Pectobacterium phage PhiM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Phimunavirus; Pectobacterium virus fM1

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P7WFU5|A0A1P7WFU5_9CAUD Uncharacterized protein OS=Pectobacterium phage PhiM1 OX=1211386 GN=PhiM1_04 PE=4 SV=1
MM1 pKa = 7.63TYY3 pKa = 10.34YY4 pKa = 10.64EE5 pKa = 4.26SADD8 pKa = 3.72VIITRR13 pKa = 11.84ARR15 pKa = 11.84AVQEE19 pKa = 3.92LRR21 pKa = 11.84RR22 pKa = 11.84HH23 pKa = 6.67GITDD27 pKa = 3.43TAEE30 pKa = 4.04FDD32 pKa = 3.64SDD34 pKa = 3.38MGICTEE40 pKa = 4.26YY41 pKa = 10.78NAQDD45 pKa = 3.39VLQWLGYY52 pKa = 10.06

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P7WFU2|A0A1P7WFU2_9CAUD Uncharacterized protein OS=Pectobacterium phage PhiM1 OX=1211386 GN=PhiM1_01 PE=4 SV=1
MM1 pKa = 7.67SYY3 pKa = 10.98AKK5 pKa = 10.36KK6 pKa = 9.81LQKK9 pKa = 10.7SFTGLSQLQGGALQKK24 pKa = 10.68RR25 pKa = 11.84RR26 pKa = 11.84DD27 pKa = 3.87KK28 pKa = 11.25LNQAGVHH35 pKa = 5.77HH36 pKa = 6.98EE37 pKa = 4.13KK38 pKa = 10.53QRR40 pKa = 11.84ATFDD44 pKa = 3.36TNTRR48 pKa = 11.84ATEE51 pKa = 3.83KK52 pKa = 10.52RR53 pKa = 11.84RR54 pKa = 11.84GCSKK58 pKa = 10.42PPRR61 pKa = 11.84GLL63 pKa = 3.38

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13541

36

1263

260.4

28.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.874 ± 0.514

1.196 ± 0.188

6.196 ± 0.235

5.125 ± 0.313

2.917 ± 0.154

7.71 ± 0.251

1.846 ± 0.218

4.542 ± 0.214

4.446 ± 0.333

8.722 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.197

4.549 ± 0.271

3.848 ± 0.205

4.911 ± 0.403

5.457 ± 0.248

6.646 ± 0.28

6.691 ± 0.497

7.607 ± 0.228

1.344 ± 0.141

3.848 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski