Salmonella phage vB_SenTO17

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Cornellvirus; unclassified Cornellvirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3T8M8|A0A7G3T8M8_9CAUD Putative replicative helicase/primease OS=Salmonella phage vB_SenTO17 OX=2732254 GN=vBSenTO17_34 PE=4 SV=1
MM1 pKa = 6.5VARR4 pKa = 11.84YY5 pKa = 9.06RR6 pKa = 11.84FNYY9 pKa = 7.46VQEE12 pKa = 4.15MFEE15 pKa = 4.17TDD17 pKa = 3.69AGEE20 pKa = 4.25QYY22 pKa = 11.04CEE24 pKa = 4.14TEE26 pKa = 3.48RR27 pKa = 11.84DD28 pKa = 2.94EE29 pKa = 4.13WVRR32 pKa = 11.84YY33 pKa = 8.68EE34 pKa = 5.5DD35 pKa = 3.76YY36 pKa = 11.67AEE38 pKa = 4.33LKK40 pKa = 10.48EE41 pKa = 4.67LLDD44 pKa = 3.9ALAYY48 pKa = 10.45SVLNYY53 pKa = 9.63PLSPDD58 pKa = 3.48TEE60 pKa = 4.14EE61 pKa = 3.98LAKK64 pKa = 10.76GVLGVV69 pKa = 3.67

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3T2C3|A0A7G3T2C3_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenTO17 OX=2732254 GN=vBSenTO17_61 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84LTPKK6 pKa = 10.29EE7 pKa = 3.79PEE9 pKa = 3.64PRR11 pKa = 11.84KK12 pKa = 10.15RR13 pKa = 11.84CIQCRR18 pKa = 11.84EE19 pKa = 3.99RR20 pKa = 11.84LPLSSFHH27 pKa = 6.68KK28 pKa = 10.61SSVASIDD35 pKa = 3.61GYY37 pKa = 11.66RR38 pKa = 11.84NICKK42 pKa = 9.56SCRR45 pKa = 11.84RR46 pKa = 11.84ATEE49 pKa = 3.53AARR52 pKa = 11.84IRR54 pKa = 11.84EE55 pKa = 4.17KK56 pKa = 10.84RR57 pKa = 11.84NKK59 pKa = 9.7

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

13295

29

828

177.3

19.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.981 ± 0.519

1.249 ± 0.163

6.205 ± 0.24

6.281 ± 0.343

3.467 ± 0.177

7.665 ± 0.316

1.775 ± 0.188

5.265 ± 0.177

6.062 ± 0.359

7.522 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.127

4.769 ± 0.299

3.896 ± 0.217

4.024 ± 0.408

5.581 ± 0.224

5.874 ± 0.27

6.025 ± 0.329

6.92 ± 0.349

1.459 ± 0.16

3.452 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski