Corynebacterium camporealensis
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2245 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6T9I7|A0A0F6T9I7_9CORY Penicillin-insensitive transglycosylase OS=Corynebacterium camporealensis OX=161896 GN=NG00_00154 PE=4 SV=1
MM1 pKa = 7.96 PSGTLTKK8 pKa = 11.0 YY9 pKa = 10.13 NVQDD13 pKa = 3.98 EE14 pKa = 4.41 LHH16 pKa = 6.52 EE17 pKa = 4.62 ALDD20 pKa = 3.8 AAGAEE25 pKa = 4.14 ITVAIQDD32 pKa = 4.11 GATLTAGTHH41 pKa = 5.29 YY42 pKa = 10.39 NVSITGQNILVEE54 pKa = 4.48 FTQAGFDD61 pKa = 3.87 AIEE64 pKa = 4.58 AGDD67 pKa = 3.96 LVEE70 pKa = 4.32 TTITTRR76 pKa = 11.84 TTEE79 pKa = 4.1 AVQHH83 pKa = 6.22 IPNDD87 pKa = 3.07 ATLIVNNGSGDD98 pKa = 3.83 TEE100 pKa = 4.33 TTSDD104 pKa = 4.76 DD105 pKa = 3.28 VHH107 pKa = 6.52 TYY109 pKa = 8.7 WGNLNINKK117 pKa = 9.21 IDD119 pKa = 4.1 GDD121 pKa = 4.08 SEE123 pKa = 4.52 APLEE127 pKa = 4.35 GAVFQLLQCQAEE139 pKa = 4.28 GDD141 pKa = 3.6 GWVQVDD147 pKa = 3.63 GTEE150 pKa = 4.03 PLNVNGQTEE159 pKa = 4.52 WTTDD163 pKa = 2.83 ADD165 pKa = 4.02 GAVTISGIHH174 pKa = 4.39 VTDD177 pKa = 3.74 YY178 pKa = 11.77 ANNADD183 pKa = 4.44 DD184 pKa = 4.04 TTVDD188 pKa = 3.54 YY189 pKa = 11.34 CLLEE193 pKa = 3.98 TDD195 pKa = 4.73 APDD198 pKa = 4.33 GYY200 pKa = 10.98 VGNDD204 pKa = 3.15 ALVHH208 pKa = 5.82 FEE210 pKa = 4.48 LNRR213 pKa = 11.84 GDD215 pKa = 3.8 VDD217 pKa = 4.1 PEE219 pKa = 4.18 TGAPTAQIQYY229 pKa = 8.31 TATIEE234 pKa = 4.13 NFTDD238 pKa = 3.76 EE239 pKa = 4.45 NRR241 pKa = 11.84 LPNTGGMGVGLLIALGALIIGGGAYY266 pKa = 9.99 AARR269 pKa = 11.84 RR270 pKa = 11.84 NSAKK274 pKa = 10.61 AA275 pKa = 3.16
Molecular weight: 28.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.088
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.872
Patrickios 1.163
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A0F6TCB9|A0A0F6TCB9_9CORY Uncharacterized protein OS=Corynebacterium camporealensis OX=161896 GN=NG00_02025 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.59 HH19 pKa = 4.63 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MNTRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.72 KK40 pKa = 10.5 GRR42 pKa = 11.84 AKK44 pKa = 9.67 LTAA47 pKa = 4.21
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2245
0
2245
737566
40
3018
328.5
35.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.329 ± 0.065
0.706 ± 0.015
6.348 ± 0.047
6.817 ± 0.054
3.393 ± 0.037
8.216 ± 0.057
2.118 ± 0.026
5.141 ± 0.041
3.44 ± 0.041
9.57 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.254 ± 0.025
3.121 ± 0.031
5.002 ± 0.037
3.516 ± 0.029
5.918 ± 0.046
5.665 ± 0.034
5.831 ± 0.031
7.89 ± 0.042
1.391 ± 0.025
2.333 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here