Pandoravirus dulcis
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1070 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4VTA8|S4VTA8_9VIRU Fascin-like protein OS=Pandoravirus dulcis OX=1349409 GN=pdul_cds_543 PE=4 SV=1
MM1 pKa = 7.02 ATCSGSPQDD10 pKa = 5.88 DD11 pKa = 3.9 EE12 pKa = 4.94 CCPITLVPFRR22 pKa = 11.84 TITAARR28 pKa = 11.84 TTFLPCCHH36 pKa = 6.87 RR37 pKa = 11.84 FDD39 pKa = 4.22 SDD41 pKa = 4.8 AIRR44 pKa = 11.84 HH45 pKa = 5.69 YY46 pKa = 10.81 IDD48 pKa = 3.23 NCLARR53 pKa = 11.84 RR54 pKa = 11.84 APRR57 pKa = 11.84 GAAPPVPCPVCRR69 pKa = 11.84 CRR71 pKa = 11.84 VPPDD75 pKa = 3.46 YY76 pKa = 10.66 LADD79 pKa = 3.51 MGVPVAWPPSAPVVAPVAAPAVPAAPAVPAAPAASAAIAVAAPATTARR127 pKa = 11.84 RR128 pKa = 11.84 SSRR131 pKa = 11.84 PSRR134 pKa = 11.84 PPAPVVIVISDD145 pKa = 4.03 DD146 pKa = 4.37 DD147 pKa = 4.73 DD148 pKa = 6.09 DD149 pKa = 5.27 SEE151 pKa = 4.5 EE152 pKa = 4.07 VHH154 pKa = 6.51 EE155 pKa = 5.83 RR156 pKa = 11.84 TAQWINSDD164 pKa = 4.8 DD165 pKa = 5.32 DD166 pKa = 5.72 DD167 pKa = 7.53 DD168 pKa = 7.46 GDD170 pKa = 6.17 DD171 pKa = 6.01 DD172 pKa = 7.01 DD173 pKa = 7.52 DD174 pKa = 6.31 DD175 pKa = 6.45 DD176 pKa = 4.38 STGSLDD182 pKa = 3.92 EE183 pKa = 5.41 FIVDD187 pKa = 5.3 DD188 pKa = 6.21 DD189 pKa = 4.2 EE190 pKa = 4.64 TDD192 pKa = 3.39 PDD194 pKa = 4.2 AEE196 pKa = 4.29 YY197 pKa = 10.38 TPAPVVAAPDD207 pKa = 4.41 DD208 pKa = 5.4 DD209 pKa = 6.0 DD210 pKa = 7.58 DD211 pKa = 7.08 SDD213 pKa = 6.5 LDD215 pKa = 5.58 DD216 pKa = 6.79 DD217 pKa = 6.31 DD218 pKa = 7.05 DD219 pKa = 6.44 DD220 pKa = 4.89 STDD223 pKa = 3.76 SDD225 pKa = 4.8 CSCLTDD231 pKa = 4.66 SEE233 pKa = 5.35 DD234 pKa = 3.16 II235 pKa = 4.24
Molecular weight: 24.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.881
IPC2_protein 3.541
IPC_protein 3.605
Toseland 3.35
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.605
Rodwell 3.414
Grimsley 3.261
Solomon 3.617
Lehninger 3.567
Nozaki 3.732
DTASelect 4.062
Thurlkill 3.427
EMBOSS 3.605
Sillero 3.732
Patrickios 2.486
IPC_peptide 3.605
IPC2_peptide 3.694
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|S4VSA2|S4VSA2_9VIRU Uncharacterized protein OS=Pandoravirus dulcis OX=1349409 GN=pdul_cds_935 PE=4 SV=1
MM1 pKa = 7.97 GDD3 pKa = 3.41 RR4 pKa = 11.84 PDD6 pKa = 4.5 RR7 pKa = 11.84 GSKK10 pKa = 8.38 CTRR13 pKa = 11.84 APAFVVGSCVCTEE26 pKa = 4.14 RR27 pKa = 11.84 EE28 pKa = 4.26 VRR30 pKa = 11.84 WSFFLLVVILTLVTISLVLFVSAVRR55 pKa = 11.84 YY56 pKa = 7.57 VKK58 pKa = 10.34 SWVSEE63 pKa = 4.08 KK64 pKa = 10.33 QRR66 pKa = 11.84 LRR68 pKa = 11.84 ADD70 pKa = 3.43 VKK72 pKa = 10.86 ALSRR76 pKa = 11.84 QASGVMRR83 pKa = 11.84 DD84 pKa = 3.48 AQQRR88 pKa = 11.84 AAAAASAPSHH98 pKa = 6.56 VPDD101 pKa = 4.13 ATATAPPARR110 pKa = 11.84 APPSAAEE117 pKa = 3.8 SAAASKK123 pKa = 10.96 ARR125 pKa = 11.84 TAAVAAADD133 pKa = 3.98 PLVHH137 pKa = 6.59 EE138 pKa = 4.82 MLDD141 pKa = 3.61 AIEE144 pKa = 4.02 AVRR147 pKa = 11.84 LGSCGRR153 pKa = 11.84 GFAFAVDD160 pKa = 3.53 SRR162 pKa = 11.84 GGVWAHH168 pKa = 5.67 GKK170 pKa = 7.39 TRR172 pKa = 11.84 HH173 pKa = 5.65 LARR176 pKa = 11.84 GATGRR181 pKa = 11.84 VPGFHH186 pKa = 6.56 RR187 pKa = 11.84 VADD190 pKa = 4.1 ADD192 pKa = 3.8 HH193 pKa = 6.93 TGGSTSGAYY202 pKa = 10.22 DD203 pKa = 3.89 CDD205 pKa = 4.41 DD206 pKa = 3.89 DD207 pKa = 5.93 HH208 pKa = 7.06 EE209 pKa = 7.06 ADD211 pKa = 3.26 ASVYY215 pKa = 10.54 GGAPVARR222 pKa = 11.84 GRR224 pKa = 11.84 RR225 pKa = 11.84 RR226 pKa = 11.84 RR227 pKa = 11.84 GGAPARR233 pKa = 11.84 APSPLADD240 pKa = 3.4 MLGAARR246 pKa = 11.84 RR247 pKa = 11.84 GGGYY251 pKa = 7.49 VHH253 pKa = 6.79 FRR255 pKa = 11.84 WKK257 pKa = 10.73 RR258 pKa = 11.84 EE259 pKa = 4.0 VLMMAYY265 pKa = 8.59 VHH267 pKa = 6.15 PVKK270 pKa = 10.37 GTDD273 pKa = 3.4 LVLGGAVPVPRR284 pKa = 11.84 KK285 pKa = 9.16 QKK287 pKa = 10.36 AWEE290 pKa = 3.96 EE291 pKa = 4.05 ANAARR296 pKa = 11.84 PAAPARR302 pKa = 11.84 RR303 pKa = 3.79
Molecular weight: 31.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.458
IPC_protein 10.218
Toseland 10.526
ProMoST 10.365
Dawson 10.628
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 10.76
Grimsley 10.687
Solomon 10.745
Lehninger 10.716
Nozaki 10.54
DTASelect 10.35
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.57
Patrickios 10.467
IPC_peptide 10.76
IPC2_peptide 9.502
IPC2.peptide.svr19 8.671
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1070
0
1070
424486
60
2245
396.7
42.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.358 ± 0.097
2.433 ± 0.044
7.623 ± 0.079
3.921 ± 0.049
2.213 ± 0.033
8.227 ± 0.071
2.788 ± 0.046
2.809 ± 0.031
1.879 ± 0.048
7.964 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.911 ± 0.026
1.84 ± 0.031
6.873 ± 0.081
2.469 ± 0.039
9.165 ± 0.069
5.629 ± 0.062
5.51 ± 0.056
7.519 ± 0.064
1.863 ± 0.036
2.005 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here