Eimeria stiedai RNA virus 1
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C9C4J3|A0A1C9C4J3_9VIRU Coat protein OS=Eimeria stiedai RNA virus 1 OX=1898175 GN=EsRv1gp1 PE=4 SV=1
MM1 pKa = 7.89 AEE3 pKa = 4.18 RR4 pKa = 11.84 LSGLSAPAQEE14 pKa = 4.38 VTSICSNRR22 pKa = 11.84 FTSPPKK28 pKa = 9.17 TGEE31 pKa = 3.83 CRR33 pKa = 11.84 VYY35 pKa = 10.87 QGMLEE40 pKa = 4.45 LSWHH44 pKa = 5.89 AWGKK48 pKa = 8.86 VKK50 pKa = 10.85 RR51 pKa = 11.84 NDD53 pKa = 3.09 KK54 pKa = 10.36 LIRR57 pKa = 11.84 YY58 pKa = 7.39 EE59 pKa = 3.96 VGCRR63 pKa = 11.84 FDD65 pKa = 3.3 ATGEE69 pKa = 4.11 RR70 pKa = 11.84 KK71 pKa = 10.04 DD72 pKa = 3.76 RR73 pKa = 11.84 EE74 pKa = 4.03 LTIGKK79 pKa = 9.42 KK80 pKa = 5.82 VTKK83 pKa = 9.86 SILGGYY89 pKa = 7.51 PFPTLALFMPADD101 pKa = 3.65 YY102 pKa = 10.56 QEE104 pKa = 4.12 RR105 pKa = 11.84 LLRR108 pKa = 11.84 VEE110 pKa = 4.35 PRR112 pKa = 11.84 HH113 pKa = 4.68 TARR116 pKa = 11.84 PNWEE120 pKa = 4.35 CFDD123 pKa = 3.58 FTGPVAMLAAWLSALTWIPHH143 pKa = 6.21 AATSHH148 pKa = 5.97 FVDD151 pKa = 3.92 TSSISLVPITNLEE164 pKa = 3.98 DD165 pKa = 3.79 SAALSQNNVFISQNVCGRR183 pKa = 11.84 GGSGAYY189 pKa = 7.17 WALVAACSACGATVATDD206 pKa = 3.54 RR207 pKa = 11.84 VIMAGPDD214 pKa = 3.28 TPRR217 pKa = 11.84 FYY219 pKa = 11.16 SAAGYY224 pKa = 9.49 HH225 pKa = 6.38 LWRR228 pKa = 11.84 DD229 pKa = 3.4 VLDD232 pKa = 3.84 ALRR235 pKa = 11.84 ILGEE239 pKa = 4.15 LYY241 pKa = 10.65 DD242 pKa = 3.73 RR243 pKa = 11.84 AGGGEE248 pKa = 4.02 VFAYY252 pKa = 10.35 AFTKK256 pKa = 10.53 GIHH259 pKa = 5.7 SVSTVVAHH267 pKa = 6.45 SDD269 pKa = 3.12 EE270 pKa = 4.88 GGLMRR275 pKa = 11.84 DD276 pKa = 3.11 ILRR279 pKa = 11.84 EE280 pKa = 3.67 RR281 pKa = 11.84 RR282 pKa = 11.84 FAIPFGGLPAGCGFHH297 pKa = 7.24 PGMPTLAKK305 pKa = 9.62 GAEE308 pKa = 4.01 YY309 pKa = 10.41 RR310 pKa = 11.84 HH311 pKa = 6.24 LARR314 pKa = 11.84 LVDD317 pKa = 4.67 AYY319 pKa = 11.36 LLQTAAAVAHH329 pKa = 6.48 CAPLVYY335 pKa = 9.96 TGLGTSCPSVAYY347 pKa = 10.16 GRR349 pKa = 11.84 LAPEE353 pKa = 3.93 YY354 pKa = 8.51 QHH356 pKa = 5.92 QKK358 pKa = 10.13 RR359 pKa = 11.84 MEE361 pKa = 4.34 DD362 pKa = 3.49 FEE364 pKa = 5.27 TPFLDD369 pKa = 4.37 ASTHH373 pKa = 5.7 AKK375 pKa = 10.32 DD376 pKa = 3.63 VVSARR381 pKa = 11.84 TQHH384 pKa = 6.51 VEE386 pKa = 3.45 ILRR389 pKa = 11.84 QALEE393 pKa = 3.7 HH394 pKa = 6.56 SFFEE398 pKa = 4.88 FGALYY403 pKa = 10.49 LPAIASFWGFSPTGEE418 pKa = 3.94 RR419 pKa = 11.84 EE420 pKa = 3.94 QACRR424 pKa = 11.84 QWPWDD429 pKa = 3.59 ALGSVLDD436 pKa = 4.33 DD437 pKa = 3.35 CHH439 pKa = 6.69 NRR441 pKa = 11.84 HH442 pKa = 6.37 LIKK445 pKa = 10.8 GEE447 pKa = 4.09 SIAPFFWIEE456 pKa = 3.79 PTSILPHH463 pKa = 4.65 SWRR466 pKa = 11.84 RR467 pKa = 11.84 TACEE471 pKa = 3.7 TDD473 pKa = 2.83 GWASMGGPGEE483 pKa = 4.06 SRR485 pKa = 11.84 RR486 pKa = 11.84 IRR488 pKa = 11.84 MFPQILEE495 pKa = 4.75 DD496 pKa = 4.07 PDD498 pKa = 4.27 SSFTHH503 pKa = 7.18 RR504 pKa = 11.84 YY505 pKa = 8.57 YY506 pKa = 11.58 LLGFQGARR514 pKa = 11.84 KK515 pKa = 9.77 CPWLWALNGHH525 pKa = 6.38 RR526 pKa = 11.84 EE527 pKa = 4.13 DD528 pKa = 4.36 GLAQVRR534 pKa = 11.84 LRR536 pKa = 11.84 VAYY539 pKa = 9.69 PEE541 pKa = 5.14 DD542 pKa = 3.68 FLNLGGRR549 pKa = 11.84 AAKK552 pKa = 9.8 EE553 pKa = 3.82 GPTAVFSEE561 pKa = 4.21 QAPISDD567 pKa = 4.24 YY568 pKa = 11.35 LWVRR572 pKa = 11.84 GQNPLPHH579 pKa = 6.76 PAEE582 pKa = 4.02 FLNTGEE588 pKa = 4.39 TIGVEE593 pKa = 3.92 IKK595 pKa = 10.87 HH596 pKa = 6.29 FDD598 pKa = 3.58 LDD600 pKa = 3.52 KK601 pKa = 11.4 DD602 pKa = 3.48 HH603 pKa = 7.2 WLKK606 pKa = 11.36 AMPSPNEE613 pKa = 3.38 IRR615 pKa = 11.84 EE616 pKa = 4.19 AVLDD620 pKa = 4.04 LVVSAPQGLRR630 pKa = 11.84 VGKK633 pKa = 10.58 SNTEE637 pKa = 3.54 TRR639 pKa = 11.84 QEE641 pKa = 3.46 IGYY644 pKa = 8.03 RR645 pKa = 11.84 TAGARR650 pKa = 11.84 AMHH653 pKa = 6.72 RR654 pKa = 11.84 ALAEE658 pKa = 3.96 VAADD662 pKa = 3.57 MQSRR666 pKa = 11.84 PTVGEE671 pKa = 3.59 ARR673 pKa = 11.84 IEE675 pKa = 3.86 RR676 pKa = 11.84 LPAYY680 pKa = 8.21 FLPGGGGGTGTVTVSRR696 pKa = 11.84 GGLTSAPMDD705 pKa = 3.95 TAPGDD710 pKa = 3.7 AEE712 pKa = 4.29 RR713 pKa = 11.84 PTGGGPPTASVDD725 pKa = 3.53 VGTVRR730 pKa = 11.84 AVHH733 pKa = 6.9 AKK735 pKa = 10.06 RR736 pKa = 11.84 LAPTLAAGTHH746 pKa = 5.41 TGPRR750 pKa = 11.84 LPHH753 pKa = 5.91 PQAVSGGPAPMGPTATPPARR773 pKa = 11.84 PGDD776 pKa = 3.95 DD777 pKa = 3.4 STADD781 pKa = 3.37 FCGGQDD787 pKa = 3.21 GGAATGTAPQAPP799 pKa = 4.03
Molecular weight: 86.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.23
IPC2_protein 6.173
IPC_protein 6.262
Toseland 6.561
ProMoST 6.693
Dawson 6.62
Bjellqvist 6.605
Wikipedia 6.634
Rodwell 6.62
Grimsley 6.693
Solomon 6.62
Lehninger 6.62
Nozaki 6.912
DTASelect 7.073
Thurlkill 7.102
EMBOSS 7.073
Sillero 7.015
Patrickios 4.215
IPC_peptide 6.634
IPC2_peptide 6.942
IPC2.peptide.svr19 6.829
Protein with the highest isoelectric point:
>tr|A0A1C9C4J3|A0A1C9C4J3_9VIRU Coat protein OS=Eimeria stiedai RNA virus 1 OX=1898175 GN=EsRv1gp1 PE=4 SV=1
MM1 pKa = 7.2 KK2 pKa = 10.12 RR3 pKa = 11.84 VGGAASRR10 pKa = 11.84 GALRR14 pKa = 11.84 EE15 pKa = 4.11 AADD18 pKa = 3.61 SCGLTRR24 pKa = 11.84 LLVGFIAEE32 pKa = 4.36 ASALSRR38 pKa = 11.84 LPEE41 pKa = 3.96 SEE43 pKa = 4.26 RR44 pKa = 11.84 YY45 pKa = 8.92 GAIISACGGGAEE57 pKa = 4.92 DD58 pKa = 4.13 RR59 pKa = 11.84 LEE61 pKa = 4.42 ACVCYY66 pKa = 10.63 NILSNPTPIHH76 pKa = 6.41 LPQPAAWVWPILLGVGEE93 pKa = 4.31 AAGRR97 pKa = 11.84 MLPPEE102 pKa = 4.2 WRR104 pKa = 11.84 RR105 pKa = 11.84 CIAMLPRR112 pKa = 11.84 RR113 pKa = 11.84 RR114 pKa = 11.84 YY115 pKa = 9.52 SVLPRR120 pKa = 11.84 AQGNRR125 pKa = 11.84 WLGSSKK131 pKa = 10.63 GRR133 pKa = 11.84 GVDD136 pKa = 3.59 SLASSYY142 pKa = 11.23 ALHH145 pKa = 6.53 VDD147 pKa = 2.9 SHH149 pKa = 6.73 GRR151 pKa = 11.84 DD152 pKa = 3.35 GKK154 pKa = 9.72 PALGALCRR162 pKa = 11.84 YY163 pKa = 9.79 LSEE166 pKa = 5.23 ADD168 pKa = 3.47 AAIHH172 pKa = 5.87 EE173 pKa = 4.65 GTRR176 pKa = 11.84 RR177 pKa = 11.84 VLASRR182 pKa = 11.84 AVPPAASVSGLLASGRR198 pKa = 11.84 EE199 pKa = 4.0 LFEE202 pKa = 6.47 AGLLGEE208 pKa = 4.45 EE209 pKa = 4.56 QDD211 pKa = 3.8 TEE213 pKa = 3.93 AHH215 pKa = 5.73 VRR217 pKa = 11.84 VDD219 pKa = 3.69 GPSPRR224 pKa = 11.84 MRR226 pKa = 11.84 ARR228 pKa = 11.84 RR229 pKa = 11.84 GGGGSYY235 pKa = 9.78 VAPGISRR242 pKa = 11.84 VASRR246 pKa = 11.84 LATSVRR252 pKa = 11.84 GGVNSLLTWLRR263 pKa = 11.84 DD264 pKa = 3.79 FSVSEE269 pKa = 3.91 VAGALGRR276 pKa = 11.84 VATQRR281 pKa = 11.84 LVGASAGPAVLEE293 pKa = 3.95 RR294 pKa = 11.84 RR295 pKa = 11.84 LEE297 pKa = 3.96 EE298 pKa = 3.82 RR299 pKa = 11.84 ARR301 pKa = 11.84 PSPVSSGVTSGRR313 pKa = 11.84 PWARR317 pKa = 11.84 GLDD320 pKa = 3.9 DD321 pKa = 4.96 KK322 pKa = 11.72 GGSCGVLGGSGWSMKK337 pKa = 9.7 VSRR340 pKa = 11.84 RR341 pKa = 11.84 QAEE344 pKa = 3.96 GLMRR348 pKa = 11.84 SISLPEE354 pKa = 3.62 IALTLVKK361 pKa = 10.38 RR362 pKa = 11.84 EE363 pKa = 3.69 AGLRR367 pKa = 11.84 LRR369 pKa = 11.84 SRR371 pKa = 11.84 LQARR375 pKa = 11.84 FPRR378 pKa = 11.84 KK379 pKa = 9.57 QMSQATSKK387 pKa = 11.24 VNVFLDD393 pKa = 3.76 SLLMDD398 pKa = 4.69 LAAYY402 pKa = 9.72 DD403 pKa = 3.77 PTGCSLSLSILDD415 pKa = 3.79 TVPAGTLYY423 pKa = 10.73 EE424 pKa = 4.28 DD425 pKa = 3.95 QVCGAVLHH433 pKa = 6.52 AFSLCHH439 pKa = 5.61 GRR441 pKa = 11.84 AGATLLLILVLGLAAGGTPLQGLSDD466 pKa = 3.83 VLKK469 pKa = 9.82 ATGSNTSFEE478 pKa = 4.21 WAHH481 pKa = 5.92 FCEE484 pKa = 4.45 LHH486 pKa = 6.36 CLLGRR491 pKa = 11.84 GAGEE495 pKa = 4.52 LDD497 pKa = 3.76 LKK499 pKa = 10.8 EE500 pKa = 4.23 DD501 pKa = 3.22 AARR504 pKa = 11.84 RR505 pKa = 11.84 CRR507 pKa = 11.84 PKK509 pKa = 9.99 PDD511 pKa = 3.37 MEE513 pKa = 4.47 VEE515 pKa = 3.83 VDD517 pKa = 3.67 PVKK520 pKa = 11.17 LEE522 pKa = 3.98 TAVRR526 pKa = 11.84 AVLAEE531 pKa = 3.95 EE532 pKa = 5.16 LGTDD536 pKa = 3.68 NLIIDD541 pKa = 4.67 DD542 pKa = 5.47 LDD544 pKa = 3.79 RR545 pKa = 11.84 HH546 pKa = 5.19 WDD548 pKa = 4.12 RR549 pKa = 11.84 RR550 pKa = 11.84 FEE552 pKa = 3.97 WCVAGAHH559 pKa = 6.1 AASVNQCGGFSCVPSCGPGGRR580 pKa = 11.84 KK581 pKa = 5.54 MTRR584 pKa = 11.84 RR585 pKa = 11.84 MAMEE589 pKa = 3.99 YY590 pKa = 10.83 VEE592 pKa = 5.34 GNPLHH597 pKa = 6.65 SWDD600 pKa = 4.23 GEE602 pKa = 4.57 VKK604 pKa = 10.65 VSVIPKK610 pKa = 9.6 LEE612 pKa = 4.16 HH613 pKa = 5.38 GKK615 pKa = 9.46 IRR617 pKa = 11.84 AIYY620 pKa = 8.39 SCNTISYY627 pKa = 9.84 AIFSRR632 pKa = 11.84 VLRR635 pKa = 11.84 PAEE638 pKa = 4.07 RR639 pKa = 11.84 KK640 pKa = 6.78 WAGRR644 pKa = 11.84 RR645 pKa = 11.84 VIVDD649 pKa = 3.73 PGAGGNYY656 pKa = 10.43 GMFKK660 pKa = 10.33 RR661 pKa = 11.84 IRR663 pKa = 11.84 TAWPRR668 pKa = 11.84 SLPVAVMLDD677 pKa = 3.58 YY678 pKa = 11.67 ADD680 pKa = 5.2 FNSQHH685 pKa = 5.92 TLGAQQTVIRR695 pKa = 11.84 CLLEE699 pKa = 3.93 RR700 pKa = 11.84 CPGISAAEE708 pKa = 4.29 KK709 pKa = 8.27 EE710 pKa = 4.53 TMIASFDD717 pKa = 3.12 RR718 pKa = 11.84 MYY720 pKa = 10.28 IYY722 pKa = 10.59 HH723 pKa = 6.7 SGALVGRR730 pKa = 11.84 CRR732 pKa = 11.84 RR733 pKa = 11.84 SLMSGHH739 pKa = 7.06 RR740 pKa = 11.84 GTSFINSVLNAAYY753 pKa = 10.29 LRR755 pKa = 11.84 VVLGEE760 pKa = 4.26 DD761 pKa = 4.86 GYY763 pKa = 12.08 AKK765 pKa = 10.36 LQSFHH770 pKa = 8.14 VGDD773 pKa = 4.44 DD774 pKa = 3.42 VLIFARR780 pKa = 11.84 DD781 pKa = 3.34 EE782 pKa = 4.1 RR783 pKa = 11.84 SGYY786 pKa = 10.48 EE787 pKa = 3.78 IIQAMTAAGFHH798 pKa = 5.98 LQAKK802 pKa = 7.78 KK803 pKa = 10.56 QSVGRR808 pKa = 11.84 AGFEE812 pKa = 4.19 FLRR815 pKa = 11.84 MAGTRR820 pKa = 11.84 RR821 pKa = 11.84 AARR824 pKa = 11.84 GYY826 pKa = 9.77 LARR829 pKa = 11.84 SVASTVSGSWTSDD842 pKa = 2.73 EE843 pKa = 4.15 RR844 pKa = 11.84 LGAIASLHH852 pKa = 5.96 NAVQQARR859 pKa = 11.84 SLINRR864 pKa = 11.84 SGNMDD869 pKa = 3.88 CYY871 pKa = 11.31 KK872 pKa = 10.85 LLLRR876 pKa = 11.84 SAYY879 pKa = 10.37 ARR881 pKa = 11.84 TSLPLSILGEE891 pKa = 4.16 VLSGQVAVGAGPVYY905 pKa = 10.4 RR906 pKa = 11.84 ADD908 pKa = 2.66 GHH910 pKa = 5.71 YY911 pKa = 10.48 YY912 pKa = 9.51 NRR914 pKa = 11.84 EE915 pKa = 3.86 VLEE918 pKa = 4.29 LDD920 pKa = 4.18 LARR923 pKa = 11.84 EE924 pKa = 4.14 HH925 pKa = 6.91 HH926 pKa = 7.0 VDD928 pKa = 4.07 SIPWRR933 pKa = 11.84 KK934 pKa = 8.75 LARR937 pKa = 11.84 HH938 pKa = 5.36 ATSDD942 pKa = 3.29 YY943 pKa = 9.9 FARR946 pKa = 11.84 GRR948 pKa = 11.84 ADD950 pKa = 3.62 LEE952 pKa = 3.96 EE953 pKa = 4.58 HH954 pKa = 6.99 AMFLVGYY961 pKa = 8.81 KK962 pKa = 9.76 PWLAALRR969 pKa = 11.84 STYY972 pKa = 11.09 GDD974 pKa = 3.27 LAGVLNEE981 pKa = 4.94 PGPDD985 pKa = 4.23 DD986 pKa = 6.0 GDD988 pKa = 3.62 DD989 pKa = 3.64 TPRR992 pKa = 11.84 AVALGVKK999 pKa = 10.25 RR1000 pKa = 11.84 MFTKK1004 pKa = 10.54 SGVVSLDD1011 pKa = 3.38 TEE1013 pKa = 4.19 LDD1015 pKa = 3.6 KK1016 pKa = 11.06 PVQRR1020 pKa = 11.84 GLLVHH1025 pKa = 5.96 YY1026 pKa = 8.04 PIIVLLQHH1034 pKa = 6.69 SLDD1037 pKa = 3.71 NNQIGEE1043 pKa = 4.34 LLRR1046 pKa = 11.84 AAGHH1050 pKa = 5.93 LCEE1053 pKa = 5.36 PGAEE1057 pKa = 3.86 RR1058 pKa = 11.84 IAAFGDD1064 pKa = 3.65 VKK1066 pKa = 10.74 LGSVINGWLPYY1077 pKa = 10.36 SDD1079 pKa = 4.55 AAALGARR1086 pKa = 11.84 GLWQTVRR1093 pKa = 11.84 VLYY1096 pKa = 8.93 PLRR1099 pKa = 11.84 MM1100 pKa = 3.89
Molecular weight: 118.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.671
IPC2_protein 8.507
IPC_protein 8.419
Toseland 8.595
ProMoST 8.902
Dawson 9.18
Bjellqvist 9.282
Wikipedia 9.311
Rodwell 9.238
Grimsley 8.975
Solomon 9.238
Lehninger 9.194
Nozaki 9.414
DTASelect 9.107
Thurlkill 9.151
EMBOSS 9.311
Sillero 9.385
Patrickios 4.685
IPC_peptide 9.224
IPC2_peptide 8.419
IPC2.peptide.svr19 7.887
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1899
799
1100
949.5
102.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.69 ± 0.111
2.001 ± 0.069
4.792 ± 0.05
5.319 ± 0.105
2.949 ± 0.59
10.321 ± 0.103
2.791 ± 0.259
3.476 ± 0.054
2.58 ± 0.043
10.216 ± 1.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.001 ± 0.069
1.896 ± 0.08
5.74 ± 0.988
2.528 ± 0.196
8.636 ± 0.769
7.162 ± 0.714
4.739 ± 1.058
6.635 ± 0.56
1.843 ± 0.159
2.686 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here