Mycobacterium phage Typha

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482JAP3|A0A482JAP3_9CAUD Uncharacterized protein OS=Mycobacterium phage Typha OX=2517971 GN=109 PE=4 SV=1
MM1 pKa = 7.97ADD3 pKa = 3.45FTVTGDD9 pKa = 3.15WTSILPGATLAADD22 pKa = 4.35LRR24 pKa = 11.84FQQMGNSADD33 pKa = 3.63VPGDD37 pKa = 3.24VHH39 pKa = 8.68ASIIDD44 pKa = 3.77GVLCDD49 pKa = 3.51ATGAEE54 pKa = 4.74DD55 pKa = 3.99VALTAPGAGATLFYY69 pKa = 10.05WVSINNLTVNGNAVTPRR86 pKa = 11.84RR87 pKa = 11.84FLIQAASANGEE98 pKa = 4.11VSLSALAPRR107 pKa = 11.84LFSMVEE113 pKa = 3.94GSTEE117 pKa = 3.93SGDD120 pKa = 3.85YY121 pKa = 9.83ATTDD125 pKa = 3.6YY126 pKa = 11.58VDD128 pKa = 3.36TAIANSGHH136 pKa = 6.51ATTADD141 pKa = 2.91IAAAVANLVSDD152 pKa = 4.18TDD154 pKa = 3.76LTAAIANFVTTSAMTTAIGNAVADD178 pKa = 4.12LVSSTEE184 pKa = 3.83LATALGDD191 pKa = 3.78YY192 pKa = 8.34VTADD196 pKa = 4.34DD197 pKa = 4.5LTSALSNRR205 pKa = 11.84PTTSAMNTAISNATSGLATTSDD227 pKa = 3.3VSNAVAGLASEE238 pKa = 4.74TYY240 pKa = 9.96VDD242 pKa = 3.71NAVLVPIVTGEE253 pKa = 4.1PTDD256 pKa = 3.87AAPDD260 pKa = 3.5GSMAIDD266 pKa = 3.4STGPTLYY273 pKa = 10.03MRR275 pKa = 11.84VEE277 pKa = 4.42GAWVAVTTAAA287 pKa = 4.52

Molecular weight: 28.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J6W6|A0A482J6W6_9CAUD Uncharacterized protein OS=Mycobacterium phage Typha OX=2517971 GN=102 PE=4 SV=1
MM1 pKa = 6.62TAKK4 pKa = 10.05GQHH7 pKa = 5.7RR8 pKa = 11.84SRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84GWRR15 pKa = 11.84YY16 pKa = 9.78RR17 pKa = 11.84GVHH20 pKa = 4.38HH21 pKa = 6.38TRR23 pKa = 11.84RR24 pKa = 11.84GALTVADD31 pKa = 4.45IKK33 pKa = 11.38NSLRR37 pKa = 11.84PNDD40 pKa = 3.6GQMIHH45 pKa = 6.81LGSVIKK51 pKa = 10.83NVTFFGLPPDD61 pKa = 4.79ADD63 pKa = 3.48PSMVEE68 pKa = 3.71ATLTLVGDD76 pKa = 4.15EE77 pKa = 4.22PTLTIAALTGPPGPGAGSS95 pKa = 3.17

Molecular weight: 10.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

23537

38

1527

185.3

20.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.008 ± 0.333

1.177 ± 0.136

6.913 ± 0.214

5.782 ± 0.214

2.396 ± 0.117

8.701 ± 0.438

2.545 ± 0.176

3.811 ± 0.165

3.301 ± 0.186

7.758 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.13

2.966 ± 0.154

5.961 ± 0.288

3.862 ± 0.151

6.891 ± 0.272

4.593 ± 0.181

7.053 ± 0.224

8.026 ± 0.22

2.226 ± 0.141

2.49 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski