Burkholderia phage ST79

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Nampongvirus; Burkholderia virus ST79

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JDL9|R4JDL9_9CAUD Protein LysC OS=Burkholderia phage ST79 OX=1282994 GN=ST79_028 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.32VRR4 pKa = 11.84AQQNEE9 pKa = 4.53TVDD12 pKa = 3.67ALCWRR17 pKa = 11.84WYY19 pKa = 10.37GRR21 pKa = 11.84TDD23 pKa = 3.46GVVEE27 pKa = 4.51AVLEE31 pKa = 4.35ANPGLADD38 pKa = 3.4IGLFLPLGFEE48 pKa = 4.63VEE50 pKa = 4.5MPDD53 pKa = 3.29PTGIAGTAPLVQLFDD68 pKa = 3.62

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JEU7|R4JEU7_9CAUD Helix-turn-helix domain protein OS=Burkholderia phage ST79 OX=1282994 GN=ST79_050 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84RR3 pKa = 11.84LLGTIGFAIAGLVSVIAWSAVDD25 pKa = 4.31SRR27 pKa = 11.84LCTVIKK33 pKa = 10.28NWCTPPAGTCGGGVDD48 pKa = 3.54ACAATTHH55 pKa = 5.57ATMDD59 pKa = 3.59LFVYY63 pKa = 10.32LFGPPILFAALGFFVFTRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84PSRR86 pKa = 11.84IVVAYY91 pKa = 10.05LVGAVIAQWVLWFIGVRR108 pKa = 11.84LLHH111 pKa = 6.39II112 pKa = 4.66

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11120

48

1121

226.9

24.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.725 ± 0.436

0.908 ± 0.158

6.583 ± 0.31

4.928 ± 0.327

3.399 ± 0.18

8.471 ± 0.595

1.781 ± 0.176

4.64 ± 0.192

3.723 ± 0.31

7.698 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.138

3.399 ± 0.526

4.64 ± 0.371

3.264 ± 0.211

8.04 ± 0.474

5.441 ± 0.256

6.106 ± 0.281

7.599 ± 0.293

1.799 ± 0.149

2.428 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski