Streptomyces sp. AcH 505

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C2ATT9|A0A0C2ATT9_9ACTN Histidine kinase OS=Streptomyces sp. AcH 505 OX=352211 GN=HY68_12430 PE=4 SV=1
MM1 pKa = 7.67TDD3 pKa = 3.0WSQLTHH9 pKa = 7.11AYY11 pKa = 10.08GPADD15 pKa = 5.76DD16 pKa = 5.61IPALLDD22 pKa = 3.46QLASDD27 pKa = 4.09PTSEE31 pKa = 3.96RR32 pKa = 11.84WTDD35 pKa = 2.93LWSALCHH42 pKa = 5.73QGSVYY47 pKa = 10.44SASFAALPWLTRR59 pKa = 11.84VAGSEE64 pKa = 4.07DD65 pKa = 3.12QGQAVSALTLAGAIMAGADD84 pKa = 3.65QPQEE88 pKa = 3.95AAGVRR93 pKa = 11.84ARR95 pKa = 11.84YY96 pKa = 9.63ADD98 pKa = 3.85EE99 pKa = 4.5LAVLLSLANNHH110 pKa = 6.1LRR112 pKa = 11.84TADD115 pKa = 3.71DD116 pKa = 3.72ADD118 pKa = 3.77EE119 pKa = 4.0YY120 pKa = 11.54VYY122 pKa = 11.16LLEE125 pKa = 4.98AVLSFEE131 pKa = 5.7GVDD134 pKa = 3.46GWSEE138 pKa = 3.81DD139 pKa = 3.99LAWGLTNEE147 pKa = 4.98EE148 pKa = 4.07YY149 pKa = 10.8QIACPGCEE157 pKa = 3.44VDD159 pKa = 5.75LFIALGEE166 pKa = 3.91RR167 pKa = 11.84GYY169 pKa = 11.17FSTSGDD175 pKa = 3.54YY176 pKa = 11.04ALSDD180 pKa = 3.98DD181 pKa = 4.89VPTEE185 pKa = 3.92PLRR188 pKa = 11.84PADD191 pKa = 3.78PTDD194 pKa = 3.55PAGLTGIGRR203 pKa = 11.84RR204 pKa = 11.84LHH206 pKa = 6.19DD207 pKa = 3.47TALADD212 pKa = 3.81NQQGVAKK219 pKa = 10.41ALTYY223 pKa = 10.96VFGDD227 pKa = 4.06ATCPDD232 pKa = 3.95CAEE235 pKa = 4.72DD236 pKa = 4.4FSVAAQISADD246 pKa = 3.35

Molecular weight: 26.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C2ATA5|A0A0C2ATA5_9ACTN AraC family transcriptional regulator OS=Streptomyces sp. AcH 505 OX=352211 GN=HY68_24430 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6837

71

6908

2123277

22

9675

307.4

32.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.336 ± 0.041

0.725 ± 0.009

6.138 ± 0.027

5.493 ± 0.029

2.863 ± 0.017

9.404 ± 0.029

2.229 ± 0.015

3.454 ± 0.024

2.384 ± 0.025

10.197 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.013

1.966 ± 0.017

5.814 ± 0.023

2.945 ± 0.018

7.553 ± 0.033

5.318 ± 0.025

6.258 ± 0.026

8.462 ± 0.026

1.49 ± 0.015

2.155 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski