Mosquito VEM Anellovirus SDRB A
Average proteome isoelectric point is 7.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F6KID5|F6KID5_9VIRU Capsid protein OS=Mosquito VEM Anellovirus SDRB A OX=1034792 PE=3 SV=1
MM1 pKa = 7.14 SQTTEE6 pKa = 3.48 RR7 pKa = 11.84 FLNFKK12 pKa = 10.33 KK13 pKa = 10.67 KK14 pKa = 9.59 EE15 pKa = 3.68 ALFRR19 pKa = 11.84 KK20 pKa = 6.93 TVRR23 pKa = 11.84 AAHH26 pKa = 6.28 QLFCNCEE33 pKa = 4.02 DD34 pKa = 3.96 PVQHH38 pKa = 6.58 LLGWRR43 pKa = 11.84 TTIPGEE49 pKa = 4.43 GIVGTVDD56 pKa = 3.48 TSQEE60 pKa = 3.86 HH61 pKa = 6.84 AEE63 pKa = 4.38 PGDD66 pKa = 3.84 AEE68 pKa = 4.32 LLEE71 pKa = 4.13 EE72 pKa = 5.65 LIDD75 pKa = 3.79 AGIRR79 pKa = 11.84 AGGG82 pKa = 3.23
Molecular weight: 9.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.217
IPC2_protein 5.258
IPC_protein 5.118
Toseland 5.143
ProMoST 5.283
Dawson 5.143
Bjellqvist 5.245
Wikipedia 5.003
Rodwell 5.08
Grimsley 5.092
Solomon 5.143
Lehninger 5.105
Nozaki 5.283
DTASelect 5.385
Thurlkill 5.169
EMBOSS 5.092
Sillero 5.359
Patrickios 4.329
IPC_peptide 5.156
IPC2_peptide 5.372
IPC2.peptide.svr19 5.328
Protein with the highest isoelectric point:
>tr|F6KID6|F6KID6_9VIRU Uncharacterized protein OS=Mosquito VEM Anellovirus SDRB A OX=1034792 PE=4 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 YY3 pKa = 9.65 SKK5 pKa = 10.57 QRR7 pKa = 11.84 TSTQMVSSLGVPLQEE22 pKa = 4.57 LLNLISPQKK31 pKa = 9.2 TRR33 pKa = 11.84 PGYY36 pKa = 9.39 KK37 pKa = 9.31 KK38 pKa = 9.99 RR39 pKa = 11.84 RR40 pKa = 11.84 SGAQSNIKK48 pKa = 9.77 EE49 pKa = 4.24 DD50 pKa = 3.6 QEE52 pKa = 4.37 KK53 pKa = 10.6 EE54 pKa = 3.95 NEE56 pKa = 4.46 GYY58 pKa = 10.45 YY59 pKa = 10.05 PPRR62 pKa = 11.84 AKK64 pKa = 10.28 KK65 pKa = 9.78 KK66 pKa = 9.7 RR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 TTALPRR75 pKa = 11.84 RR76 pKa = 11.84 TRR78 pKa = 11.84 KK79 pKa = 7.39 HH80 pKa = 4.9 HH81 pKa = 5.7 KK82 pKa = 9.61 RR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 SQQGDD90 pKa = 3.55 STDD93 pKa = 3.62 TEE95 pKa = 4.42 EE96 pKa = 5.89 SSEE99 pKa = 4.15 SWSSSWTEE107 pKa = 3.49
Molecular weight: 12.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.823
IPC_protein 10.613
Toseland 10.891
ProMoST 10.584
Dawson 10.965
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 11.242
Grimsley 11.008
Solomon 11.111
Lehninger 11.067
Nozaki 10.862
DTASelect 10.672
Thurlkill 10.877
EMBOSS 11.301
Sillero 10.891
Patrickios 10.965
IPC_peptide 11.111
IPC2_peptide 9.385
IPC2.peptide.svr19 8.615
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
770
82
581
256.7
30.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.065 ± 0.714
1.169 ± 0.315
4.026 ± 0.315
6.494 ± 1.427
3.506 ± 0.943
6.753 ± 0.633
2.208 ± 0.287
4.156 ± 0.627
6.494 ± 1.01
7.792 ± 0.568
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.558 ± 0.084
3.766 ± 0.525
5.844 ± 0.54
4.805 ± 0.828
10.13 ± 1.037
7.532 ± 1.671
7.143 ± 0.621
3.766 ± 0.492
3.896 ± 1.081
3.896 ± 0.914
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here