Hubei permutotetra-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KI14|A0A1L3KI14_9VIRU Uncharacterized protein OS=Hubei permutotetra-like virus 3 OX=1923077 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 5.6ASNPVNSAEE11 pKa = 3.96RR12 pKa = 11.84FTLEE16 pKa = 3.62EE17 pKa = 3.7MRR19 pKa = 11.84FEE21 pKa = 4.26HH22 pKa = 6.63LRR24 pKa = 11.84KK25 pKa = 9.59KK26 pKa = 10.29RR27 pKa = 11.84QSLQALQDD35 pKa = 3.58MAQSAEE41 pKa = 3.5RR42 pKa = 11.84HH43 pKa = 4.75YY44 pKa = 11.24FGDD47 pKa = 3.22GLYY50 pKa = 10.94NNEE53 pKa = 3.68RR54 pKa = 11.84YY55 pKa = 10.2SRR57 pKa = 11.84VFEE60 pKa = 4.38ALEE63 pKa = 3.88KK64 pKa = 9.99ATPHH68 pKa = 6.19FDD70 pKa = 3.0WYY72 pKa = 11.03AEE74 pKa = 3.96PWDD77 pKa = 4.0LSFLKK82 pKa = 10.33KK83 pKa = 9.86MPPIPVDD90 pKa = 3.44QTKK93 pKa = 10.75HH94 pKa = 4.4EE95 pKa = 4.4TLQIHH100 pKa = 6.87PPNKK104 pKa = 9.93NIAGGYY110 pKa = 8.95VSTSRR115 pKa = 11.84IFGSPQGLDD124 pKa = 2.82KK125 pKa = 11.44GLIQGLSQVFNEE137 pKa = 4.91DD138 pKa = 3.13EE139 pKa = 4.64LIEE142 pKa = 4.74AGSKK146 pKa = 9.9YY147 pKa = 10.07CYY149 pKa = 10.01CYY151 pKa = 10.49GSRR154 pKa = 11.84GGFQKK159 pKa = 10.45RR160 pKa = 11.84LKK162 pKa = 10.17MLMTRR167 pKa = 11.84QCKK170 pKa = 9.83SPYY173 pKa = 9.29TPPQVATKK181 pKa = 10.26LKK183 pKa = 9.77RR184 pKa = 11.84LMPRR188 pKa = 11.84GPLPNWHH195 pKa = 6.78EE196 pKa = 4.13FDD198 pKa = 4.42AMMEE202 pKa = 4.21AVTITAGASAGAPYY216 pKa = 10.17WKK218 pKa = 10.09DD219 pKa = 3.07KK220 pKa = 10.87RR221 pKa = 11.84DD222 pKa = 3.72ALHH225 pKa = 6.55EE226 pKa = 4.27CVGTILPVVVEE237 pKa = 4.07ALNDD241 pKa = 3.8GSLQTLMNGRR251 pKa = 11.84PEE253 pKa = 3.89WFLVEE258 pKa = 4.67IKK260 pKa = 10.89NKK262 pKa = 9.58LDD264 pKa = 3.39RR265 pKa = 11.84YY266 pKa = 9.55EE267 pKa = 3.99VTKK270 pKa = 10.84LGEE273 pKa = 3.74KK274 pKa = 9.87CRR276 pKa = 11.84PYY278 pKa = 11.43GNMPMHH284 pKa = 6.99LSLLFSALIQPFCKK298 pKa = 10.13ALHH301 pKa = 5.75VWTKK305 pKa = 11.19SDD307 pKa = 3.06TCNAYY312 pKa = 10.3GMTMCHH318 pKa = 6.66AEE320 pKa = 4.25LYY322 pKa = 9.99SWTSRR327 pKa = 11.84IRR329 pKa = 11.84QTALKK334 pKa = 10.04AIKK337 pKa = 8.88TKK339 pKa = 10.37KK340 pKa = 7.14PHH342 pKa = 5.88YY343 pKa = 8.83MFGVYY348 pKa = 10.5GDD350 pKa = 4.18DD351 pKa = 4.99GEE353 pKa = 4.86ILRR356 pKa = 11.84CMKK359 pKa = 10.66DD360 pKa = 3.17GTVYY364 pKa = 10.91AVDD367 pKa = 3.96PDD369 pKa = 4.49FKK371 pKa = 11.52QMDD374 pKa = 3.72GSVDD378 pKa = 3.56YY379 pKa = 8.94NTVKK383 pKa = 10.55GVCKK387 pKa = 10.39WIGDD391 pKa = 4.07SFAKK395 pKa = 10.19EE396 pKa = 4.29HH397 pKa = 6.78GDD399 pKa = 3.6SPFWRR404 pKa = 11.84IVLDD408 pKa = 4.3LLAEE412 pKa = 4.25MATNPLMIIDD422 pKa = 4.34GRR424 pKa = 11.84DD425 pKa = 3.24TYY427 pKa = 11.3RR428 pKa = 11.84KK429 pKa = 8.16NQKK432 pKa = 10.23DD433 pKa = 4.04GIISGVVGTTLFDD446 pKa = 3.42TAKK449 pKa = 10.79AAIAYY454 pKa = 9.82SDD456 pKa = 4.32YY457 pKa = 11.11IEE459 pKa = 4.1QCEE462 pKa = 4.04YY463 pKa = 11.38DD464 pKa = 5.08LSLFDD469 pKa = 3.73NQDD472 pKa = 2.83RR473 pKa = 11.84SVKK476 pKa = 9.88YY477 pKa = 10.23FRR479 pKa = 11.84EE480 pKa = 3.93KK481 pKa = 10.51HH482 pKa = 4.97GLEE485 pKa = 4.13VKK487 pKa = 10.29EE488 pKa = 4.25GTWRR492 pKa = 11.84KK493 pKa = 7.24TVLNMNPDD501 pKa = 3.21NAEE504 pKa = 3.67QLFRR508 pKa = 11.84EE509 pKa = 4.59TKK511 pKa = 10.02FLGMQLKK518 pKa = 8.65WVYY521 pKa = 10.62DD522 pKa = 3.91PSFDD526 pKa = 4.47GPILVPSLPTRR537 pKa = 11.84DD538 pKa = 3.34WLEE541 pKa = 4.04LLTTPRR547 pKa = 11.84DD548 pKa = 3.47NAEE551 pKa = 4.23TKK553 pKa = 10.4KK554 pKa = 10.63KK555 pKa = 10.07EE556 pKa = 4.3SNITHH561 pKa = 5.54QRR563 pKa = 11.84RR564 pKa = 11.84TFDD567 pKa = 2.88RR568 pKa = 11.84CRR570 pKa = 11.84GLLITGAVWDD580 pKa = 4.44PDD582 pKa = 3.78VAEE585 pKa = 4.58ALYY588 pKa = 9.25MAIDD592 pKa = 3.93QVPAAAILMEE602 pKa = 4.13VQAKK606 pKa = 9.18KK607 pKa = 11.0GKK609 pKa = 9.99GAVEE613 pKa = 3.99AVNDD617 pKa = 5.02LILNEE622 pKa = 4.63DD623 pKa = 3.65FSFPSSEE630 pKa = 3.92GVPLRR635 pKa = 11.84SWVAALYY642 pKa = 11.01ALTEE646 pKa = 4.14EE647 pKa = 4.63EE648 pKa = 4.48KK649 pKa = 11.06EE650 pKa = 4.3QINSDD655 pKa = 4.14DD656 pKa = 3.85PFSIAFQPVYY666 pKa = 10.32PSLADD671 pKa = 4.32FIRR674 pKa = 11.84NRR676 pKa = 11.84KK677 pKa = 7.96DD678 pKa = 2.7TWKK681 pKa = 10.27EE682 pKa = 3.04RR683 pKa = 11.84WKK685 pKa = 11.24FMMKK689 pKa = 10.23PSTHH693 pKa = 6.38PQLEE697 pKa = 4.18VSLKK701 pKa = 7.56YY702 pKa = 9.4TEE704 pKa = 4.12YY705 pKa = 11.24DD706 pKa = 3.48FVNTEE711 pKa = 4.23PPLPPIPVASTSFAKK726 pKa = 10.08PLKK729 pKa = 9.95KK730 pKa = 10.45VIIMEE735 pKa = 4.01PFKK738 pKa = 10.61TKK740 pKa = 10.3KK741 pKa = 9.78HH742 pKa = 6.6DD743 pKa = 3.72GDD745 pKa = 3.98EE746 pKa = 4.37VQVAIAPKK754 pKa = 10.2QSLQSIKK761 pKa = 10.58EE762 pKa = 4.07YY763 pKa = 9.81TEE765 pKa = 3.46KK766 pKa = 11.27AMIKK770 pKa = 10.53DD771 pKa = 3.66PTPFMAAIAPHH782 pKa = 6.93LEE784 pKa = 3.92TTGIDD789 pKa = 3.27RR790 pKa = 11.84EE791 pKa = 4.4KK792 pKa = 10.44VVEE795 pKa = 3.97YY796 pKa = 10.54AKK798 pKa = 10.71NYY800 pKa = 8.42SWVVDD805 pKa = 3.85RR806 pKa = 11.84EE807 pKa = 4.18PFDD810 pKa = 3.43RR811 pKa = 11.84TFVMPQLNAADD822 pKa = 3.89APKK825 pKa = 10.35QIVRR829 pKa = 11.84RR830 pKa = 11.84FVEE833 pKa = 4.49EE834 pKa = 3.74NGLEE838 pKa = 4.39YY839 pKa = 10.83VSPPCVTLDD848 pKa = 3.15QRR850 pKa = 11.84TDD852 pKa = 3.25LKK854 pKa = 10.6EE855 pKa = 3.69QTVEE859 pKa = 4.15VRR861 pKa = 11.84DD862 pKa = 3.93PLTNNQIQLARR873 pKa = 11.84LRR875 pKa = 11.84TLGVRR880 pKa = 11.84AKK882 pKa = 10.98VMEE885 pKa = 4.04QVINEE890 pKa = 3.88QFATYY895 pKa = 10.56LRR897 pKa = 11.84TLEE900 pKa = 4.59DD901 pKa = 3.36VLPRR905 pKa = 11.84SRR907 pKa = 11.84DD908 pKa = 3.64DD909 pKa = 3.99SPTSSWKK916 pKa = 10.62AIADD920 pKa = 3.86AEE922 pKa = 4.2AVEE925 pKa = 4.83ADD927 pKa = 3.6TSVVSSVSTSQAARR941 pKa = 11.84ITKK944 pKa = 10.0DD945 pKa = 3.04IINASIVALKK955 pKa = 10.64KK956 pKa = 10.14EE957 pKa = 4.1LSAFITTEE965 pKa = 4.01LNKK968 pKa = 9.84QLKK971 pKa = 9.76KK972 pKa = 10.43EE973 pKa = 4.18RR974 pKa = 11.84EE975 pKa = 4.02KK976 pKa = 11.04HH977 pKa = 5.74ASTTAKK983 pKa = 10.29KK984 pKa = 10.01KK985 pKa = 10.5RR986 pKa = 11.84KK987 pKa = 8.74NGKK990 pKa = 9.2NGGSSRR996 pKa = 11.84KK997 pKa = 7.64TEE999 pKa = 4.02EE1000 pKa = 4.1VPSGSRR1006 pKa = 11.84NQTSKK1011 pKa = 10.44SSSRR1015 pKa = 11.84RR1016 pKa = 11.84ASDD1019 pKa = 3.5DD1020 pKa = 3.96SEE1022 pKa = 4.62SHH1024 pKa = 6.33SPPSS1028 pKa = 3.27

Molecular weight: 116.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KI14|A0A1L3KI14_9VIRU Uncharacterized protein OS=Hubei permutotetra-like virus 3 OX=1923077 PE=4 SV=1
MM1 pKa = 7.71PAQLRR6 pKa = 11.84KK7 pKa = 10.11RR8 pKa = 11.84NVKK11 pKa = 5.62TARR14 pKa = 11.84MVVAPAKK21 pKa = 9.96RR22 pKa = 11.84KK23 pKa = 9.69RR24 pKa = 11.84SPRR27 pKa = 11.84DD28 pKa = 2.98QGTKK32 pKa = 10.46LLNQARR38 pKa = 11.84DD39 pKa = 4.02GLRR42 pKa = 11.84MTAKK46 pKa = 10.57AIVHH50 pKa = 6.21PLSIVMFIFSLYY62 pKa = 10.48LFYY65 pKa = 11.57NNAPLTNFATKK76 pKa = 10.53LEE78 pKa = 4.31SSALLKK84 pKa = 10.64PLGEE88 pKa = 4.52FIKK91 pKa = 9.43THH93 pKa = 4.73VLQVAGLVWIIPVTFIAARR112 pKa = 11.84PAWAFLASLVSSIFVTEE129 pKa = 3.97YY130 pKa = 10.88LKK132 pKa = 10.14TPTKK136 pKa = 7.96YY137 pKa = 9.26WEE139 pKa = 4.0YY140 pKa = 11.2AFLGISATMLLRR152 pKa = 11.84ARR154 pKa = 11.84TGVQFFIAVLLVVLSLGLGLWGDD177 pKa = 4.08EE178 pKa = 4.44LFAPPAGAPEE188 pKa = 4.47EE189 pKa = 4.55PPAVTPPSGSS199 pKa = 3.16

Molecular weight: 21.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1433

199

1028

477.7

53.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.234 ± 1.031

1.256 ± 0.368

5.094 ± 1.774

6.141 ± 1.284

4.257 ± 0.628

4.745 ± 0.376

1.884 ± 0.299

5.024 ± 0.728

6.909 ± 1.527

9.7 ± 2.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.407

3.419 ± 0.371

5.932 ± 0.733

3.629 ± 0.48

5.304 ± 0.429

7.258 ± 0.976

6.769 ± 0.76

6.42 ± 0.404

1.535 ± 0.443

3.838 ± 1.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski