Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; Parabacteroides distasonis

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3830 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6LAJ8|A6LAJ8_PARD8 Putative integration host factor IHF alpha subunit OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=BDI_0944 PE=3 SV=1
MM1 pKa = 7.14LTKK4 pKa = 10.92SMISDD9 pKa = 4.2GLLQYY14 pKa = 10.42YY15 pKa = 9.51NWQTDD20 pKa = 3.51YY21 pKa = 11.77CLFTNTDD28 pKa = 2.98SMDD31 pKa = 3.86DD32 pKa = 3.62FLEE35 pKa = 4.48NEE37 pKa = 4.49LPDD40 pKa = 4.26DD41 pKa = 4.03YY42 pKa = 11.67EE43 pKa = 4.5VIEE46 pKa = 4.54RR47 pKa = 11.84DD48 pKa = 3.44RR49 pKa = 11.84NQCIVDD55 pKa = 3.64MDD57 pKa = 3.9GDD59 pKa = 3.82KK60 pKa = 11.67YY61 pKa = 11.16EE62 pKa = 3.87ITAYY66 pKa = 10.69GDD68 pKa = 3.47GDD70 pKa = 4.09FSHH73 pKa = 7.04HH74 pKa = 6.07VASIYY79 pKa = 10.6KK80 pKa = 10.58LSS82 pKa = 3.36

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A6L8D1|NADD_PARD8 Probable nicotinate-nucleotide adenylyltransferase OS=Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152) OX=435591 GN=nadD PE=3 SV=1
MM1 pKa = 7.63ILLKK5 pKa = 10.79SSFLFLVYY13 pKa = 10.14RR14 pKa = 11.84HH15 pKa = 5.87GNRR18 pKa = 11.84DD19 pKa = 3.06EE20 pKa = 4.31MISYY24 pKa = 9.55FLHH27 pKa = 5.94HH28 pKa = 6.71HH29 pKa = 5.88VPSKK33 pKa = 10.44KK34 pKa = 10.65SNIHH38 pKa = 4.88SCRR41 pKa = 11.84DD42 pKa = 3.29GACPVSSPPNGNLKK56 pKa = 9.99ILSDD60 pKa = 3.59SGRR63 pKa = 11.84RR64 pKa = 11.84GTPRR68 pKa = 11.84LYY70 pKa = 10.96NRR72 pKa = 11.84VITVLAPFRR81 pKa = 11.84LLPKK85 pKa = 10.43

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3830

0

3830

1437791

42

2472

375.4

42.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.951 ± 0.031

1.179 ± 0.015

5.56 ± 0.028

6.502 ± 0.031

4.673 ± 0.027

6.862 ± 0.033

1.805 ± 0.015

6.982 ± 0.034

6.447 ± 0.033

9.344 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.017

4.969 ± 0.033

3.91 ± 0.02

3.297 ± 0.021

4.765 ± 0.026

6.305 ± 0.03

5.506 ± 0.028

6.514 ± 0.03

1.241 ± 0.016

4.451 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski