Amino acid dipepetide frequency for Escherichia Stx1-converting recombinant phage HUN/2013

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.289AlaAla: 8.289 ± 0.761
1.184AlaCys: 1.184 ± 0.353
5.075AlaAsp: 5.075 ± 0.603
8.12AlaGlu: 8.12 ± 0.739
3.496AlaPhe: 3.496 ± 0.544
7.443AlaGly: 7.443 ± 1.071
1.41AlaHis: 1.41 ± 0.342
4.793AlaIle: 4.793 ± 0.56
4.511AlaLys: 4.511 ± 0.465
7.105AlaLeu: 7.105 ± 0.656
3.045AlaMet: 3.045 ± 0.518
2.876AlaAsn: 2.876 ± 0.34
3.045AlaPro: 3.045 ± 0.458
5.357AlaGln: 5.357 ± 0.722
5.469AlaArg: 5.469 ± 0.612
5.639AlaSer: 5.639 ± 0.519
5.413AlaThr: 5.413 ± 0.547
6.202AlaVal: 6.202 ± 0.593
1.917AlaTrp: 1.917 ± 0.345
2.537AlaTyr: 2.537 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
1.184CysAla: 1.184 ± 0.32
0.338CysCys: 0.338 ± 0.174
0.62CysAsp: 0.62 ± 0.208
0.789CysGlu: 0.789 ± 0.244
0.395CysPhe: 0.395 ± 0.154
0.959CysGly: 0.959 ± 0.318
0.564CysHis: 0.564 ± 0.199
0.564CysIle: 0.564 ± 0.218
0.507CysLys: 0.507 ± 0.206
0.902CysLeu: 0.902 ± 0.279
0.056CysMet: 0.056 ± 0.056
0.282CysAsn: 0.282 ± 0.136
0.62CysPro: 0.62 ± 0.185
0.395CysGln: 0.395 ± 0.16
1.071CysArg: 1.071 ± 0.315
1.128CysSer: 1.128 ± 0.317
0.507CysThr: 0.507 ± 0.199
0.846CysVal: 0.846 ± 0.242
0.226CysTrp: 0.226 ± 0.116
0.282CysTyr: 0.282 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
6.09AspAla: 6.09 ± 0.665
0.789AspCys: 0.789 ± 0.278
4.173AspAsp: 4.173 ± 0.567
4.398AspGlu: 4.398 ± 0.578
1.748AspPhe: 1.748 ± 0.329
4.849AspGly: 4.849 ± 0.567
0.789AspHis: 0.789 ± 0.301
3.44AspIle: 3.44 ± 0.381
4.229AspLys: 4.229 ± 0.435
4.285AspLeu: 4.285 ± 0.464
1.804AspMet: 1.804 ± 0.279
2.819AspAsn: 2.819 ± 0.411
2.65AspPro: 2.65 ± 0.382
1.579AspGln: 1.579 ± 0.364
3.214AspArg: 3.214 ± 0.448
3.552AspSer: 3.552 ± 0.446
2.707AspThr: 2.707 ± 0.422
4.06AspVal: 4.06 ± 0.387
1.128AspTrp: 1.128 ± 0.392
1.804AspTyr: 1.804 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
6.428GluAla: 6.428 ± 0.603
1.184GluCys: 1.184 ± 0.296
2.143GluAsp: 2.143 ± 0.338
4.06GluGlu: 4.06 ± 0.455
2.368GluPhe: 2.368 ± 0.4
3.834GluGly: 3.834 ± 0.541
1.015GluHis: 1.015 ± 0.229
3.947GluIle: 3.947 ± 0.502
5.018GluLys: 5.018 ± 0.651
5.864GluLeu: 5.864 ± 0.666
2.594GluMet: 2.594 ± 0.346
3.045GluAsn: 3.045 ± 0.555
1.917GluPro: 1.917 ± 0.48
4.511GluGln: 4.511 ± 0.578
5.639GluArg: 5.639 ± 0.619
3.327GluSer: 3.327 ± 0.442
3.778GluThr: 3.778 ± 0.648
4.003GluVal: 4.003 ± 0.561
0.902GluTrp: 0.902 ± 0.21
1.973GluTyr: 1.973 ± 0.342
0.0GluXaa: 0.0 ± 0.0
Phe
2.763PheAla: 2.763 ± 0.35
0.507PheCys: 0.507 ± 0.184
1.748PheAsp: 1.748 ± 0.345
1.41PheGlu: 1.41 ± 0.357
1.015PhePhe: 1.015 ± 0.286
2.255PheGly: 2.255 ± 0.276
0.677PheHis: 0.677 ± 0.174
1.748PheIle: 1.748 ± 0.277
1.748PheLys: 1.748 ± 0.346
1.973PheLeu: 1.973 ± 0.365
1.128PheMet: 1.128 ± 0.252
1.41PheAsn: 1.41 ± 0.295
1.297PhePro: 1.297 ± 0.326
1.015PheGln: 1.015 ± 0.245
2.199PheArg: 2.199 ± 0.341
2.988PheSer: 2.988 ± 0.439
2.537PheThr: 2.537 ± 0.348
2.368PheVal: 2.368 ± 0.336
0.846PheTrp: 0.846 ± 0.211
1.015PheTyr: 1.015 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
6.202GlyAla: 6.202 ± 0.957
0.902GlyCys: 0.902 ± 0.276
4.962GlyAsp: 4.962 ± 0.792
6.033GlyGlu: 6.033 ± 1.163
2.707GlyPhe: 2.707 ± 0.45
5.413GlyGly: 5.413 ± 0.665
1.071GlyHis: 1.071 ± 0.259
4.173GlyIle: 4.173 ± 0.601
4.624GlyLys: 4.624 ± 0.914
4.68GlyLeu: 4.68 ± 0.467
2.65GlyMet: 2.65 ± 0.477
2.819GlyAsn: 2.819 ± 0.379
3.721GlyPro: 3.721 ± 1.963
2.819GlyGln: 2.819 ± 0.374
4.285GlyArg: 4.285 ± 0.555
3.891GlySer: 3.891 ± 0.448
3.721GlyThr: 3.721 ± 0.616
5.413GlyVal: 5.413 ± 0.533
1.071GlyTrp: 1.071 ± 0.253
2.594GlyTyr: 2.594 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
1.466HisAla: 1.466 ± 0.252
0.169HisCys: 0.169 ± 0.106
0.959HisAsp: 0.959 ± 0.198
1.015HisGlu: 1.015 ± 0.236
0.733HisPhe: 0.733 ± 0.254
1.861HisGly: 1.861 ± 0.41
0.451HisHis: 0.451 ± 0.137
0.507HisIle: 0.507 ± 0.168
1.128HisLys: 1.128 ± 0.299
1.579HisLeu: 1.579 ± 0.451
0.226HisMet: 0.226 ± 0.115
1.015HisAsn: 1.015 ± 0.206
0.846HisPro: 0.846 ± 0.221
0.564HisGln: 0.564 ± 0.219
0.846HisArg: 0.846 ± 0.25
0.846HisSer: 0.846 ± 0.187
1.184HisThr: 1.184 ± 0.29
0.959HisVal: 0.959 ± 0.193
0.451HisTrp: 0.451 ± 0.206
0.507HisTyr: 0.507 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.962IleAla: 4.962 ± 0.514
1.128IleCys: 1.128 ± 0.28
4.398IleAsp: 4.398 ± 0.612
3.101IleGlu: 3.101 ± 0.53
1.128IlePhe: 1.128 ± 0.281
3.214IleGly: 3.214 ± 0.481
0.789IleHis: 0.789 ± 0.188
2.255IleIle: 2.255 ± 0.355
3.045IleLys: 3.045 ± 0.416
3.496IleLeu: 3.496 ± 0.649
1.24IleMet: 1.24 ± 0.247
2.988IleAsn: 2.988 ± 0.352
2.425IlePro: 2.425 ± 0.335
1.748IleGln: 1.748 ± 0.265
4.511IleArg: 4.511 ± 0.387
3.778IleSer: 3.778 ± 0.584
4.173IleThr: 4.173 ± 0.6
2.143IleVal: 2.143 ± 0.42
0.395IleTrp: 0.395 ± 0.162
1.635IleTyr: 1.635 ± 0.369
0.0IleXaa: 0.0 ± 0.0
Lys
5.977LysAla: 5.977 ± 0.52
0.338LysCys: 0.338 ± 0.199
3.383LysAsp: 3.383 ± 0.493
3.609LysGlu: 3.609 ± 0.482
0.959LysPhe: 0.959 ± 0.206
5.582LysGly: 5.582 ± 1.115
0.902LysHis: 0.902 ± 0.241
3.552LysIle: 3.552 ± 0.458
3.947LysLys: 3.947 ± 0.596
5.075LysLeu: 5.075 ± 0.567
1.861LysMet: 1.861 ± 0.317
3.665LysAsn: 3.665 ± 0.491
2.537LysPro: 2.537 ± 0.337
3.158LysGln: 3.158 ± 0.548
2.876LysArg: 2.876 ± 0.378
2.763LysSer: 2.763 ± 0.413
3.496LysThr: 3.496 ± 0.526
3.27LysVal: 3.27 ± 0.492
0.789LysTrp: 0.789 ± 0.244
1.861LysTyr: 1.861 ± 0.384
0.0LysXaa: 0.0 ± 0.0
Leu
7.95LeuAla: 7.95 ± 0.858
1.24LeuCys: 1.24 ± 0.345
4.003LeuAsp: 4.003 ± 0.591
4.116LeuGlu: 4.116 ± 0.652
2.876LeuPhe: 2.876 ± 0.486
4.229LeuGly: 4.229 ± 0.479
1.353LeuHis: 1.353 ± 0.266
4.173LeuIle: 4.173 ± 0.557
4.567LeuLys: 4.567 ± 0.513
6.146LeuLeu: 6.146 ± 0.557
2.255LeuMet: 2.255 ± 0.354
4.229LeuAsn: 4.229 ± 0.536
3.721LeuPro: 3.721 ± 0.457
3.27LeuGln: 3.27 ± 0.697
5.526LeuArg: 5.526 ± 0.542
4.962LeuSer: 4.962 ± 0.483
4.793LeuThr: 4.793 ± 0.506
4.624LeuVal: 4.624 ± 0.378
0.959LeuTrp: 0.959 ± 0.248
2.312LeuTyr: 2.312 ± 0.305
0.0LeuXaa: 0.0 ± 0.0
Met
3.27MetAla: 3.27 ± 0.356
0.056MetCys: 0.056 ± 0.048
1.41MetAsp: 1.41 ± 0.274
1.692MetGlu: 1.692 ± 0.286
0.846MetPhe: 0.846 ± 0.196
1.635MetGly: 1.635 ± 0.37
0.395MetHis: 0.395 ± 0.121
1.071MetIle: 1.071 ± 0.222
2.199MetLys: 2.199 ± 0.386
2.199MetLeu: 2.199 ± 0.304
0.846MetMet: 0.846 ± 0.219
1.579MetAsn: 1.579 ± 0.316
1.692MetPro: 1.692 ± 0.303
1.579MetGln: 1.579 ± 0.295
1.579MetArg: 1.579 ± 0.228
2.255MetSer: 2.255 ± 0.381
2.594MetThr: 2.594 ± 0.321
1.353MetVal: 1.353 ± 0.293
0.226MetTrp: 0.226 ± 0.113
0.451MetTyr: 0.451 ± 0.191
0.0MetXaa: 0.0 ± 0.0
Asn
4.68AsnAla: 4.68 ± 0.743
0.564AsnCys: 0.564 ± 0.202
2.481AsnAsp: 2.481 ± 0.34
2.819AsnGlu: 2.819 ± 0.347
1.128AsnPhe: 1.128 ± 0.214
3.158AsnGly: 3.158 ± 0.475
1.466AsnHis: 1.466 ± 0.339
2.594AsnIle: 2.594 ± 0.423
2.368AsnLys: 2.368 ± 0.353
3.496AsnLeu: 3.496 ± 0.415
1.015AsnMet: 1.015 ± 0.237
1.804AsnAsn: 1.804 ± 0.375
1.804AsnPro: 1.804 ± 0.31
2.199AsnGln: 2.199 ± 0.298
3.101AsnArg: 3.101 ± 0.506
2.65AsnSer: 2.65 ± 0.347
2.255AsnThr: 2.255 ± 0.35
2.143AsnVal: 2.143 ± 0.402
0.395AsnTrp: 0.395 ± 0.132
1.24AsnTyr: 1.24 ± 0.299
0.0AsnXaa: 0.0 ± 0.0
Pro
3.609ProAla: 3.609 ± 0.631
0.395ProCys: 0.395 ± 0.154
4.342ProAsp: 4.342 ± 0.521
4.962ProGlu: 4.962 ± 0.67
1.41ProPhe: 1.41 ± 0.277
3.27ProGly: 3.27 ± 0.569
0.564ProHis: 0.564 ± 0.204
1.128ProIle: 1.128 ± 0.268
2.876ProLys: 2.876 ± 0.677
2.65ProLeu: 2.65 ± 0.356
0.902ProMet: 0.902 ± 0.185
0.789ProAsn: 0.789 ± 0.183
1.41ProPro: 1.41 ± 0.287
2.425ProGln: 2.425 ± 0.475
1.804ProArg: 1.804 ± 0.357
2.594ProSer: 2.594 ± 0.4
1.804ProThr: 1.804 ± 0.312
4.173ProVal: 4.173 ± 0.536
0.62ProTrp: 0.62 ± 0.207
1.522ProTyr: 1.522 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
4.906GlnAla: 4.906 ± 0.564
0.733GlnCys: 0.733 ± 0.203
2.537GlnAsp: 2.537 ± 0.334
3.158GlnGlu: 3.158 ± 0.493
1.353GlnPhe: 1.353 ± 0.366
3.045GlnGly: 3.045 ± 0.706
0.733GlnHis: 0.733 ± 0.181
2.65GlnIle: 2.65 ± 0.518
3.496GlnLys: 3.496 ± 0.519
3.383GlnLeu: 3.383 ± 0.382
1.24GlnMet: 1.24 ± 0.261
1.579GlnAsn: 1.579 ± 0.35
2.03GlnPro: 2.03 ± 0.414
4.003GlnGln: 4.003 ± 0.863
3.44GlnArg: 3.44 ± 0.651
2.763GlnSer: 2.763 ± 0.336
2.086GlnThr: 2.086 ± 0.379
2.368GlnVal: 2.368 ± 0.489
0.677GlnTrp: 0.677 ± 0.203
1.353GlnTyr: 1.353 ± 0.291
0.0GlnXaa: 0.0 ± 0.0
Arg
4.511ArgAla: 4.511 ± 0.458
0.226ArgCys: 0.226 ± 0.12
4.285ArgAsp: 4.285 ± 0.59
5.187ArgGlu: 5.187 ± 0.7
2.312ArgPhe: 2.312 ± 0.363
4.906ArgGly: 4.906 ± 0.947
1.692ArgHis: 1.692 ± 0.252
3.552ArgIle: 3.552 ± 0.524
4.342ArgLys: 4.342 ± 0.614
5.131ArgLeu: 5.131 ± 0.502
2.199ArgMet: 2.199 ± 0.341
3.27ArgAsn: 3.27 ± 0.4
2.086ArgPro: 2.086 ± 0.394
2.988ArgGln: 2.988 ± 0.412
5.3ArgArg: 5.3 ± 0.565
3.609ArgSer: 3.609 ± 0.404
3.891ArgThr: 3.891 ± 0.573
3.778ArgVal: 3.778 ± 0.518
1.184ArgTrp: 1.184 ± 0.222
2.255ArgTyr: 2.255 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
5.526SerAla: 5.526 ± 0.456
0.733SerCys: 0.733 ± 0.219
3.665SerAsp: 3.665 ± 0.481
3.947SerGlu: 3.947 ± 0.529
1.748SerPhe: 1.748 ± 0.285
5.639SerGly: 5.639 ± 0.652
0.959SerHis: 0.959 ± 0.233
2.312SerIle: 2.312 ± 0.353
2.65SerLys: 2.65 ± 0.479
5.92SerLeu: 5.92 ± 0.638
1.41SerMet: 1.41 ± 0.25
2.537SerAsn: 2.537 ± 0.451
3.045SerPro: 3.045 ± 0.397
3.158SerGln: 3.158 ± 0.438
4.229SerArg: 4.229 ± 0.637
3.101SerSer: 3.101 ± 0.636
2.932SerThr: 2.932 ± 0.445
3.834SerVal: 3.834 ± 0.451
0.846SerTrp: 0.846 ± 0.223
1.804SerTyr: 1.804 ± 0.333
0.0SerXaa: 0.0 ± 0.0
Thr
5.357ThrAla: 5.357 ± 0.471
0.451ThrCys: 0.451 ± 0.179
3.44ThrAsp: 3.44 ± 0.399
3.44ThrGlu: 3.44 ± 0.441
2.255ThrPhe: 2.255 ± 0.489
6.09ThrGly: 6.09 ± 0.851
0.959ThrHis: 0.959 ± 0.246
3.665ThrIle: 3.665 ± 0.457
2.876ThrLys: 2.876 ± 0.401
5.187ThrLeu: 5.187 ± 0.478
0.846ThrMet: 0.846 ± 0.207
1.692ThrAsn: 1.692 ± 0.237
3.609ThrPro: 3.609 ± 0.382
1.917ThrGln: 1.917 ± 0.387
2.65ThrArg: 2.65 ± 0.365
3.327ThrSer: 3.327 ± 0.516
3.327ThrThr: 3.327 ± 0.475
4.003ThrVal: 4.003 ± 0.634
0.846ThrTrp: 0.846 ± 0.208
1.184ThrTyr: 1.184 ± 0.309
0.0ThrXaa: 0.0 ± 0.0
Val
5.92ValAla: 5.92 ± 0.535
0.846ValCys: 0.846 ± 0.281
3.891ValAsp: 3.891 ± 0.471
3.552ValGlu: 3.552 ± 0.367
2.199ValPhe: 2.199 ± 0.298
3.44ValGly: 3.44 ± 0.453
0.677ValHis: 0.677 ± 0.182
3.947ValIle: 3.947 ± 0.513
3.383ValLys: 3.383 ± 0.456
5.3ValLeu: 5.3 ± 0.61
1.804ValMet: 1.804 ± 0.293
3.045ValAsn: 3.045 ± 0.325
2.876ValPro: 2.876 ± 0.375
2.312ValGln: 2.312 ± 0.307
5.187ValArg: 5.187 ± 1.054
4.173ValSer: 4.173 ± 0.518
3.778ValThr: 3.778 ± 0.511
4.454ValVal: 4.454 ± 0.528
0.733ValTrp: 0.733 ± 0.189
1.41ValTyr: 1.41 ± 0.235
0.0ValXaa: 0.0 ± 0.0
Trp
0.959TrpAla: 0.959 ± 0.29
0.113TrpCys: 0.113 ± 0.093
0.789TrpAsp: 0.789 ± 0.219
0.564TrpGlu: 0.564 ± 0.175
0.677TrpPhe: 0.677 ± 0.161
0.62TrpGly: 0.62 ± 0.167
0.338TrpHis: 0.338 ± 0.138
0.789TrpIle: 0.789 ± 0.208
1.071TrpLys: 1.071 ± 0.229
1.466TrpLeu: 1.466 ± 0.329
0.902TrpMet: 0.902 ± 0.227
0.677TrpAsn: 0.677 ± 0.222
0.677TrpPro: 0.677 ± 0.209
1.071TrpGln: 1.071 ± 0.215
1.24TrpArg: 1.24 ± 0.286
0.902TrpSer: 0.902 ± 0.258
0.507TrpThr: 0.507 ± 0.172
1.071TrpVal: 1.071 ± 0.262
0.338TrpTrp: 0.338 ± 0.161
0.395TrpTyr: 0.395 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.876TyrAla: 2.876 ± 0.424
0.338TyrCys: 0.338 ± 0.141
1.804TyrAsp: 1.804 ± 0.323
1.579TyrGlu: 1.579 ± 0.358
1.297TyrPhe: 1.297 ± 0.243
2.312TyrGly: 2.312 ± 0.368
0.507TyrHis: 0.507 ± 0.19
1.692TyrIle: 1.692 ± 0.35
1.015TyrLys: 1.015 ± 0.23
1.522TyrLeu: 1.522 ± 0.337
0.846TyrMet: 0.846 ± 0.228
1.24TyrAsn: 1.24 ± 0.294
1.24TyrPro: 1.24 ± 0.253
1.466TyrGln: 1.466 ± 0.271
2.537TyrArg: 2.537 ± 0.473
1.748TyrSer: 1.748 ± 0.32
1.635TyrThr: 1.635 ± 0.434
1.861TyrVal: 1.861 ± 0.29
0.62TyrTrp: 0.62 ± 0.156
0.902TyrTyr: 0.902 ± 0.189
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (17736 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski