Avian metapneumovirus type D

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae; Metapneumovirus; Avian metapneumovirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077SG97|A0A077SG97_9MONO Small hydrophobic protein OS=Avian metapneumovirus type D OX=519376 GN=SH PE=4 SV=1
MM1 pKa = 7.55SFPEE5 pKa = 4.55GKK7 pKa = 9.88DD8 pKa = 2.94ILMMGSEE15 pKa = 4.21AAKK18 pKa = 10.53LADD21 pKa = 4.64AYY23 pKa = 10.85QKK25 pKa = 10.73SLKK28 pKa = 10.65DD29 pKa = 3.26PSRR32 pKa = 11.84NSKK35 pKa = 10.48SISGDD40 pKa = 3.12PVATVSEE47 pKa = 4.43RR48 pKa = 11.84VPTLPLCGPISPKK61 pKa = 10.54GSSTKK66 pKa = 8.65PTRR69 pKa = 11.84EE70 pKa = 3.66ASPAPKK76 pKa = 8.78KK77 pKa = 9.24TGPIYY82 pKa = 10.25PKK84 pKa = 10.66LPTAPPDD91 pKa = 4.01PGQPEE96 pKa = 5.27PITSNGPKK104 pKa = 9.9KK105 pKa = 10.24AQKK108 pKa = 9.76KK109 pKa = 8.26VKK111 pKa = 10.08FDD113 pKa = 3.34AVKK116 pKa = 9.64PGKK119 pKa = 9.76YY120 pKa = 9.73SKK122 pKa = 11.09LEE124 pKa = 3.99EE125 pKa = 4.16EE126 pKa = 4.31ALEE129 pKa = 4.25LLSDD133 pKa = 4.23PDD135 pKa = 3.71EE136 pKa = 4.92EE137 pKa = 4.27NDD139 pKa = 3.8KK140 pKa = 10.95EE141 pKa = 4.56SSILTFEE148 pKa = 4.56EE149 pKa = 4.09KK150 pKa = 10.8DD151 pKa = 3.5NASTSIEE158 pKa = 3.89ARR160 pKa = 11.84LEE162 pKa = 4.34AIEE165 pKa = 4.92EE166 pKa = 4.24KK167 pKa = 10.89LSMILGMLKK176 pKa = 9.1TLSIATAGPTAARR189 pKa = 11.84DD190 pKa = 4.19GIRR193 pKa = 11.84DD194 pKa = 3.36AMVGMRR200 pKa = 11.84EE201 pKa = 3.93EE202 pKa = 5.1LINSIMAEE210 pKa = 3.98AKK212 pKa = 10.55DD213 pKa = 4.7KK214 pKa = 10.4ISEE217 pKa = 4.27MIKK220 pKa = 10.67EE221 pKa = 4.18EE222 pKa = 3.77DD223 pKa = 3.39TQRR226 pKa = 11.84AKK228 pKa = 10.66IGDD231 pKa = 3.48GSVRR235 pKa = 11.84LTEE238 pKa = 4.4KK239 pKa = 10.74AKK241 pKa = 10.41EE242 pKa = 3.96LNKK245 pKa = 10.04VLEE248 pKa = 4.79DD249 pKa = 3.49QSSSGEE255 pKa = 3.99SDD257 pKa = 3.5SEE259 pKa = 4.72SEE261 pKa = 4.5DD262 pKa = 3.81EE263 pKa = 4.39EE264 pKa = 4.6PEE266 pKa = 4.06PDD268 pKa = 3.46EE269 pKa = 4.26TADD272 pKa = 3.54IYY274 pKa = 11.54SLEE277 pKa = 4.07LL278 pKa = 4.26

Molecular weight: 30.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077SJ11|A0A077SJ11_9MONO Matrix protein 2-2 OS=Avian metapneumovirus type D OX=519376 GN=M2.2 PE=4 SV=1
MM1 pKa = 7.68SKK3 pKa = 10.5RR4 pKa = 11.84NPCRR8 pKa = 11.84YY9 pKa = 9.76EE10 pKa = 3.46IRR12 pKa = 11.84GKK14 pKa = 9.97CNRR17 pKa = 11.84GSSCSFNHH25 pKa = 6.62NYY27 pKa = 9.05WSWPDD32 pKa = 3.23HH33 pKa = 5.68VLLVRR38 pKa = 11.84INCMLNQLQRR48 pKa = 11.84NTDD51 pKa = 3.42RR52 pKa = 11.84TDD54 pKa = 3.29GLSLISGAGRR64 pKa = 11.84EE65 pKa = 4.33DD66 pKa = 3.38RR67 pKa = 11.84TQDD70 pKa = 3.55FVLGSANVVQGYY82 pKa = 10.09IDD84 pKa = 4.0GNATITKK91 pKa = 9.99SAACYY96 pKa = 10.26SLYY99 pKa = 10.99NIIKK103 pKa = 9.6QLQEE107 pKa = 3.8NDD109 pKa = 3.15VKK111 pKa = 11.08ASRR114 pKa = 11.84DD115 pKa = 3.5TMLDD119 pKa = 3.33DD120 pKa = 4.53HH121 pKa = 6.76KK122 pKa = 10.69HH123 pKa = 4.69VALHH127 pKa = 6.1NLVLSYY133 pKa = 10.97IDD135 pKa = 3.46MSKK138 pKa = 10.78NPASLINSLKK148 pKa = 10.5RR149 pKa = 11.84LPKK152 pKa = 10.42EE153 pKa = 3.91KK154 pKa = 10.38LKK156 pKa = 11.01KK157 pKa = 8.17LAKK160 pKa = 10.54VIVQLSAGPEE170 pKa = 3.82VEE172 pKa = 4.32NASGHH177 pKa = 5.82PMQKK181 pKa = 10.09GDD183 pKa = 4.43SGHH186 pKa = 5.42QVV188 pKa = 2.65

Molecular weight: 21.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4292

73

2006

476.9

53.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.757 ± 0.943

2.586 ± 0.601

4.497 ± 0.313

5.988 ± 0.624

2.912 ± 0.604

5.685 ± 0.377

1.678 ± 0.381

6.431 ± 0.36

6.267 ± 0.489

9.646 ± 0.9

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.889 ± 0.306

4.939 ± 0.695

4.497 ± 0.891

3.518 ± 0.376

5.336 ± 0.441

8.201 ± 0.469

7.106 ± 0.996

6.99 ± 0.647

0.955 ± 0.309

3.122 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski