa = 11.84AGATLLLILVLGLAAGGTPLQGLSDD466 pKa = 3.83VLKK469 pKa = 9.82ATGSNTSFEE478 pKa = 4.21WAHH481 pKa = 5.92FCEE484 pKa = 4.45LHH486 pKa = 6.36CLLGRR491 pKa = 11.84GAGEE495 pKa = 4.52LDD497 pKa = 3.76LKK499 pKa = 10.8EE500 pKa = 4.23DD501 pKa = 3.22AARR504 pKa = 11.84RR505 pKa = 11.84CRR507 pKa = 11.84PKK509 pKa = 9.99PDD511 pKa = 3.37MEE513 pKa = 4.47VEE515 pKa = 3.83VDD517 pKa = 3.67PVKK520 pKa = 11.17LEE522 pKa = 3.98TAVRR526 pKa = 11.84AVLAEE531 pKa = 3.95EE532 pKa = 5.16LGTDD536 pKa = 3.68NLIIDD541 pKa = 4.67DD542 pKa = 5.47LDD544 pKa = 3.79RR545 pKa = 11.84HH546 pKa = 5.19WDD548 pKa = 4.12RR549 pKa = 11.84RR550 pKa = 11.84FEE552 pKa = 3.97WCVAGAHH559 pKa = 6.1AASVNQCGGFSCVPSCGPGGRR580 pKa = 11.84KK581 pKa = 5.54MTRR584 pKa = 11.84RR585 pKa = 11.84MAMEE589 pKa = 3.99YY590 pKa = 10.83VEE592 pKa = 5.34GNPLHH597 pKa = 6.65SWDD600 pKa = 4.23GEE602 pKa = 4.57VKK604 pKa = 10.65VSVIPKK610 pKa = 9.6LEE612 pKa = 4.16HH613 pKa = 5.38GKK615 pKa = 9.46IRR617 pKa = 11.84AIYY620 pKa = 8.39SCNTISYY627 pKa = 9.84AIFSRR632 pKa = 11.84VLRR635 pKa = 11.84PAEE638 pKa = 4.07RR639 pKa = 11.84KK640 pKa = 6.78WAGRR644 pKa = 11.84RR645 pKa = 11.84VIVDD649 pKa = 3.73PGAGGNYY656 pKa = 10.43GMFKK660 pKa = 10.33RR661 pKa = 11.84IRR663 pKa = 11.84TAWPRR668 pKa = 11.84SLPVAVMLDD677 pKa = 3.58YY678 pKa = 11.67ADD680 pKa = 5.2FNSQHH685 pKa = 5.92TLGAQQTVIRR695 pKa = 11.84CLLEE699 pKa = 3.93RR700 pKa = 11.84CPGISAAEE708 pKa = 4.29KK709 pKa = 8.27EE710 pKa = 4.53TMIASFDD717 pKa = 3.12RR718 pKa = 11.84MYY720 pKa = 10.28IYY722 pKa = 10.59HH723 pKa = 6.7SGALVGRR730 pKa = 11.84CRR732 pKa = 11.84RR733 pKa = 11.84SLMSGHH739 pKa = 7.06RR740 pKa = 11.84GTSFINSVLNAAYY753 pKa = 10.29LRR755 pKa = 11.84VVLGEE760 pKa = 4.26DD761 pKa = 4.86GYY763 pKa = 12.08AKK765 pKa = 10.36LQSFHH770 pKa = 8.14VGDD773 pKa = 4.44DD774 pKa = 3.42VLIFARR780 pKa = 11.84DD781 pKa = 3.34EE782 pKa = 4.1RR783 pKa = 11.84SGYY786 pKa = 10.48EE787 pKa = 3.78IIQAMTAAGFHH798 pKa = 5.98LQAKK802 pKa = 7.78KK803 pKa = 10.56QSVGRR808 pKa = 11.84AGFEE812 pKa = 4.19FLRR815 pKa = 11.84MAGTRR820 pKa = 11.84RR821 pKa = 11.84AARR824 pKa = 11.84GYY826 pKa = 9.77LARR829 pKa = 11.84SVASTVSGSWTSDD842 pKa = 2.73EE843 pKa = 4.15RR844 pKa = 11.84LGAIASLHH852 pKa = 5.96NAVQQARR859 pKa = 11.84SLINRR864 pKa = 11.84SGNMDD869 pKa = 3.88CYY871 pKa = 11.31KK872 pKa = 10.85LLLRR876 pKa = 11.84SAYY879 pKa = 10.37ARR881 pKa = 11.84TSLPLSILGEE891 pKa = 4.16VLSGQVAVGAGPVYY905 pKa = 10.4RR906 pKa = 11.84ADD908 pKa = 2.66GHH910 pKa = 5.71YY911 pKa = 10.48YY912 pKa = 9.51NRR914 pKa = 11.84EE915 pKa = 3.86VLEE918 pKa = 4.29LDD920 pKa = 4.18LARR923 pKa = 11.84EE924 pKa = 4.14HH925 pKa = 6.91HH926 pKa = 7.0VDD928 pKa = 4.07SIPWRR933 pKa = 11.84KK934 pKa = 8.75LARR937 pKa = 11.84HH938 pKa = 5.36ATSDD942 pKa = 3.29YY943 pKa = 9.9FARR946 pKa = 11.84GRR948 pKa = 11.84ADD950 pKa = 3.62LEE952 pKa = 3.96EE953 pKa = 4.58HH954 pKa = 6.99AMFLVGYY961 pKa = 8.81KK962 pKa = 9.76PWLAALRR969 pKa = 11.84STYY972 pKa = 11.09GDD974 pKa = 3.27LAGVLNEE981 pKa = 4.94PGPDD985 pKa = 4.23DD986 pKa = 6.0GDD988 pKa = 3.62DD989 pKa = 3.64TPRR992 pKa = 11.84AVALGVKK999 pKa = 10.25RR1000 pKa = 11.84MFTKK1004 pKa = 10.54SGVVSLDD1011 pKa = 3.38TEE1013 pKa = 4.19LDD1015 pKa = 3.6KK1016 pKa = 11.06PVQRR1020 pKa = 11.84GLLVHH1025 pKa = 5.96YY1026 pKa = 8.04PIIVLLQHH1034 pKa = 6.69SLDD1037 pKa = 3.71NNQIGEE1043 pKa = 4.34LLRR1046 pKa = 11.84AAGHH1050 pKa = 5.93LCEE1053 pKa = 5.36PGAEE1057 pKa = 3.86RR1058 pKa = 11.84IAAFGDD1064 pKa = 3.65VKK1066 pKa = 10.74LGSVINGWLPYY1077 pKa = 10.36SDD1079 pKa = 4.55AAALGARR1086 pKa = 11.84GLWQTVRR1093 pKa = 11.84VLYY1096 pKa = 8.93PLRR1099 pKa = 11.84MM1100 pKa = 3.89

Molecular weight:
118.87 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1899

799

1100

949.5

102.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.69 ± 0.111

2.001 ± 0.069

4.792 ± 0.05

5.319 ± 0.105

2.949 ± 0.59

10.321 ± 0.103

2.791 ± 0.259

3.476 ± 0.054

2.58 ± 0.043

10.216 ± 1.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.001 ± 0.069

1.896 ± 0.08

5.74 ± 0.988

2.528 ± 0.196

8.636 ± 0.769

7.162 ± 0.714

4.739 ± 1.058

6.635 ± 0.56

1.843 ± 0.159

2.686 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski