Propionibacterium sp. oral taxon 192 str. F0372
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2523 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S3WYA1|S3WYA1_9ACTN Uncharacterized protein OS=Propionibacterium sp. oral taxon 192 str. F0372 OX=1203605 GN=HMPREF1531_02197 PE=4 SV=1
MM1 pKa = 8.28 DD2 pKa = 4.3 IPAEE6 pKa = 3.77 LRR8 pKa = 11.84 YY9 pKa = 10.29 SIEE12 pKa = 4.24 HH13 pKa = 5.16 EE14 pKa = 4.08 WVRR17 pKa = 11.84 IDD19 pKa = 4.2 GSVARR24 pKa = 11.84 IGVTAYY30 pKa = 8.01 ATSALGDD37 pKa = 3.49 IVYY40 pKa = 10.53 VSLPTVGSSVVAQDD54 pKa = 3.11 ACGEE58 pKa = 4.16 LEE60 pKa = 4.27 STKK63 pKa = 10.73 SVSDD67 pKa = 3.41 LFAPVSGVVTSVNNAVIEE85 pKa = 4.14 NPEE88 pKa = 4.34 SIGTDD93 pKa = 3.31 PYY95 pKa = 11.37 GEE97 pKa = 4.09 GWLFDD102 pKa = 4.16 VEE104 pKa = 5.35 LSDD107 pKa = 4.36 PSQVDD112 pKa = 3.4 TLLNASGYY120 pKa = 8.97 AAHH123 pKa = 7.04 VEE125 pKa = 4.24 QQGG128 pKa = 3.09
Molecular weight: 13.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.605
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.846
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|S3WWE5|S3WWE5_9ACTN Uncharacterized protein OS=Propionibacterium sp. oral taxon 192 str. F0372 OX=1203605 GN=HMPREF1531_02292 PE=4 SV=1
MM1 pKa = 7.12 LTGTRR6 pKa = 11.84 RR7 pKa = 11.84 LHH9 pKa = 4.18 QHH11 pKa = 6.39 RR12 pKa = 11.84 GRR14 pKa = 11.84 GVRR17 pKa = 11.84 SITVPTIRR25 pKa = 11.84 RR26 pKa = 11.84 GPITRR31 pKa = 11.84 ANQPVLPGRR40 pKa = 11.84 RR41 pKa = 11.84 PAQPSLPMRR50 pKa = 11.84 DD51 pKa = 3.35 GNRR54 pKa = 11.84 PLSLLQAGTIVRR66 pKa = 11.84 PATPAQRR73 pKa = 11.84 WQFTDD78 pKa = 2.99 RR79 pKa = 11.84 GLALIMGIVAVIFGAAVVFIITQFIALGAPLAA111 pKa = 4.47
Molecular weight: 12.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.383
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.842
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2523
0
2523
805660
21
2852
319.3
34.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.439 ± 0.053
0.965 ± 0.015
6.013 ± 0.042
5.646 ± 0.043
2.964 ± 0.03
8.607 ± 0.051
2.246 ± 0.02
4.978 ± 0.033
2.527 ± 0.032
9.793 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.507 ± 0.024
2.557 ± 0.026
5.178 ± 0.041
3.361 ± 0.025
6.724 ± 0.046
6.054 ± 0.04
6.264 ± 0.044
8.677 ± 0.05
1.564 ± 0.025
1.936 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here