Mongoose feces-associated gemycircularvirus b

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus monas1; Mongoose associated gemykibivirus 1

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3M1X5|A0A0E3M1X5_9VIRU Rep OS=Mongoose feces-associated gemycircularvirus b OX=1634486 PE=3 SV=1
MM1 pKa = 6.85VQYY4 pKa = 10.78NVNNWTIRR12 pKa = 11.84PFRR15 pKa = 11.84KK16 pKa = 9.8LMSSFRR22 pKa = 11.84FQARR26 pKa = 11.84YY27 pKa = 10.11GLFTYY32 pKa = 7.67SQCAQLRR39 pKa = 11.84PDD41 pKa = 5.5AISQHH46 pKa = 5.64YY47 pKa = 8.63EE48 pKa = 3.68AFGADD53 pKa = 3.8YY54 pKa = 10.53IIGRR58 pKa = 11.84EE59 pKa = 3.94THH61 pKa = 6.42SDD63 pKa = 3.12GGTHH67 pKa = 4.98YY68 pKa = 10.73HH69 pKa = 5.75VFVDD73 pKa = 4.7FRR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.91YY78 pKa = 8.97RR79 pKa = 11.84TRR81 pKa = 11.84DD82 pKa = 3.11CRR84 pKa = 11.84KK85 pKa = 8.28FDD87 pKa = 3.69VEE89 pKa = 4.38GFHH92 pKa = 6.93PNIVPSLRR100 pKa = 11.84NPAGGWDD107 pKa = 4.0YY108 pKa = 11.02ATKK111 pKa = 10.74DD112 pKa = 3.63DD113 pKa = 5.46DD114 pKa = 3.76ICGGRR119 pKa = 11.84LRR121 pKa = 11.84RR122 pKa = 11.84PEE124 pKa = 4.06STEE127 pKa = 3.84GNQSGRR133 pKa = 11.84QNAGWDD139 pKa = 3.65EE140 pKa = 4.03LRR142 pKa = 11.84SAEE145 pKa = 4.15TRR147 pKa = 11.84DD148 pKa = 3.4DD149 pKa = 3.8FFRR152 pKa = 11.84IASEE156 pKa = 4.06HH157 pKa = 6.19LFSHH161 pKa = 6.51LVKK164 pKa = 10.53SFHH167 pKa = 6.75SFSAYY172 pKa = 10.39ADD174 pKa = 2.9WKK176 pKa = 9.89YY177 pKa = 10.85RR178 pKa = 11.84VDD180 pKa = 3.73RR181 pKa = 11.84SPYY184 pKa = 7.31QHH186 pKa = 6.44NPEE189 pKa = 4.01FHH191 pKa = 7.18FDD193 pKa = 3.56PGKK196 pKa = 10.77LGTLRR201 pKa = 11.84EE202 pKa = 3.77WHH204 pKa = 5.96GRR206 pKa = 11.84YY207 pKa = 9.75LSDD210 pKa = 4.41HH211 pKa = 6.32IVGKK215 pKa = 9.7GIRR218 pKa = 11.84GMSLLAWGRR227 pKa = 11.84SKK229 pKa = 9.78TGKK232 pKa = 8.59TMWARR237 pKa = 11.84SLGKK241 pKa = 9.22HH242 pKa = 5.95AYY244 pKa = 9.85FGGLFSLDD252 pKa = 2.85EE253 pKa = 4.24WEE255 pKa = 4.07QDD257 pKa = 3.12EE258 pKa = 4.69SVDD261 pKa = 3.64YY262 pKa = 11.3AVFDD266 pKa = 5.29DD267 pKa = 3.86IQGGFKK273 pKa = 10.47FFPAYY278 pKa = 9.75KK279 pKa = 9.8SWLGQQTEE287 pKa = 4.45FHH289 pKa = 6.2CTDD292 pKa = 3.53RR293 pKa = 11.84YY294 pKa = 10.06RR295 pKa = 11.84KK296 pKa = 9.4KK297 pKa = 10.77KK298 pKa = 8.75HH299 pKa = 4.86VQWGKK304 pKa = 8.63PCIWLMNEE312 pKa = 4.66DD313 pKa = 5.19PYY315 pKa = 11.33QQDD318 pKa = 3.06VDD320 pKa = 4.11IDD322 pKa = 3.65WLEE325 pKa = 4.12ANCLIVNVDD334 pKa = 3.02NSLFF338 pKa = 3.65

Molecular weight: 39.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3M1X5|A0A0E3M1X5_9VIRU Rep OS=Mongoose feces-associated gemycircularvirus b OX=1634486 PE=3 SV=1
MM1 pKa = 6.95THH3 pKa = 6.2SKK5 pKa = 8.6MAYY8 pKa = 7.83RR9 pKa = 11.84SRR11 pKa = 11.84RR12 pKa = 11.84FTGRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84GFTPRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84SSFRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84SFRR35 pKa = 11.84SRR37 pKa = 11.84PTRR40 pKa = 11.84NYY42 pKa = 9.69RR43 pKa = 11.84RR44 pKa = 11.84PRR46 pKa = 11.84TNVRR50 pKa = 11.84RR51 pKa = 11.84VRR53 pKa = 11.84NLASRR58 pKa = 11.84KK59 pKa = 9.83CKK61 pKa = 10.56DD62 pKa = 3.39SMLSTPIDD70 pKa = 3.5DD71 pKa = 3.98TGTPEE76 pKa = 4.05TPGPVSMNAANVYY89 pKa = 10.06GFIYY93 pKa = 10.36SPSARR98 pKa = 11.84VAGHH102 pKa = 6.96LLDD105 pKa = 3.83SGEE108 pKa = 4.25RR109 pKa = 11.84VNTPSHH115 pKa = 4.48SQRR118 pKa = 11.84RR119 pKa = 11.84KK120 pKa = 7.75SKK122 pKa = 10.46CYY124 pKa = 8.68VRR126 pKa = 11.84GYY128 pKa = 10.86GEE130 pKa = 4.14TVEE133 pKa = 4.56IQTDD137 pKa = 3.97DD138 pKa = 3.42ASPWIWRR145 pKa = 11.84RR146 pKa = 11.84IVFSTIGLAVQFSPGLLYY164 pKa = 11.29NNDD167 pKa = 3.29DD168 pKa = 3.31TRR170 pKa = 11.84GYY172 pKa = 10.07GRR174 pKa = 11.84TMYY177 pKa = 10.34NLRR180 pKa = 11.84SGTPQADD187 pKa = 3.56TLEE190 pKa = 5.0GIVYY194 pKa = 9.99RR195 pKa = 11.84YY196 pKa = 9.79LFQGTQDD203 pKa = 3.39IDD205 pKa = 3.25WANVMTATTAPRR217 pKa = 11.84HH218 pKa = 5.06VKK220 pKa = 10.05VYY222 pKa = 9.86SDD224 pKa = 3.52RR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 11.84IIQSPNSDD235 pKa = 2.92GGTIRR240 pKa = 11.84ISKK243 pKa = 10.11HH244 pKa = 4.6YY245 pKa = 10.66DD246 pKa = 3.46GINRR250 pKa = 11.84SMIYY254 pKa = 10.72DD255 pKa = 3.79DD256 pKa = 4.44VEE258 pKa = 5.62AGAGPKK264 pKa = 8.62ATNSIASGTPYY275 pKa = 11.34GNMGDD280 pKa = 4.75LFVLDD285 pKa = 4.95FFSSASDD292 pKa = 4.04DD293 pKa = 3.89EE294 pKa = 4.71TSGATFQPHH303 pKa = 4.72GRR305 pKa = 11.84YY306 pKa = 8.68YY307 pKa = 8.23WHH309 pKa = 7.47EE310 pKa = 4.37GQAHH314 pKa = 6.07

Molecular weight: 35.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

652

314

338

326.0

37.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.675 ± 0.266

1.227 ± 0.418

7.362 ± 0.703

3.681 ± 0.803

5.521 ± 1.204

8.436 ± 0.116

3.528 ± 0.694

4.294 ± 0.342

3.834 ± 0.911

5.061 ± 0.878

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.994 ± 0.392

3.988 ± 0.334

4.448 ± 0.684

3.681 ± 0.577

10.583 ± 1.753

8.282 ± 1.126

5.982 ± 1.628

4.755 ± 0.241

2.454 ± 0.836

5.215 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski