Plasmodium falciparum RAJ116
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3177 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L0D0G9|A0A0L0D0G9_PLAFA Uncharacterized protein OS=Plasmodium falciparum RAJ116 OX=580058 GN=PFLG_02945 PE=4 SV=1
MM1 pKa = 7.56 SYY3 pKa = 10.52 VGPYY7 pKa = 9.72 CEE9 pKa = 4.43 CKK11 pKa = 10.55 CNIGHH16 pKa = 6.99 KK17 pKa = 10.41 VGSSWYY23 pKa = 10.54 NNDD26 pKa = 5.38 DD27 pKa = 3.36 INDD30 pKa = 3.94 VIKK33 pKa = 10.95 HH34 pKa = 5.36 EE35 pKa = 4.84 FGSSEE40 pKa = 4.11 YY41 pKa = 11.1 NNDD44 pKa = 4.63 DD45 pKa = 4.2 ISDD48 pKa = 4.11 DD49 pKa = 3.86 SSKK52 pKa = 10.8 KK53 pKa = 10.79 GLMIDD58 pKa = 4.26 MIMQVSATLDD68 pKa = 3.33 LDD70 pKa = 4.65 LYY72 pKa = 11.27 NNDD75 pKa = 5.37 DD76 pKa = 4.01 ISDD79 pKa = 4.13 DD80 pKa = 3.74 SSKK83 pKa = 11.27 KK84 pKa = 10.61 LVINDD89 pKa = 4.27 IIVHH93 pKa = 5.25 VSAPEE98 pKa = 3.86 EE99 pKa = 4.36 TEE101 pKa = 3.92 LL102 pKa = 5.34
Molecular weight: 11.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.999
IPC_protein 3.973
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.808
Grimsley 3.668
Solomon 3.973
Lehninger 3.923
Nozaki 4.101
DTASelect 4.355
Thurlkill 3.821
EMBOSS 3.935
Sillero 4.101
Patrickios 1.952
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.994
Protein with the highest isoelectric point:
>tr|A0A0L0CS98|A0A0L0CS98_PLAFA Uncharacterized protein OS=Plasmodium falciparum RAJ116 OX=580058 GN=PFLG_00113 PE=4 SV=1
MM1 pKa = 7.21 LVKK4 pKa = 10.29 VRR6 pKa = 11.84 PVLVKK11 pKa = 10.49 LRR13 pKa = 11.84 PILVNLRR20 pKa = 11.84 PKK22 pKa = 10.24 LVNLRR27 pKa = 11.84 PVLVKK32 pKa = 10.55 LRR34 pKa = 11.84 PTLVNLRR41 pKa = 11.84 PQLMKK46 pKa = 10.45 VRR48 pKa = 11.84 SMLVKK53 pKa = 10.36 LVKK56 pKa = 10.06 LRR58 pKa = 11.84 PMLVKK63 pKa = 10.45 LGPMLATLRR72 pKa = 11.84 PMLVKK77 pKa = 10.62 LRR79 pKa = 11.84 PKK81 pKa = 9.98 LVKK84 pKa = 10.28 LRR86 pKa = 11.84 PQLMNVRR93 pKa = 11.84 PMLVKK98 pKa = 10.33 VRR100 pKa = 11.84 PKK102 pKa = 10.27 LVKK105 pKa = 10.16 LRR107 pKa = 11.84 AMLVKK112 pKa = 10.11 LRR114 pKa = 11.84 PMLVEE119 pKa = 3.99 VV120 pKa = 4.22
Molecular weight: 13.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.096
IPC_protein 12.647
Toseland 12.822
ProMoST 13.32
Dawson 12.837
Bjellqvist 12.822
Wikipedia 13.305
Rodwell 12.647
Grimsley 12.866
Solomon 13.32
Lehninger 13.217
Nozaki 12.822
DTASelect 12.822
Thurlkill 12.822
EMBOSS 13.32
Sillero 12.822
Patrickios 12.354
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3177
0
3177
2165978
40
10229
681.8
80.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.954 ± 0.031
1.791 ± 0.024
6.703 ± 0.045
7.326 ± 0.089
4.07 ± 0.035
3.029 ± 0.038
2.446 ± 0.02
8.871 ± 0.055
11.608 ± 0.055
7.196 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.259 ± 0.02
14.623 ± 0.133
2.105 ± 0.027
2.772 ± 0.026
2.669 ± 0.022
6.507 ± 0.04
4.161 ± 0.033
3.877 ± 0.046
0.524 ± 0.013
5.509 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here