Bdellovibrio sp. SKB1291214

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bdellovibrionales; Bdellovibrionaceae; Bdellovibrio; unclassified Bdellovibrio

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A254QHZ5|A0A254QHZ5_9PROT DUF4105 domain-containing protein OS=Bdellovibrio sp. SKB1291214 OX=1732569 GN=B9G69_09260 PE=4 SV=1
MM1 pKa = 6.78VHH3 pKa = 6.6SKK5 pKa = 8.57FTAVLAGLGFLATLSACSAQSDD27 pKa = 3.69KK28 pKa = 11.52DD29 pKa = 3.66KK30 pKa = 11.48LMEE33 pKa = 4.31AQFCLDD39 pKa = 3.42EE40 pKa = 4.47STAATAQSCVSSIAYY55 pKa = 7.35MQTSQSYY62 pKa = 10.84ALQCAAGFIEE72 pKa = 5.18SGVTSPANLSQALDD86 pKa = 3.98SISNNSNSSTALLSALNMGSVSLANNTAEE115 pKa = 4.16YY116 pKa = 9.48CAKK119 pKa = 10.07SGQVGFSLLGAMAKK133 pKa = 9.93SATSIASAASSLGLGSCSTDD153 pKa = 3.48LSQCDD158 pKa = 3.29TSQIEE163 pKa = 4.14DD164 pKa = 4.56TITEE168 pKa = 3.91IQKK171 pKa = 9.2TINSDD176 pKa = 3.35PSATMSLADD185 pKa = 3.23ATAAVEE191 pKa = 4.98AIASSIQTVYY201 pKa = 8.05TTTCGTTGANQDD213 pKa = 2.78ICDD216 pKa = 4.81PIKK219 pKa = 9.98TAIADD224 pKa = 3.74AGVDD228 pKa = 3.21ITDD231 pKa = 4.1PNLDD235 pKa = 3.47LVALGKK241 pKa = 10.27EE242 pKa = 4.97LLAQWKK248 pKa = 8.92PP249 pKa = 3.3

Molecular weight: 25.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A254QM87|A0A254QM87_9PROT Ribonuclease H OS=Bdellovibrio sp. SKB1291214 OX=1732569 GN=rnhA PE=3 SV=1
MM1 pKa = 7.68NIQEE5 pKa = 4.3NPQRR9 pKa = 11.84APRR12 pKa = 11.84KK13 pKa = 9.43RR14 pKa = 11.84EE15 pKa = 3.7APVPLRR21 pKa = 11.84GPSQDD26 pKa = 2.85LFKK29 pKa = 10.73RR30 pKa = 11.84SKK32 pKa = 10.47QEE34 pKa = 3.54LSFSVGTGFQGGPSRR49 pKa = 11.84KK50 pKa = 8.98RR51 pKa = 11.84KK52 pKa = 9.59GYY54 pKa = 10.5RR55 pKa = 11.84LALWSLMASFIDD67 pKa = 4.39ALILLAISCAFLVLFSLLMKK87 pKa = 10.06TPANAVIKK95 pKa = 10.41IFFRR99 pKa = 11.84SEE101 pKa = 4.0SNIMLLAQLYY111 pKa = 9.77AVSAWVYY118 pKa = 10.21LIATRR123 pKa = 11.84VLTGSSIGEE132 pKa = 3.94WACNIRR138 pKa = 11.84LGQPHH143 pKa = 6.41EE144 pKa = 4.5RR145 pKa = 11.84MEE147 pKa = 4.02TRR149 pKa = 11.84YY150 pKa = 9.18VWRR153 pKa = 11.84VLKK156 pKa = 10.66RR157 pKa = 11.84STLIVATGVVVLPALSLILGGDD179 pKa = 3.75LTGKK183 pKa = 10.31LCGLRR188 pKa = 11.84LFSLKK193 pKa = 10.66

Molecular weight: 21.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3588

0

3588

1113114

37

2152

310.2

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.31 ± 0.039

0.918 ± 0.014

5.168 ± 0.027

6.234 ± 0.048

4.573 ± 0.034

6.938 ± 0.045

1.911 ± 0.02

6.081 ± 0.033

6.948 ± 0.049

9.463 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.019

4.343 ± 0.026

4.045 ± 0.026

3.954 ± 0.026

4.529 ± 0.031

6.972 ± 0.044

5.581 ± 0.053

6.959 ± 0.035

1.181 ± 0.016

3.091 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski