Thermoanaerobacteraceae bacterium SP2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; unclassified Thermoanaerobacteraceae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3424 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9K1L8|A0A3A9K1L8_9THEO Pyridoxal-phosphate dependent enzyme OS=Thermoanaerobacteraceae bacterium SP2 OX=2292448 GN=DXT63_03225 PE=4 SV=1
MM1 pKa = 7.7ALTYY5 pKa = 8.42TASSTGSTLQTANVAIAVSPASGVLSVTAMLPGDD39 pKa = 3.8SATAVINVSNTGDD52 pKa = 3.06VDD54 pKa = 3.44EE55 pKa = 5.41YY56 pKa = 11.38YY57 pKa = 10.63FVSADD62 pKa = 3.11WKK64 pKa = 10.49ASPGTTTSHH73 pKa = 7.1AALLADD79 pKa = 3.91NLNVSVSASPGGSIYY94 pKa = 10.2TGKK97 pKa = 10.73LSGLIDD103 pKa = 4.07RR104 pKa = 11.84PDD106 pKa = 3.48SPGQALALTTGNEE119 pKa = 4.08DD120 pKa = 3.48VTFTFYY126 pKa = 11.17LPSTAGNVVGNIDD139 pKa = 3.51MTLDD143 pKa = 3.64FVFVATSS150 pKa = 2.99

Molecular weight: 15.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9JUD2|A0A3A9JUD2_9THEO Uncharacterized protein OS=Thermoanaerobacteraceae bacterium SP2 OX=2292448 GN=DXT63_14005 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 9.29QTYY5 pKa = 9.14QPSRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 8.88RR12 pKa = 11.84KK13 pKa = 9.33KK14 pKa = 8.81VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.15RR21 pKa = 11.84MMTKK25 pKa = 10.0NGRR28 pKa = 11.84NVIRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3424

0

3424

1012711

21

2758

295.8

32.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.415 ± 0.046

1.054 ± 0.017

5.304 ± 0.034

7.165 ± 0.041

4.183 ± 0.03

7.529 ± 0.037

1.563 ± 0.016

8.654 ± 0.037

7.546 ± 0.045

9.405 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.023

4.291 ± 0.027

3.942 ± 0.028

2.78 ± 0.03

4.626 ± 0.031

5.552 ± 0.025

4.78 ± 0.029

7.187 ± 0.034

0.791 ± 0.012

3.349 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski