Bovine papillomavirus type 1
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P0DOD6|VE8E2_BPV1 Protein E8^E2C OS=Bovine papillomavirus type 1 OX=337052 PE=3 SV=1
MM1 pKa = 7.49 PNLWFLLFLGLVAAMQLLLLLFLLLFFLVYY31 pKa = 9.25 WDD33 pKa = 4.88 HH34 pKa = 7.04 FEE36 pKa = 4.31 CSCTGLPFF44 pKa = 4.63
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.503
IPC2_protein 4.876
IPC_protein 4.151
Toseland 4.062
ProMoST 4.393
Dawson 4.177
Bjellqvist 4.355
Wikipedia 4.177
Rodwell 4.05
Grimsley 4.012
Solomon 4.101
Lehninger 4.062
Nozaki 4.38
DTASelect 4.431
Thurlkill 4.19
EMBOSS 4.177
Sillero 4.304
Patrickios 0.006
IPC_peptide 4.113
IPC2_peptide 4.279
IPC2.peptide.svr19 4.237
Protein with the highest isoelectric point:
>sp|P06931|VE6_BPV1 Protein E6 OS=Bovine papillomavirus type 1 OX=337052 GN=E6 PE=1 SV=1
MM1 pKa = 7.56 ANDD4 pKa = 3.62 KK5 pKa = 10.62 EE6 pKa = 4.39 IAQTEE11 pKa = 4.68 SGSHH15 pKa = 6.02 PKK17 pKa = 10.02 DD18 pKa = 3.35 LKK20 pKa = 10.23 EE21 pKa = 3.88 TLQEE25 pKa = 4.37 KK26 pKa = 10.44 KK27 pKa = 10.02 PSQPSLSLLCSAPPPAVPSEE47 pKa = 4.08 QASVGYY53 pKa = 8.37 GTVLARR59 pKa = 11.84 TPTIFLQARR68 pKa = 11.84 GALFSALPPPRR79 pKa = 11.84 AGHH82 pKa = 5.27 GTGGLGIKK90 pKa = 10.01 AGRR93 pKa = 11.84 RR94 pKa = 11.84 GAVARR99 pKa = 11.84 LHH101 pKa = 6.82 RR102 pKa = 11.84 GRR104 pKa = 11.84 TSDD107 pKa = 3.37 SPKK110 pKa = 10.32 AHH112 pKa = 6.38 HH113 pKa = 5.96 QQ114 pKa = 3.35
Molecular weight: 11.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.189
IPC2_protein 9.385
IPC_protein 9.516
Toseland 10.54
ProMoST 10.028
Dawson 10.613
Bjellqvist 10.204
Wikipedia 10.716
Rodwell 11.082
Grimsley 10.628
Solomon 10.672
Lehninger 10.657
Nozaki 10.511
DTASelect 10.204
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.526
Patrickios 10.862
IPC_peptide 10.687
IPC2_peptide 8.785
IPC2.peptide.svr19 8.51
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2616
44
605
290.7
32.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.645 ± 0.566
2.408 ± 0.779
4.931 ± 0.21
6.269 ± 0.47
4.128 ± 0.578
7.607 ± 0.74
2.446 ± 0.187
3.631 ± 0.525
5.046 ± 0.736
9.557 ± 0.761
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.261 ± 0.256
3.67 ± 0.644
6.193 ± 1.146
4.014 ± 0.479
6.04 ± 0.596
8.104 ± 0.693
7.225 ± 0.833
5.543 ± 0.303
1.414 ± 0.247
2.867 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here